Production of β-glucosidase byCladosporium resinae

We have developed a cellulose‐polyethylene blend polymer film for degradative susceptibility to brown rot or cellulasic fungi. Paxon low‐density polyethylene (LDPE) polymer pellets were melt‐blended with 8855 cellulose. A pure polyethylene sample was melted, pressed and used as the control. Naturally occurring fungi were harvested from decaying wood and cultured on full nutrient agar until individual phenotypes developed. The discrete fungal colonies were isolated and transferred to bacteriological agar with carboxymethylcellulose as the only carbon source. Surviving fungi were subjected to an indirect cellulase assay to confirm cellulolytic properties. Trichoderma viride showed 100% surface area growth on low‐density polyethylene (LDPE) blended with 8% (w/w) cellulose in 90 days. Growth coverage by Gliomastix on LDPE blended with 8% cellulose reached 100 % in 150 days, and up to 72% growth activity on blends with 1.5% cellulose, 1% cellulose, and the control LDPE.

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Biodegradation of ldpe/cellulose blends by common fungi

Foust

Mills

Haas

et al. 1997

Macromolecular Symposia

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Production and properties ofβ-glucosidase byNeurospora sitophila

Oguntimein

Moo‐Young

1991

World J Microbiol Biotechnol

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Growth at 25°C and pH 5.50 favour the production ofβ-glucosidase. De-fatted oilseed flour and Tween 80 enhanced the production ofβ-glucosidase, Lactose, gentibiose, gentibiose-acetate, laminarabiose and xylobiose inducedβ-glucosidase activity. Precipitation of the culture filtrate with (NH4)2SO4 resulted in 26-fold purification with 67% recovery. The optimum pH and temperature for activity were 5.0 to 5.4 and 55°C respectively. The enzyme was stable at 40°C with half-life at 12 h at 50°C. TheK m andV max for the hydrolysis ofp-nitrophenyl-β-D-glucoside at 40°C H 5.0 are 0.28MM and 0.60 U/mg protein, respectively.

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Isolation and Properties of an Extracellular β-Glucosidase from a Filamentous Fungus,Cladosporium resinae, Isolated from Kerosene

Hamada

Saito

et al. 1999

Bioscience, Biotechnology, and Biochemistry

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Production of β-glucosidase byCladosporium resinae

Cited by 3 publications

References 17 publications

Biodegradation of ldpe/cellulose blends by common fungi

Biodegradation of ldpe/cellulose blends by common fungi

Production and properties ofβ-glucosidase byNeurospora sitophila

Isolation and Properties of an Extracellular β-Glucosidase from a Filamentous Fungus,Cladosporium resinae, Isolated from Kerosene

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