Production of β-glucan exopolysaccharide lasiodiplodan by Lasiodiplodia theobromae CCT 3966 from corn bran acid hydrolysate

Philippini, Rafael R.; Martiniano, Sabrina Evelin; Marcelino, Paulo Ricardo Franco; Chandel, Anuj K.; Santos, Júlio César dos; Silva, Sílvio Silvério da

doi:10.1007/s00253-021-11173-6

Cited by 10 publications

(16 citation statements)

References 38 publications

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“…The study fulfilled by Philippini et al [13] revealed that the FTIR of glucan polymer lasiodiplodan produced by the strain L. theobromae CCT3966 from corn bran acid hydrolysate showed a β-type conformation. Similar to the current study, Kagimura et al [10] showed that the lasiodiplodan produced by L. theobromae MMPI had a structure with a very wide non-crystalline peak in the spectra 20-22 • (2θ).…”

Section: Discussionmentioning

confidence: 99%

“…XRD was conducted over a diffraction angle (2θ) of 10-60. X-ray diffractogram was attained by PANalytical (Malvern Panalytical, UK) [13].…”

Section: Scanning Electron Microscopy (Sem) Of Pretreated Biomassmentioning

confidence: 99%

“…The concentration of sugars was analyzed by High-Performance Liquid Chromatography (HPLC) in a Agilent 1200 series (Agilent Technologies Inc., USA) equipment with a Refractive index detector RID-6A and a HPX-87H (300 × 7.8 mm) column (Bio-Rad, USA). The analysis conditions were: column temperature of 45 • C, the solution of 0.01 N H 2 SO 4 as the mobile phase, flow rate of 0.6 mL/min, and 20 µL injection volume [13].…”

Section: Measurement Of Glucose In Hydrolysatementioning

confidence: 99%

“…Hence, production of lasiodiplodan from commercial sugars is not cost-efficient. In order to attain process economics and successful industrialization of lasiodiplodan production, the exploitation of abundant, renewable, and low-cost natural bioresources enhances economically feasible production of lasiodiplodan [13]. Considering these facts, there is a rising trend in the utilization of waste carbon sources for the production of biochemicals.…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, these raw materials are promising carbon sources and economically competitive ones as compared with food-based carbon sources [14,15]. However, current knowledge about lasiodiplodan synthesis from lignocellulosic biomass is rudimentary and very limited research has been performed in this regard [13,16].…”

Section: Introductionmentioning

confidence: 99%

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Fermentative Production of Lasiodiplodan by Lasiodiplodia theobromae CCT3966 from Pretreated Sugarcane Straw

et al. 2021

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Lasiodiplodan is a β-glucan polymer with different interesting characteristics, including therapeutic properties. It is an extracellular product, which is produced by the filamentous fungus Lasiodiplodia theobromae, using glucose as a substrate. In the present work, the production of lasiodiplodan was studied by the utilization of sugarcane straw as a low-cost carbon source. Glucose-rich sugarcane straw hydrolysate was obtained by a sequential pretreatment with dilute nitric acid (1% v/v) and sodium hydroxide (1% w/v), followed by enzymatic hydrolysis. The fermentation process was conducted by the cultivation of the strain Lasiodiplodia theobromae CCT3966 in sugarcane straw hydrolysate in a shake flask at 28 °C for 114 h. It was found that hydrolysate obtained after enzymatic hydrolysis contained 47.10 gL−1 of glucose. Fermentation experiments of lasiodiplodan synthesis showed that the peak yield and productivity of 0.054 gg−1 glucose consumed and 0.016 gL−1 h−1, respectively, were obtained at 72 h fermentation time. Fungal growth, glucose consumption, and lasiodiplodan production from sugarcane straw hydrolysate presented a similar pattern to kinetic models. The study on the chemical structure of lasiodiplodan produced showed it had a β-glucan construction. The current study revealed that sugarcane straw is a promising substrate for the production of lasiodiplodan.

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Section: Discussionmentioning

confidence: 99%

“…XRD was conducted over a diffraction angle (2θ) of 10-60. X-ray diffractogram was attained by PANalytical (Malvern Panalytical, UK) [13].…”

Section: Scanning Electron Microscopy (Sem) Of Pretreated Biomassmentioning

confidence: 99%

Section: Measurement Of Glucose In Hydrolysatementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%