Production, purification, and characterization of a highly thermostable and humic acid biodegrading peroxidase from a decolorizing Streptomyces albidoflavus strain TN644 isolated from a Tunisian off-shore oil field

Characterization of a purified decolorizing detergent-stable peroxidase from Streptomyces griseosporeus SN9

“…The results showed that the bacterium S. griseosporeus strain SN9 was able to release a maximum peroxidase production yield of 0.172 U/ml at an initial alkaline medium pH (pH 8) after 5 days (Fig. 2E), which is in agreement with other reports on actinomycete-based peroxidase production [7,10]. However, the production of extracellular peroxidase by the S. griseosporeus strain SN9 was significantly decreased to 39% of the maximal production at pH 6.…”

“…2B, the optimum concentration of glucose for LiP-SN production was of 15 g/l (0.122 U/ml). Similar results were previously described in the literature, and glucose has often been reported to be necessary for peroxidase production [10,17].…”

“…Ten isolates were examined in the screening tests using o-dianisidine as a substrate. The same strains were inoculated in peroxidase screening media (PSM) (g/l): glucose, 4; malt extract, 10 …”

“…Kinetic parameters were calculated from the initial activity rates of the purified peroxidases, LiP-SN (this study) and HaP4 from S. albidoflavus strain TN644 [10], using 2,4-DCP and o-dianisidine as substrates. The pH and temperature values used in the kinetic study were adjusted to the optimum conditions for each enzyme (LiP-SN, pH 8.5, 65 • C; and HaP4, pH 4, 70 • C).…”

“…Moreover, and despite the large flow of data on peroxidases, only few studies have so far reported on the purification and characterization of peroxidases from Streptomyces [9][10][11][12][13]. Additionally, and to the authors' knowledge, no previous work has so far been performed to investigate the incorporation of peroxidase in detergent formulations.…”