Proenergetic effects of resveratrol in the murine neuronal cell line Neuro2a

Nguyen, Nhu T. Q.; Ooi, Lezanne; Piller, Sabine C.; Münch, Gerald

doi:10.1002/mnfr.201300145

Cited by 9 publications

(4 citation statements)

References 39 publications

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“…In the primary cultured taste buds cells, preliminary analysis with 3 μM RSVA314 resulted in an inconsistent T1r2 expression between each experiment (data not shown). In the murine Neuro 2a cell line, resveratrol was reported to increase adenine nucleotide pools [12]. Next, we analyzed the effect of adenine-related compounds on T1r2 transcript levels of MIN 6 cells and the primary cultured taste buds cells.…”

Section: Resveratrol and Rsva314 Effects On T1r's Expressionmentioning

confidence: 99%

“…In INS-1-1E β-cells-another insulin secreting cell lines upon glucose stimulation-resveratrol was reported to potentiate insulin-secretion in the medium with the 15 mM, 20 mM and 30 mM glucose concentrations through the adenine mononucleotide-activated protein kinase (AMPK) pathway [6]. Resveratrol has also been shown to prevent type 2 diabetes by increasing insulin sensitivity [7] [8] and/or by decreasing IGF-1 [9] [10], and to protect neurodegeneration [11] probably through the AMPK [9] and other pathways [12] [13].…”

Section: Introductionmentioning

confidence: 99%

“…Adenine is known to be rich in soybean [14]. Adenine and adenine-related compounds are reported to modulate AMPK activity [15] [16], and resveratrol was reported to increase the amounts of adenine-nucleotidetriphosphate (ATP) and those of guanine-nucleotide-triphosphate (GTP) in Neuro 2A cell lines [12]. The effects of resveratrol and adenine-related compounds on the expression of sweet receptors remained unknown.…”

Section: Introductionmentioning

confidence: 99%

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Nutrients Modulate T1r2 Transcript Levels in MIN 6 and Primary Cultured Taste Buds Cells under High Glucose Condition

Nakmura¹,

Ookura²

2016

FNS

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The sweet taste receptors comprised of T1r2 and T1r3, sense glucose concentrations in the gastrointestine. While hyperglycemia was reported to decrease the T1R2 and T1R3 tanscript levels in healthy subjects, no change was observed in type 2 diabetes patients. We investigated which glucose level and nutrients affect those transcript levels in MIN 6 and primary cultured taste buds cells using quantitative Reverse Trancription Polymerase Chain Reaction. High glucose diminished T1r2 transcript levels in MIN 6 and primary cultured taste buds cells. Resveratrol and its analogue augmented transcript levels of T1r1 and T1r2 above normal levels in MIN 6 cells in the medium with 25 mM glucose. Adenine, but not guanine, augmented T1r2 transcript levels of MIN 6 cells in the medium with 25 mM glucose. These results imply that nutrients in meals could affect sweet taste sensitivity by modulating T1r2 transcript levels in response to blood glucose levels.

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Section: Resveratrol and Rsva314 Effects On T1r's Expressionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Nutrients Modulate T1r2 Transcript Levels in MIN 6 and Primary Cultured Taste Buds Cells under High Glucose Condition

Nakmura¹,

Ookura²

2016

FNS

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“…Entretanto, estudos mais recentes abordaram a regulação da transcrição do fator neurotrófico mesencefálico derivado astrócitos nas células neuro2A [29] . Outros, entretanto, avaliaram os efeitos de oxidantes na adenosina monofosfato quinase ativada (AMPK) sobre o metabolismo energético destas células [30] . Desta forma, tendo-se em vista a pouca quantidade de trabalhos utilizando-se as células neuro2a e o uso de biomateriais, o propósito deste estudo será avaliar a capacidade dos biopolímeros poli caprolactona (PCL) e poli L-ácido lático (PLLA) na adesão e proliferação das mesmas in vitro.…”

