Screening of filamentous fungi for production of enzymes of biotechnological interest

Abstract

Many enzymes produced by fungi have relevant biotechnological applications in several industrial areas. The purpose of this study was to collect and isolate filamentous fungi from soil and humus, plants and sugar cane bagasse of different regions of the São Paulo state. Forty isolates were examined for their ability to produce xylanase, glucose-oxidase, alkaline phosphatase, acid phosphatase, phytase, pectinase and amylase. Among these, twenty three isolates exhibited enzymatic potential. The xylanases produced by two of these isolates (Aspergillus caespitosus and A. phoenicis) showed good potential for pulp bleaching. Among seventeen isolates, at least three produced high levels of glucose-oxidase, being Rhizopus stolonifer and A. versicolor the best producer strains. A. caespitosus, Mucor rouxii, and nine others still not identified were the best producers of phosphatases in submerged fermentation. Pectinase was best produced by IF II and C-8 belong R. stolonifer. Significant levels of amylase were produced by Paecilomyces variotii and A. phoenicis. A remarkable enzyme producer was Rhizopus microsporus var. rhizopodiformis that produced high levels of amylase, alkaline and acid phosphatases, and pectinase. Some morphological structures of this fungus were illustrated using light microscopy (LM) and scanning electron microscopy (SEM). This study contributes to catalogue soil fungi isolated in the state of São Paulo, and provides additional information to support future research about the industrial potential of these microorganisms that may produce enzymes and, eventually, also secondary metabolites with anti-microbial or anti-parasitic activities.

Production of thermostable invertases by Aspergillus caespitosus under submerged or solid state fermentation using agroindustrial residues as carbon source

“…caespitosus (21) was isolated from Brazilian soil (10) and identified by André Tosello Foundation (Campinas, SP, Brazil …”

Improvement of xylanase production by Cochliobolus sativus in solid state fermentation

“…Several enzymatic activities were investigated in isolates of the fungus Cochliobolus sativus, the causal agent of barley spot blotch disease, such as cellulose-hydrolysing enzymes (14), endo-1, 4-β-xylanase (18) and endopolygalacturonase (6). However, enzyme production is related to the type and concentrations of carbon and nitrogen sources (11,13). In the present study, we report the effects of different substrates on xylanase production by the newly Cochliobolus sativus strain Cs6 under solid state fermentation (SSF).…”

Biotechnological potential of alternative carbon sources for production of pectinases by Rhizopus microsporus var. rhizopodiformis

“…Ten fungi were isolated from the soil and plants waste from different areas of São Paulo and Paraná states, Brazil, as previously cited by Guimarães et al (2006) and these were cultivated on PDA medium at 40ºC. The investigated areas were located at S 21º09.190 ' -45º52.150 Schipper and Stalpers (1984).…”