Profile of malondialdehyde (MDA) and catalase specific activity in plasma of elderly woman

Hardiany, Novi Silvia; Sucitra, Sucitra; Paramita, Reni

doi:10.22435/hsji.v12i2.2239

Cited by 15 publications

(11 citation statements)

References 13 publications

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“…One of the most damaging effects of ROS is lipid peroxidation, and the end product is Malondialdehyde (MDA) (Phaniendra et al, 2015). A high concentration of MDA proves the low antioxidant status so that it cannot prevent the reactivity of free radical compounds and enter the blood vessels (Hardiany et al, 2020). Lipid peroxide can also convert ROS into reactive compounds capable of crosslinking Deoxyribonucleic Acid (DNA) proteins and induce cell apoptosis.…”

Section: Medical Laboratory Technology Journalmentioning

confidence: 99%

Cowpea (Vigna unguiculata) Extract Reduce Malondialdehyde Levels and Prevent Aortic Endothelial Cell Decline in Ovariectomized Rats

et al. 2021

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Estrogen has anti-oxidative and anti-inflammatory properties, but its levels decrease in postmenopausal women who can trigger oxidative stress. One of the most damaging effects of ROS is lipid peroxidation, and the end product is Malondialdehyde (MDA). Similarly, aging endothelium has increased oxidative stress and endothelial cell sensitivity to apoptosis. This study aimed to determine the effect of cowpea extract on serum MDA levels, aortic endothelial cell counts, and brain MDA levels in the ovariectomy model. Cowpea extract can be used as an alternative to prevent and overcome the effects that occur during menopause, such as cardiovascular problems, decreased bone mineral density, and dementia. The study used 15-month-old female Rattus norvegicus, divided into six groups (OVX, SHAM, OVX+estradiol, OVX+Vu 1.25; 2.5; and 5 mg/kg BW/day). Serum and brain MDA levels were examined by ELISA method, while the number of aortic endothelial cells were examined on histopathological preparations with Hematoxylin & Eosin (HE) staining. The mean value of serum and brain MDA levels decreased with an increase in the dose given (p-value 0.016). The mean value of aortic endothelial cells between the dose groups did not significantly differ. However, the mean value showed an increasing trend as the dose of cowpea extract was given. The results of this study indicate that the extract of cowpea has the potential as an antioxidant to reduce serum and brain MDA levels, prevent a decrease in the number of aortic endothelial cells. As prevention, cowpea extract can be used as an antioxidant and consumed since premenopause to minimize problems that occur during postmenopause.

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Section: Medical Laboratory Technology Journalmentioning

confidence: 99%

Cowpea (Vigna unguiculata) Extract Reduce Malondialdehyde Levels and Prevent Aortic Endothelial Cell Decline in Ovariectomized Rats

et al. 2021

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“…Pemeriksaan kadar MDA melalui metode spektofotometer dengan menilai dua molekul asam tiobarbiturat (TBA) berikatan dengan satu molekul MDA menghasilkan komplek TBA-MDA-TBA berwarna merah muda yang mempunyai serapan maksimum pada panjang gelombang 530 nm. Kadar MDA plasma akan dinilai dengan satuan nmol/mL (15,16).…”

Section: Pengumpulan Dan Pengukuran Dataunclassified

Pengaruh puasa intermiten 5:2 terhadap kadar malondialdehida pada karyawan pria dewasa dengan obesitas

Yudhistina

Prafiantini

Hardiany

2021

Jurnal Gizi Klinik Indonesia

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Effect of intermittent fasting 5:2 on plasma malondialdehyde levels in adult male employees with obesityBackground: Obesity is a major risk factor for various non-communicable diseases such as type 2 diabetes mellitus and cardiovascular disease. In obesity, the free radical product will be produced, such as malondialdehyde (MDA) which plays a role in the pathogenesis of various diseases. Intermittent fasting (IF) is an alternative way to reduce free radical levels in the body therefore it can prevent complications of obesity. However, the effect of IF on MDA level in an obese adult male in Jakarta has not been reported.Objective: The objective of this study was to determine the effect of IF on malondialdehyde levels in adult male employees with obesity in Jakarta.Methods: This study used a randomized controlled clinical trial. The subjects were men aged 19-59 years with a body mass index (BMI) ≥ 25 kg/m2, divided into fasting and control groups, each group consists of 25 subjects. Intermittent fasting 5:2 is calorie restriction up to 20-25% was done every Monday and Thursday, and not allowed to eat and drink during 14 hours of fasting. Before the intervention, both groups were given education about balanced nutrition. Association of intermittent fasting with MDA and catalase was analyzed by means of difference formula with a significance limit of 5%.Results: MDA levels post-intervention significantly decreased compared to the pre-intervention both in the fasting group [1,3(0,9-2) nmol/mL to 0,4(0,3-0,6) nmol/mL] and in the control groups [1,4(0,9-1,9) nmol/mL to 0,5(0,3-0,7) nmol/mL]. MDA level post-intervention in the fasting group was significantly lower (p<0,05) compared to the control group.Conclusions: Intermittent fasting 5:2 during 8 weeks can decrease MDA levels in adult males with obesity in Jakarta.

