Profile and protective role of 61kDa and 47kDa antigens of Edwardsiella tarda in rohu (Labeo rohita)

Abstract

Edwardsiella tarda is a versatile pathogen that could survive in different environmental conditions and infect economically important fish species. The whole cell antigenic proteins were extracted from E. tarda and assessed via Western blot using anti E. trada rohu and anti E. trada rabbit serum. Two strong reacted proteins with antibodies were recovered viz 61 kDa and 47 kDa from SDS PAGE gels to evaluate their vaccine potential in rohu fish. Fish were vaccinated 10 µg of antigenic proteins intraperitoneally as immunogens with Freund's incomplete adjuvant (FIA). The boosters were given on the 10 th day of immunization with PBS. The blood was drawn from vena caudalis at every 7 th day for hematological and immunological studies. Fish were challenged on 35 th day with 1× 10 6 cfu E. tarda field isolate ET-1.

Assessment of immune responses in Indian major carp, Labeo rohita against multiple antigenic components of three bacterial fish pathogens; Aeromonas hydrophila , Pseudomonas aeruginosa and Staphylococcus aureus

“…However, bacterial diseases due to Aeromonas , Edwardsiella , Pseudomonas , S taphylococcus , Flavobacterium , etc ., are mainly responsible for severe mortality and morbidity in cultured fish species (Mishra, Das, Choudhary, et al, 2017; Mishra, Das, Dhiman, et al, 2017; Nayak, 2020). Most of the earlier studies focused to develop monovalent‐based vaccines against these pathogens (Bootsma et al, 1977; Kasagala et al, 2016; Kumar et al, 1986; Mumtaj and Brindhdevi 2016; Rajalakshmi and Brindha Devi 2016a,b; Shome & Shome, 1999). While protecting the cultured fish simultaneously against multiple pathogens remain the challenges in aquaculture, very limited attempt has been made to develop polyvalent vaccine.…”