Profile analysis of circRNAs in human THP-1 derived macrophages infected with intracellular Staphylococcus aureus

Xie, Xiaolu; Chen, Zhihao; Han, Mingxiao; Wang, Xi; Wang, Min; Lv, Jingnan; Xie, Xing; Zhai, Yaxuan; Li, Liubing; Du, Hong; Xie, Zonggang; Zhang, Hai-Fang

doi:10.1016/j.micpath.2022.105466

Cited by 2 publications

(2 citation statements)

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“…The pathological inflammation of eukaryotic cells caused by S. aureus infection, especially in macrophages, is extremely poor in terms of antibiotic availability. 28 By comparing the ability of penicillin and composites to kill S. aureus infected with RAW264.7, it was found that after 2 and 4 h of incubation with PW-2 and PW-2@HA, the survival rate of bacteria decreased to 53.16%, 51.08%, 13.15%, and 17.62%, respectively, which indicated that the bacterial survival rate was close to 100% after penicillin treatment (Figure 4L,M). Furthermore, we explored the pathway by which the PTDs are transported into RAW264.7 cells by treating them with chlorpromazine (an inhibitor of clathrin-mediated endocytosis), amiloride (an inhibitor of endocytosis), MβCD (a disruptor of lipid rafts), and nocodazole (an inhibitor of tubulin synthesis of microtubules) to inhibit cellular transport units.…”

Section: Resultsmentioning

confidence: 90%

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Protein Transduction System Based on Tryptophan-zipper against Intracellular Infections via Inhibiting Ferroptosis of Macrophages

Fang

Sui

et al. 2023

ACS Nano

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Cells penetrating molecules in living systems hold promise of capturing and eliminating threats and damage that can plan intracellular fate promptly. However, it remains challenging to construct cell penetration systems that are physiologically stable with predictable self-assembly behavior and well-defined mechanisms. In this study, we develop a core–shell nanoparticle using a hyaluronic acid (HA)-coated protein transduction domain (PTD) derived from the human immunodeficiency virus (HIV). This nanoparticle can encapsulate pathogens, transporting the PTD into macrophages via lipid rafts. PTD forms hydrogen bonds with the components of the membrane through TAT, which has a high density of positive charges and reduces the degree of membrane order through Tryptophan (Trp)-zipper binding to the acyl tails of phospholipid molecules. HA-encapsulated PTD increases the resistance to trypsin and proteinase K, thereby penetrating macrophages and eliminating intracellular infections. Interestingly, the nonagglutination mechanism of PTD against pathogens ensures the safe operation of the cellular system. Importantly, PTD can activate the critical pathway of antiferroptosis in macrophages against pathogen infection. The nanoparticles developed in this study demonstrate safety and efficacy against Gram-negative and Gram-positive pathogens in three animal models. Overall, this work highlights the effectiveness of the PTD nanoparticle in encapsulating pathogens and provides a paradigm for transduction systems-anti-intracellular infection therapy.

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Section: Resultsmentioning

confidence: 90%

“…The pathological inflammation of eukaryotic cells caused by S. aureus infection, especially in macrophages, is extremely poor in terms of antibiotic availability . By comparing the ability of penicillin and composites to kill S.…”

Section: Resultsmentioning

confidence: 99%