Cotransduction and Cotransformation of Genetic Markers in
            <i>Bacillus subtilis</i>
            and
            <i>Bacillus licheniformis</i>

Tyeryar, Franklin J.; Taylor, Martha J.; Lawton, William D.; Goldberg, Ivan D.

doi:10.1128/jb.100.2.1027-1036.1969

Cited by 36 publications

(21 citation statements)

References 27 publications

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“…Electrophoresis of digests was in 0.7% agarose as described in the text. or Fe2 , and several media reported previously, such as Minl, Min3, Min3C, Min7, and MinlO (23,24,25,26) containing various amino acids, casein, or broth supplements. No large differences were observed among several supplemented minimal media tested except that some supplements allowed too much growth of the recipient leucine auxotroph.…”

Section: Methodsmentioning

confidence: 80%

MP13, a generalized transducing bacteriophage for Bacillus megaterium.

Vary¹,

Garbe²,

Franzen³

et al. 1982

Journal of Bacteriology

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The first generalized transducing bacteriophage reported for Bac illhs inegaterium has been characterized. Optimum conditions for lysate production and transduction procedures were established so that transducing frequencies of 8 x 10-6 and higher are now possible. The phage, MP13, has a head diameter of 97 nm and a contractile tail (202 by 17 nm) and adsorbs to the periphery of the cell. MPI3 was inactivated rapidly at 60°C, but not at 55°C, and was sensitive to toluene, ether, and chloroform. When centrifuged in a neutral CsCl gradient, two bands were observed, a major band of 1.490 g cm<3 and a minor band of 1.482 g cm 3 buoyant density. The major band contained only infective particles, whereas the minor band contained both infective and transducing particles. Phage DNA was resistant to several restriction endonucleases, but yielded 9 fragments with MboI, more than 34 with HindIII, and 7 with BstEII. The molecular weights for the fragments from MboI-BstEII double digests total 97 x 109. Until the recent isolation of phage MP13 (31), no generalized transducing phage for Bacillus megaterilum was available for genetic analysis of mutants in this bacterium. We reported previ

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Section: Methodsmentioning

confidence: 80%

MP13, a generalized transducing bacteriophage for Bacillus megaterium.

Vary¹,

Garbe²,

Franzen³

et al. 1982

Journal of Bacteriology

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“…PBS1 and SP10 in heterologous exchanges give recombination values of the order of those of transformation, indicating extensive fragmentation of their DNA (respectively, of 1.9 x 108 and 5.9 x 107 molecular weight [11]) upon entering on strain 168 background. The finding of Tyeryar et al (22) in Bacillus licheniformis, that the linkage of markers was greater with SP10 transduction than in transformation, supports this hypothesis. Alternatively, it is possible that double-stranded DNA integration is involved in homologous transduction (in contrast with the single-stranded integration), clearly demonstrated in transformation, and conceivable in heterologous transduction.…”

Section: Effect Of Hpura On Pbs1 Transductionmentioning

confidence: 65%

Effect of 6-(p-hydroxyphenylazo)-uracil on the homologous and heterologous transduction processes in Bacillus subtilis

Canosi¹,

Ferrari²,

Falaschi³

et al. 1979

J Bacteriol

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We have studied the effect of 6-(p-hydroxyphenylazo)-uracil on the recombination processes that operate in the homologous and heterologous transduction mediated by PBS1 and SP10 phages of Bacillus subtilis. The results obtained demonstrate that the process of heterologous genetic exchange is sensitive to this compound, whereas the homologous process is not. The present data, along with those of our previous work (U. Canosi, A. G. Siccardi, A. Falaschi, and G. Mazza, J. Bacteriol. 126:108-121, 1976), suggest that the DNA polymerase III is involved in the recombination process that operates in transformation and heterologous transduction, whereas homologous transduction follows a partially independent pathway not involving this protein.

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“…The melting temperature of TP-13 DNA, 61°C, was extremely low for the observed density, indicating that TP-13 probably has an unusual base. These properties of TP-13 DNA are analogous to those of SP-15 DNA (61°C and 1.762 g/cm3 [25]), which is glucosylated and contains an unusual pyrimidine, 5-(4',5'-dihydroxypentyl)uracil (4,14). TP-13 and SP-15 are related serologically.…”

Section: Figmentioning

confidence: 73%

“…Observations of purified TP-13 bv electron microscopy indicated a large bacteriophage with a head diameter of approximately 120 nm and a tail length of 260 nm (Fig. 3), resembling SP-15 (25) and PBS-1 (7) in morphology and size. When phage was stained with uranyl acetate, the fine structure was not clearly defined, but _ -; > S +~~~_.…”

Section: Isolation Of Tp-13 and Conversion Of Anmentioning

confidence: 95%

Converting bacteriophage for sporulation and crystal formation in Bacillus thuringiensis

Perlak¹,

Mendelsohn²,

Thorne³

1979

J Bacteriol

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Bacteriophage TP-13, a converting phage for sporulation and crystal formation in Bacillus thuringiensis, was isolated from soil. The phage converted an oligosporogenic (sporulation frequency, 10-'), acrystalliferous mutant to spore positive, crystal positive at a high frequency. Each plaque formed by TP-13 in a lawn of sensitive cells contained spores and crystals. These spores were heat stable, and each one was capable of producing a plaque from which TP-13 could be reisolated. Conversion of cells to sporulation and crystal formation was independent of the host used for TP-13 propagation. When converted cells were cured of TP-13, they lost the ability to produce spores and crystals. Incubation of TP-13 with antiserum prepared against purified phage particles prevented conversion. TP-13 has some characteristics similar to those of SP-15 and PBS-1, including large size, morphology, and adsorption specificity of motile cells. TP-13 mediated generalized transduction in several strains of B. thuringiensis at frequencies of 10-6 to 10-5. Comparison of cotransduction values indicated that TP-13 transduced considerably larger segments of deoxyribonucleic acid than CP-51 or TP-10, two other transducing phages for B. thuringiensis.

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Cotransduction and Cotransformation of Genetic Markers in Bacillus subtilis and Bacillus licheniformis

Cited by 36 publications

References 27 publications

MP13, a generalized transducing bacteriophage for Bacillus megaterium.

MP13, a generalized transducing bacteriophage for Bacillus megaterium.

Effect of 6-(p-hydroxyphenylazo)-uracil on the homologous and heterologous transduction processes in Bacillus subtilis

Converting bacteriophage for sporulation and crystal formation in Bacillus thuringiensis

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