Production, purification and properties of microbial phytases

Pandey, Ashok; Szakács, George; Soccol, Carlos Ricardo; Rodríguez-León, José A.; Soccol, Vanete Thomaz

doi:10.1016/s0960-8524(00)00139-5

Cited by 281 publications

(179 citation statements)

References 84 publications

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“…The potential demand for phytase enzyme in swine and poultry feed is around 8,000 tonnes/annum, but the present production level is 250 tonnes/annum (Wodzinski and Ullah 1996). Phytase supplementation can reduce the amount of phosphorus in manure up to approximately 30 % (Pandey et al 2001). Hence, with increasing poultry sectors worldwide and market demand for phytase enzyme, there is need for more production of phytases with high specific activity and broad substrate specificity and their use in food and feed formulations.…”

Section: Introductionmentioning

confidence: 99%

Extracellular phytase from Aspergillus niger CFR 335: purification and characterization

Gunashree

Venkateswaran

2014

J Food Sci Technol

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Phytase, that is extensively used as a feed additive is capable of hydrolyzing phytic acid, an antinutrient found in about 60-80 % of all the plant commodities. This enzyme improves the bioavailability of essential minerals such as Ca 2+ , Mg 2+ , P, Zn 2+ , Fe 3+ , that are bound to phytic acid. An extracellular phytase from a local fungal isolate, Aspergillus niger CFR 335 was purified to homogeneity through a threestep column chromatography using DEAE-Sephadex anion exchanger. An active fraction of the enzyme was obtained with NaCl gradient of 2.5 M in DEAE Sephadex column. The enzyme was purified up to 16 fold with a yield of 28.5 %. Substrate specificity studies revealed a highest specific activity of 32.6±3.1 U/mg for sodium phytate with the K m value of 0.08±0.1 mM. The molecular weight of the enzyme was 66 kDa with an optimum temperature of 30°C and pH 4.5. Up to 80 % of the activity was retained even after storing the enzyme for 6 months at 4°C.

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Section: Introductionmentioning

confidence: 99%

Extracellular phytase from Aspergillus niger CFR 335: purification and characterization

Gunashree

Venkateswaran

2014

J Food Sci Technol

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“…Alkaline phosphatases are used in enzymelinked immunoabsorbent assays (ELISA), nonisotopic probing, blotting and sequencing systems (9). Industrial application of acid phosphatase is limited, but phytase, a type of acid phosphatase (EC 3.1.3.8) is used in animal feed (10). These enzymes catalyze the release of phosphate from phytic acid, the major phosphorus storage form in cereal grains, legumes and oilseeds.…”

Section: Introductionmentioning

confidence: 99%

Screening of filamentous fungi for production of enzymes of biotechnological interest

Guimarães¹,

Peixoto-Nogueira²,

Michelin³

et al. 2006

Braz. J. Microbiol.

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Many enzymes produced by fungi have relevant biotechnological applications in several industrial areas. The purpose of this study was to collect and isolate filamentous fungi from soil and humus, plants and sugar cane bagasse of different regions of the São Paulo state. Forty isolates were examined for their ability to produce xylanase, glucose-oxidase, alkaline phosphatase, acid phosphatase, phytase, pectinase and amylase. Among these, twenty three isolates exhibited enzymatic potential. The xylanases produced by two of these isolates (Aspergillus caespitosus and A. phoenicis) showed good potential for pulp bleaching. Among seventeen isolates, at least three produced high levels of glucose-oxidase, being Rhizopus stolonifer and A. versicolor the best producer strains. A. caespitosus, Mucor rouxii, and nine others still not identified were the best producers of phosphatases in submerged fermentation. Pectinase was best produced by IF II and C-8 belong R. stolonifer. Significant levels of amylase were produced by Paecilomyces variotii and A. phoenicis. A remarkable enzyme producer was Rhizopus microsporus var. rhizopodiformis that produced high levels of amylase, alkaline and acid phosphatases, and pectinase. Some morphological structures of this fungus were illustrated using light microscopy (LM) and scanning electron microscopy (SEM). This study contributes to catalogue soil fungi isolated in the state of São Paulo, and provides additional information to support future research about the industrial potential of these microorganisms that may produce enzymes and, eventually, also secondary metabolites with anti-microbial or anti-parasitic activities.

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“…It improves nutritional value of plant based foods by enhancing protein digestibility and mineral availability through phytate hydrolysis during food processing as well as during digestion and absorption in the intestinal region [9]. Research has proved that microbial phytases are most promising for biotechnological applications [14]. Since phytase is proven to hydrolyze phytic acids and their salts effectively, purification and characterization of novel phytase can be of significance in food and feed processing industries.…”

Section: Properties Of Phytase and Its Applicationmentioning

confidence: 99%

Phytate Solubilizing Microorganisms and Enzyme Phytase to Combat Nutritional Problems in Cereal-Based Foods

Amina

et al. 2017

JBMOA

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Phytic acid (myo-inositol hexakisphosphate) is the main storage form of phosphorus (P) in plants and accounts for more than 80 % of the total P in cereals. It is one of the sources of energy and an important mineral chelating compound in plants. However, it is a major anti-nutritional factor for human beings especially to individuals consuming large quantities of cereals like rice, finger millet and wheat. The anti-nutritional properties of phytates are known to be reduced through dephosphorylating process by means of enzyme, 'phytase' of microbial origin. Numerous studies have demonstrated the effectiveness of microbial phytase in improving the utilization and absorption of nutrients from phytate. Reduced level of phytate in foods is known to enhance the bioavailability of minerals and other associated nutrients needed for human health. Phytase is getting prime importance in processing and manufacturing industries that supply feeds for birds and non-ruminant animals. It can be a good substitute for inorganic phosphorus (Pi) supplements in poultry and animal feed, which otherwise can cause environmental pollution. Research has been conducted to prove antinutritional nature of phytic acid along with an effort to reduce its content in foods consumed. This review briefly narrates the study related to properties of phytate, application phytate solubilizing microorganisms and enzyme 'phytase' obtained from them.

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Production, purification and properties of microbial phytases

Cited by 281 publications

References 84 publications

Extracellular phytase from Aspergillus niger CFR 335: purification and characterization

Extracellular phytase from Aspergillus niger CFR 335: purification and characterization

Screening of filamentous fungi for production of enzymes of biotechnological interest

Phytate Solubilizing Microorganisms and Enzyme Phytase to Combat Nutritional Problems in Cereal-Based Foods

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