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“…As research by Clifford et al found that betalain compounds in red beetroot extract can inhibit linoleic damage by cytochrome C oxidase and the oxidation of lipids membrane by metmyoglobin that activated H2O2 and Fe. Furthermore, the study found that betalain in red beetroot extract could inhibit lipid peroxidation due to ROS very effectively (Clifford et al, 2015;Hardiany et al, 2020) The betalain compounds in beetroot extract also function as potent anti-inflammatory agents by an interfering mechanism with pro-inflammatory signaling cascades (Clifford et al, 2015;Martinez et al, 2015;Moreno-Ley et al, 2021). Another important mechanism of the anti-inflammatory effect of red beetroot extract is to weaken the activity of the Nuclear Factor-Kappa B (NF-B) cascade, which is the agent capable of activating and transcribing some of the target genes that regulate and amplify the inflammatory response, such as cytokines, chemokines, cell apoptosis and phagocytic which can cause chronic tissue damage (El Gamal et al, 2014) The purpose of this study was to determine the effect of red beetroot extract in preventing the TNFinflammatory factor and the increasing sunburn of epidermal cells in rats exposed to UVB.…”

Section: Introductionmentioning

confidence: 99%

The extract of Red Beetroot (Beta vulgaris L.) Prevent Tumor Necrosis Factor-Alpha (TNF-?) Levels and Prevent Increased Sunburn in Epidermal Cell in Rats Exposed to UVB

Tanjung¹,

Ruma²,

Dewi³

2022

IJRP

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Backgrounds: UVB radiation can also cause acute changes, such as sunburn and pigmentation, and chronic changes, such as photocarcinogenesis and immunosuppression. The betalain compound in beetroot extract also functions as a potent anti-inflammatory agent. Method: This study employed a true experimental study with a randomized post-test only control group design using 30 rats divided into three groups for treat 28 days. The negative control used placebo and without exposure to UVB (K1). The positive control group exposed to UVB and get placebo (K2). The treatment group get exposed to UVB light and treated red beetroot extract at a dose of 500 mg/kg BW via oral (K3). TNF-levels were measured using ELISA. Sunburn cells were measured by observing the epidermal cells under a microscope. Statistical using one-way ANOVA with significantly p<0.05. Results: TNF-levels in K1 was 33.464±1.533 ng/L, K2 was 47.546±4.194 ng/L, and K3 was 32.625±0.839 ng/L. The number of sunburn cells in K1 was 0.280±0.611 cells/visual field K2 was 0.980±0.121 cells/HPF, and K3 was 0.300±0.054 cells/HPF. Based on the results of the Post-hoc (LSD) analysis, TNF-levels in K3 decreased significantly compared to K2 (p=0.000), while TNF-levels in K3 were not significantly different from K1 (p=0.824). Similarly, the number of sunburn cells in K3 decreased significantly compared to K2 (p=0.000), while the number of sunburn cells in K3 did not differ significantly from K1 (p=0.665). Conclusion: red beetroot extract can prevent inflammatory factor TNF-and increased sunburn of epidermal cells in rats exposed to UVB.

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Profile of malondialdehyde (MDA) and catalase specific activity in plasma of elderly woman

Cited by 15 publications

References 13 publications

Cowpea (Vigna unguiculata) Extract Reduce Malondialdehyde Levels and Prevent Aortic Endothelial Cell Decline in Ovariectomized Rats

Cowpea (Vigna unguiculata) Extract Reduce Malondialdehyde Levels and Prevent Aortic Endothelial Cell Decline in Ovariectomized Rats

Pengaruh puasa intermiten 5:2 terhadap kadar malondialdehida pada karyawan pria dewasa dengan obesitas

The extract of Red Beetroot (Beta vulgaris L.) Prevent Tumor Necrosis Factor-Alpha (TNF-?) Levels and Prevent Increased Sunburn in Epidermal Cell in Rats Exposed to UVB

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