Production, purification, and characterization of a novel serine‐esterase from Aspergillus westerdijkiae

Abstract

Esterases hydrolyze water soluble short chain fatty acids esters and are biotechnologically important. A strain of Aspergillus westerdijkiae isolated from cooking oil for recycling was found to secrete an esterase. The best enzyme production (19-24 U/ml of filtrate) culture conditions were stablished. The protein was purified using ammonium sulphate precipitation, dialysis, and a chromatographic step in Sephacryl S-200 HR. The 32 kDa purified protein presented an optimal temperature of 40°C, with a T of 48.95°C, and an optimal pH of 8.0. K and V were 638.11 µM for p-NPB and 5.47 µmol of released p-NP · min · µg of protein, respectively. The purified enzyme was partially active in the presence of 25% acetone. PMSF inhibited the enzyme, indicating that it is a serine hydrolase. MS enzyme peptides sequences were used to find the protein in the A. westerdijkiae sequenced genome. A structure model demonstrated that the protein is a member of the a/ß -hydrolase fold superfamily.

Production and characterization of a thermostable extracellular esterase from Aspergillus niger

“…Tween-80 has the oleic acid composition in its nature and maintains the enzyme stability. As in literature it was reported that tween-80 helps the enzyme production and fungus growth when directly add into fermentation media (Castro et al, 2018). Addition of β-mercaptoethanol (1-3%v/v), disulfide bond-reducing agents, resulted in the stability of esterase activity, signifying that the enzyme did not have any important disulfide bond.…”

“…In the existance of anhydrous organic solvent system, esterases catalyze the esterification, transesterification, and interesterification. Moreover, they usually show enantioselective, chemioselective and regioselective properties (Castro et al, 2018).…”

Purification and Properties of an Esterase from Bacillus licheniformis and it’s Application in Synthesis of Octyl Acetate

“…In another study where prb A esterase was isolated from E. cloacae EM, the molecular mass of purified enzyme was found to be 55 kDa [24]. In a recent study, the molecular mass of purified esterase isolated from Aspergillus westerdijkiae was predicted to be 32 kDa [25]. Fig.…”

Producción de esterasas por microorganismos: importancia y aplicación industrial

“…Las esterasas se pueden producir de manera constitutiva o inducible (Gandolfi et al, 2000) y pueden ser excretadas por bacterias, levaduras u hongos como se muestra en la Tabla 1 donde se observa que Aspergillus westerdijkiae excreta serina esterasa y es considerada como un catalizador potencial ya que usa como sustrato a los ácidos grasos de cadena corta (Fernández-De Castro et al, 2017). Aspergillus fumigatus excreta glucuronoil esterasa la cual es importante para la degradación de la lignocelulosa (Huynh et al, 2018).…”