Production and characterization of a thermostable extracellular esterase from Aspergillus niger

Abstract

Modelado de la biodegradación en biorreactores de lodos de hidrocarburos totales del petróleo intemperizados en suelos y sedimentos (Biodegradation modeling of sludge bioreactors of total petroleum hydrocarbons weathering in soil and sediments)

Heterologous expression, molecular studies and biochemical characterization of a novel alkaline esterase gene fromBacillus thuringiensisfor detergent industry

“… 72 Reduced enzyme activity and stability reflects that histidine and serine may play a role in the enzyme's catalytic activity and substrate binding. Hamid et al 59 also reported the reduction in enzyme activity in the presence of SDS. Effect of organic solvents on enzyme's stability and activity was also determined in order for its utilization in industrial applications.…”

“…Various reports are present on the determination of thermostability of esterase enzyme from various microorganisms. Hamid et al 59 reported an esterase enzyme from Aspergillus niger with 56% activity at 80 °C after 4 h of incubation. While Tang et al 60 studied a glucuronoyl esterase from Thielavia terrestris and reported its thermostability at 55 °C.…”

Heterologous expression and characterization of a novel thermostable and alkali stable recombinant lipase enzyme from Bacillus thuringensis into E. coliBL21(DE3) for detergent formulation

“…Ca 2+ is reported to play important role in the structure and function of industrial enzymes (Haki & Rakshit, 2003). In previous studies, Ca 2+ stimulated lipases are reported for utilization in various industrial (Cherif et al, 2013; Hamid et al, 2021; Hertadi & Widhyastuti, 2015; Sayari et al, 2001). The addition of EDTA resulted in decrease of enzyme activity in this study and this confirms the fact that lipase is a metalloenzyme because EDTA is a strong inhibitor of metal dependent enzymes.…”

“…To evaluate the gene expression and molecular mass determination, SDS‐PAGE was used (Figure 2). Many reports are present on the use of SDS‐PAGE to measure the molecular mass of recombinant proteins (Basit et al, 2021; Gao et al, 2022; Hamid et al, 2021; Shariati et al, 2021; Zafar et al, 2022b).…”

Conditioning and use of prickly pear peels for the production of lignocellulosic enzymes by Aspergillus niger sp. on solid-state cultures

“…Hydrolases degrade polysaccharides of different lignocellulosic materials. Aspergillus species are the primary producers of several hydrolases, including esterases (Hamid et al, 2021), but they also produce enzymes that degrade lignin (Brink et al, 2019). The quantity and type of enzymes produced by fungi depend on the chemical composition of the material that they degrade (Costa et al, 2018, Martínez et al, 2017.…”