Section: Introductionunclassified

Estudo das células Neuro2A sobre os biomateriais PCL e PLLA

et al. 2014

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Resumo: Os biomateriais poli L-ácido lático (PLLA) e o poli caprolactona (PCL) são os polímeros mais estudadas na área dos materiais bioreabsorvíveis. Dentre as suas principais características que contribuem para a interação celular, temos a especificidade química da superfície, elétrica, hidrofobicidade e topografia. Ainda, observa-se o tempo de degradação, porosidade, biocompatibilidade com o tecido biológico, bem como, a confecção com as mais variadas formas e dimensões. Já a prática da cultura celular, tem como objetivo estudar a adesão, migração, diferenciação e a proliferação celular utilizando-se um determinado material ou substância. Contudo, poucos trabalhos utilizando os biomateriais ora supracitados e a aplicação em células neuro2A foram realizados. Sabe-se que este tipo celular é derivado de células embrionárias da crista neural, as quais originam em neurônios simpáticos e apresentam como característica a imortalidade, portanto, são excelentes modelos em ensaios in vitro. Nesse sentido, o presente estudo avalia a adesão e a proliferação desta linhagem celular sobre os biopolímeros poli caprolactona (PCL) e poli L-ácido lático (PLLA). Palavras-chave: Biomateriais, PLLA, PLC, neuroblastoma, cultura. Study of Neuro2a Cells on the Biomaterials Poly L-lactic Acid and Poly CaprolactoneAbstract: Biomaterials poly L-lactic acid (PLLA) and poly caprolactone (PCL) are the most studied in the area of bioresorbable materials. Among the main features that contribute to cell interaction, we have the specific surface chemistry, electrical, hydrophobicity and topography. Also, is observed the degradation time, porosity, biocompatibility with biological tissue, as well as the preparation of the most varied shapes and sizes. The practice of cell culture, aims to study the adhesion, migration, differentiation and cell proliferation using a given material or substance. However, few studies were performed using these biomaterials and the application of neuro2A cells. It is known that this cell type is derived from the embryonic neural crest cells, which originate in sympathetic neurons and have the characteristic of immortality, therefore, are excellent models in vitro assays. Accordingly, the present study evaluates the adhesion and proliferation of this cell line on the biopolymers poly caprolactone (PCL) and poly L-lactic acid (PLLA). Glória, 187, Centro, Diamantina, MG, Brasil, Keywords: Biomaterials, PLLA, PLC, neuroblastoma, culture. Autor para correspondência: Amauri Pierucci, Departamento de Ciências Básicas, Universidade Federal dos Vales do Jequitinhonha e Mucuri -UFVJM, Rua da IntroduçãoCom o advento da bioengenharia intensificaram os estudos sobre a aplicação e ação dos biomateriais em diferentes tipos celulares. Sabe-se que a adesão, migração, diferenciação e a proliferação celular sobre determinado substrato depende em parte das propriedades intrínsecas de cada tipo celular, bem como, o tipo de material utilizado [1][2][3][4] . Dentre os produtos celulares, pode-se ressaltar a síntese de moléculas de adesã...

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Resveratrol and Alzheimer’s Disease: Mechanistic Insights

et al. 2016

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Alzheimer's disease (AD) is the leading cause of dementia in the elderly and is characterized by progressive cognitive and memory deficits. The pathological hallmarks of AD include extracellular senile plaques and intracellular neurofibrillary tangles. Although several mechanisms have been used to explain the underlying pathogenesis of AD, current treatment regimens remain inadequate. The neuroprotective effects of the polyphenolic stilbene resveratrol (3,5,4'-trihydroxy-trans-stilbene) have been investigated in several in vitro and in vivo models of AD. The current review discusses the multiple potential mechanisms of action of resveratrol on the pathobiology of AD. Moreover, due to the limited pharmacokinetic parameters of resveratrol, multiple strategies aimed at increasing the bioavailability of resveratrol have also been addressed.

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Proenergetic effects of resveratrol in the murine neuronal cell line Neuro2a

Cited by 9 publications

References 39 publications

Nutrients Modulate T1r2 Transcript Levels in MIN 6 and Primary Cultured Taste Buds Cells under High Glucose Condition

Nutrients Modulate T1r2 Transcript Levels in MIN 6 and Primary Cultured Taste Buds Cells under High Glucose Condition

Estudo das células Neuro2A sobre os biomateriais PCL e PLLA

Resveratrol and Alzheimer’s Disease: Mechanistic Insights

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