Profile of fatty acids, vitamins, phytosterols and phenolic acids in Trachystemon orientalis L. and evaluation of their antioxidant activity

Abstract

The present study focuses on the phytochemical content and antiradical properties of Trachystemon orientalis L., which grown spontaneously and consumed as food in rural areas of Düzce (from Turkey) province. DPPH, ABTS and OH radical scavenging tests were carried for identifying the antiradical properties of the extracts of this plant. In addition, the metal chelating potential of this plant was also evaluated. The antioxidant activity was considered and interpreted based on the level of the % inhibition value. The average ABTS radical cleaning activities of T. orientalis extracts of methanol, ethanol and pure water were found to be 93.35%, 91.32%, 94.70%, respectively. The average DPPH radical cleaning activites of this plant extracts of methanol, ethanol and pure water were determined as 37.26%, 7.95%, 52.26%, respectively. T. orientalis the OH radical cleaning test results of the extracts of plant prepared from methanol, ethanol and pure water were determined as 60.85%, 61.33%, 24.12%, respectively. In addition, the metal chelation test results of methanol, ethanol and pure water extracts of this plant were found to be 63.57%, 42.04%, 82.64%. It was determined that the highest content of protein (20.93 mg BSA/g), proanthocyanidin (13.76 mg CE/g) and phenolic (3621.03 µg GAE/g) was found in pure water extract of T. orientalis. It has been determined that gallic acid (187.67 μg/g), vanillic acid (3.78 μg/g), rosmarinic acid (10.55 μg/g) and hydrocynamic acid (0.78 μg/g) are found in different proportions in T. orientalis plant. It has been observed that T. orientalis is an important source of palmitic acid, stearic acid, oleic acid, linoleic acid, γ-linolenic acid, alphalinolenic acid, gadoleic acid and docosahexaenoic acid. It has been established that this plant has a low content of fat-soluble vitamins and phytosterols.

Effect of heating processes on bioactive properties, phenolic components and mineral amounts of rocket (Eruca sativa Mill.) leaves

“…Heimler et al (2007) reported that the average scavenging activity in both fresh wild rocket leaves was found between approximately 20% to 35% at ~7 mg mL À1 . In another previous study, the antioxidant activity results of watercress and mizuna leaves using DPPH were higher than those of rocket leaves (120 mg/100 g FW) (Demir, 2022). In another study, Italian and Bulgarian E. sativa contained 3.62 and 4.45 mgGAE/100 g of total phenols (Matev et al, 2018).…”

“…It has been reported that there are losses in antioxidant activity values in plants as a result of antioxidant enzyme activities that lead to loss of enzymes that degrade phenolic compounds (Chan et al ., 2009). The antioxidant activity results of methanol, ethanol and pure water extracts of Trachystemon orientalis plant were monitored as 37.26%, 7.95% and 52.26%, respectively (Demir, 2022). In another study, Italian and Bulgarian E. sativa contained 3.62 and 4.45 mgGAE/100 g of total phenols (Matev et al ., 2018).…”

“…The decrease in the amount of phenolic components in rocket with drying is probably due to the deterioration of the structure of the components as a result of the applied heat treatment, some biochemical reactions and enzyme activities. The T. orientalis plant contains 187.67 gallic acid, 3.78 vanillic acid, 10.55 rosmarinic acid and 0.78 μg g −1 hydrocinnamic acid (Demir, 2022). The amounts of phytochemical and volatile organic compounds in plants can be affected by changes in environmental factors such as temperature and humidity (Bell et al ., 2016).…”

Total Antioxidant Potential, Total Phenolic Profile and Cytotoxic Activity Against Brain Cancer: Melocan and Galdirik

“…This could be related to the properties of the extract or the HPLC measurement conditions. Only rutin and myricetin were detected in abundance in infusions of galdirik leaves and stems ( 35 ) and in another paper gallic, vanillic and rosmarinic acids were reported in galdirik ( 36 ).…”

Modern and traditional cooking methods affect the antioxidant activity and phenolic compounds content of Trachystemon Orientalis (L.) G. Don

“…The mean total phenolic content of raw Trachystemon orientalis (L.) G. Don was 4.83±0.32 mg GAE/g DW. Total phenolic contents of Trachystemon orientalis (L.) G. Don samples collected from Du ¨zce and Sinop at any time of spring were reported to be in the range of 0.75±0.32-472.22±5.50 mg GAE/g DW [4,5,30]. This difference in total phenolic content may have been due to conditions such as soil structure, sun intake, climate characteristics, and harvest time of the plant [31].…”