Productive Infection of Bacillus Subtilis 168, With Bacteriophage Sp-10, Dependent Upon Inducing Treatments

Goldberg, Ivan D.; Bryan, Theodore

doi:10.21236/ad0686354

Cited by 11 publications

(2 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…SP-15 lysates prepared in this medium retained good transducing ability for several months when stored without additions at 4 C. The high-titer SP-10 used for antiserum production was propagated in TY broth (18) by the method of Goldberg and Bryan VOL. 100, 1969 COTRANSDUCTION AND COTRANSFORMATION IN BACILLUS (11). Phage PBS-1 was provided by J. Spizizen and W. R. Romig.…”

Section: Methodsmentioning

confidence: 99%

Cotransduction and Cotransformation of Genetic Markers in Bacillus subtilis and Bacillus licheniformis

Tyeryar¹,

Taylor²,

Lawton³

et al. 1969

J Bacteriol

Self Cite

View full text Add to dashboard Cite

Bacteriophage SP-15, a large generalized transducing phage of Bacillus, was compared with phages PBS-1 and SP-10 for the ability to cotransduce pairs of genetic markers exhibiting different degrees of linkage. When auxotrophs of B. subtilis W-23 were used as recipients, SP-15 and PBS-1 effected a much higher frequency of cotransduction than did SP-10 with markers that were not closely linked. With more closely linked loci, the differences were not as great. SP-15 cotransduced linked markers at a higher mean frequency than PBS-1, suggesting that SP-15 is able to transfer a larger fragment of the Bacillus genome than any phage heretofore described. The frequency of the joint transfer of genetic markers in B. licheniformis was lower via transforming deoxyribonucleic acid than by transduction with phage SP-10. The availability of three procedures for genetic exchange-transduction by SP-15 and SP-10 as well as transformation-each of which reveals a different degree of linkage, makes B. licheniformis 9945A especially amenable to genetic analysis. Recently, Tyeryar et al. (28) demonstrated in Bacillus licheniformis that for a given pair of genetic markers the frequency of cotransduction by phage SP-15 was higher than the frequency (23,24). Peptone diluent contained 10 g of Difco 1027on July 4, 2020 by guest

show abstract

Section: Methodsmentioning

confidence: 99%

Cotransduction and Cotransformation of Genetic Markers in Bacillus subtilis and Bacillus licheniformis

Tyeryar¹,

Taylor²,

Lawton³

et al. 1969

J Bacteriol

Self Cite

View full text Add to dashboard Cite

show abstract

“…Cells of Bacillus subtilis Marburg strain can adsorb phage SP1O but are nonpermissive to the replication of this phage and thus the Marburg strains can not serve as gene donors in generalized transduction by phage SP1O (1). GWINN and LAWTON (2) and GoLDBERG and BRYAN (3) reported that the cells of Marburg strain were rendered permissive to infection with phage SP1O by heating or mitomycin C treatment, and suggested that repressors of endogenous defective prophage PBSX may play a role in the nonpermissiveness of the B. subtilis Marburg strain.…”

mentioning

confidence: 99%

Mechanisms of nonpermissiveness in abortive infection of bacteriophage .PHI.NR2 in Bacillus subtilis Marburg strain.

Yuki

Saito

Ikeda

1980

J. Gen. Appl. Microbiol.

View full text Add to dashboard Cite

A mutant strain PS9, permissive to infection of phages SP1O and ¢NR2, was derived from a nonpermissive Marburg strain of Bacillus subtilis. When treated with mitomycin C or ultraviolet irradiation, PS9 cells lysed to produce defective phage PBSX as did the nonpermissive cells. Thus it was suggested that the nonpermissiveness might be independent of the repression system of the defective phage PBSX. As another approach to elucidate the mechanism of nonpermissiveness, DNA and RNA syntheses were analyzed in both permissive and nonpermissive cells infected with phage c N R2. By molecular hybridization with phage DNA, it was found that, in the nonpermissive cells, cNR2 DNA did not replicate but phage-directed RNA was partially synthesized.The RNA synthesized in the nonpermissive B. subtilis Marburg appeared to lack late messenger RNA compared to RNA synthesized in the permissive cells.Cells of Bacillus subtilis Marburg strain can adsorb phage SP1O but are nonpermissive to the replication of this phage and thus the Marburg strains can not serve as gene donors in generalized transduction by phage SP1O (1).GWINN and LAWTON (2) and GoLDBERG and BRYAN (3) reported that the cells of Marburg strain were rendered permissive to infection with phage SP1O by heating or mitomycin C treatment, and suggested that repressors of endogenous defective prophage PBSX may play a role in the nonpermissiveness of the B. subtilis Marburg strain.We have isolated a mutant of B, subtilis Marburg strain which is permissive to phage SP1O as well as a newly isolated phage NR2 (4) and attempted to study mechanisms of the nonpermissiveness in two respects. The first relates to the induction of PBSX in the permissive mutant, and the second is concerned with phage-directed DNA or RNA synthesis in nonpermissive and permissive hosts. In these studies we have used the ONR2 which is similar to phage SP1O with respect to the host specificity concerning the nonpermissiveness (5), since SP1O 291

show abstract

Isolation and characterization of bacteriophage ΦBP fromPaenibacillus polymyxaCCM 7400

Halgasova

Ugorcakova

Gerova

et al. 2010

FEMS Microbiology Letters

View full text Add to dashboard Cite

A bacteriophage PhiBP infecting Paenibacillus polymyxa CCM 7400 was isolated from culture lysate. Electron microscopy of lysate samples revealed the presence of bacteriophage particles with polyhedral heads 56 nm in diameter and flexible noncontractile tails 144 nm in length. The profile of PhiBP structural proteins resembles that of other bacteriophages. The PhiBP genome consists of double-stranded DNA of 43-kbp size. Homology search of sequenced DNA fragments from EcoRI digest revealed regions with significant similarity to other known bacteriophage genes. Regions similar to phage terminase genes were identified within the 1.2-kbp fragment. Three lytic genes, two holin genes and one endolysin gene were identified within the 2.5-kbp fragment. We tested the isolates of P. polymyxa CCM 7400 for the presence of phage DNA on bacterial chromosome using PCR amplification with primers derived from proposed terminase and holin gene sequences. We confirmed the presence of PhiBP DNA on P. polymyxa chromosome by Southern hybridization. The bacteriophage PhiBP was capable of causing lysis of a P. polymyxaPhiBP lysogen despite the presence of the phage DNA on bacterial chromosome. Therefore, we concluded that PhiBP was a virulent mutant phage.

show abstract

Productive Infection of Bacillus Subtilis 168, With Bacteriophage Sp-10, Dependent Upon Inducing Treatments

Cited by 11 publications

References 29 publications

Cotransduction and Cotransformation of Genetic Markers in Bacillus subtilis and Bacillus licheniformis

Cotransduction and Cotransformation of Genetic Markers in Bacillus subtilis and Bacillus licheniformis

Mechanisms of nonpermissiveness in abortive infection of bacteriophage .PHI.NR2 in Bacillus subtilis Marburg strain.

Isolation and characterization of bacteriophage ΦBP fromPaenibacillus polymyxaCCM 7400

Contact Info

Product

Resources

About