Profile of 25-Hydroxyvitamin D in Individuals Attending Armed Forces Institute of Pathology (AFIP), Dhaka

Abstract

Background: In the last two decades, there has been increasing interest in the biology of vitamin D and a growing recognition is that vitamin D deficiency is common in tropical countries 25-OH vitamin D level in serum was estimated by electro-chemiluminescence on Cobas Elecsys e411 fully automated system on the day of collection. This method has been standardized against LC-MS/MS which in turn has been standardized to the NIST (National Institute of Standards and Technology, USA). The functional sensitivity was determined to be 4.01 ng/mL (CV 18.5%). Vitamin D deficiency is defined as 25(OH) D <50 nmol/L, insufficiency as 50-75 nmol/L and sufficiency as ≥75 nmol/L and toxicity >375 nmol/L. The definition of vitamin D status was based on the recommendation of the US Institute of Medicine (IOM) for vitamin D.