Production of xylanases by Bacillus polymyxa using lignocellulosic wastes

Pham, Phuong Lan; Taillandier, Patricia; Delmas, M.; Strehaiano, Pierre

doi:10.1016/s0926-6690(97)00048-4

Cited by 29 publications

(11 citation statements)

References 16 publications

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“…After 6 days of incubation, amylase production decreased to 221.5 U/mL and 193.9 U/mL on the 7th and 8th day of incubation period, respectively. The observed decrease is most likely due to substrate inhibition [ 47 ]. Table 4 shows amylase production and supernatant protein concentration at different temperatures varying from 25°C to 50°C.…”

Section: Resultsmentioning

confidence: 99%

Production of Fungal Amylases Using Cheap, Readily Available Agriresidues, for Potential Application in Textile Industry

Singh

Bali

et al. 2014

BioMed Research International

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The study aimed at isolation and screening of fungal amylase producer, optimization of solid state fermentation conditions for maximum amylase production by the best amylase producer, and characterization of the crude amylases, so produced. Aspergillus fumigatus NTCC1222 showed the highest amylase activity (164.1 U/mL) in secondary screening under SSF conditions and was selected for further studies. The test strain showed maximum amylase production (341.7 U/mL) and supernatant protein concentration (9.7 mg/mL) for incubation period (6 days), temperature (35°C), initial pH (6.0), nutrient salt solution as moistening agent, and beef extract as nitrogen source. Pomegranate peel produced maximum amylase activity, but wheat bran (only slightly lesser amylase activity as compared to that of pomegranate peel) was chosen for further studies, keeping in mind the seasonal availability of pomegranate peel. TLC confirmed the amylase produced to be α-type and 60 kDa was the molecular weight of the partially purified amylase. The enzyme showed maximum enzyme activity at pH 6.0, temperature of 55°C, and incubation time of 60 minutes. UV (616.0 U/mL) and chemical (814.2 U/mL) mutation enhanced amylase activity as compared to wild test strain. The study indicates that Aspergillus fumigatus NTCC1222 can be an important source of amylase and the crude enzyme, hence obtained, can be cost effectively applied in multiple sections of textile wet processing.

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Section: Resultsmentioning

confidence: 99%

Production of Fungal Amylases Using Cheap, Readily Available Agriresidues, for Potential Application in Textile Industry

Singh

Bali

et al. 2014

BioMed Research International

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“…There were markedly decreases in activities at 60℃ and it took only three min to diminish xylanase activity to 50% of the initial activity. Xylanase from fungi and bacteria were often showed the optimum activity at 45~50℃, however it is usually inactivated above 60℃ (Lan-Phan et al, 1998). The stability against culture pHs are exhibited in Fig.…”

Section: Resultsmentioning

confidence: 99%

“…5). It suggests that decomposition products such as low molecular weight oligosaccharides existed in the rice bran contributes to the suppression of xylanase production in particular when high rice bran concentrations are tested (Lan-Phan et al, 1998; Mes-Hartree et al, 1998). As the accumulated hydrolysis products lead to repress enzyme production at higher substrate concentrations, the xylanase was also be possibly suppressed.…”

Section: Resultsmentioning

confidence: 99%

Production of Xylanolytic Enzyme Complex fromAspergillus flavususing Agricultural Wastes

Kim

2005

Mycobiology

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Five types of agricultural wastes were used for the production of xylanolytic enzyme by Aspergillus flavus K-03. All wastes materials supported high levels of xylanase and β-xylosidase production. A high level of proteolytic activity was observed in barley and rice bran cultures, while only a weak proteolytic activity was detected in corn cob, barley and rice straw cultures. Maximum production of xylanase was achieved in basal liquid medium containing rice barn as carbon source for 5 days of culture at pH 6.5 and 25℃. The xylanolytic enzyme of A. flavus K-03 showed low thermostability. The times required for 50% reduction of the initial enzyme activity were 90 min at 40℃, 13 min at 50℃, and 3 min at 60℃. Xylanolytic activity showed the highest level at pH 5.5~10.5 and more than 70% of the original activity was retained at pH 6.5 and 7.0. The higher stability of xylanolytic enzymes in the broad range of alkaline pH is useful for utilization of the enzymes in industrial process requiring in alkaline conditions. Moreover, the highest production of xylanolytic enzyme was obtained when 0.5% of rice bran was supplied in basal liquid medium. SDS-PAGE analysis revealed a single xylanase band of approximately 28.5 kDa from the culture filtrates.

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“…Among them, novel alginolytic activity in Paenibacillus , Leclercia and Planomicrobium has not been previously reported. Paenibacillus produces many kinds of extracellular enzymes such as cellulose, proteases, amylase and other polysaccharide-degrading enzymes, which can be used in a wide range of industrial fields (Adlakha et al 2015; Budi et al 2000; Das et al 2016; Dong et al 2016; Lan Pham et al 1998; Mathews et al 2016). However, production of alginate lyase by Paenibacillus has never been reported.…”

Section: Discussionmentioning

confidence: 99%

Screening of alginate lyase-excreting microorganisms from the surface of brown algae

et al. 2017

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Alginate lyase is a biocatalyst that degrades alginate to produce oligosaccharides, which have many bioactive functions and could be used as renewable biofuels. Here we report a simple and sensitive plate assay for screening alginate lyase-excreting microorganisms from brown algae. Brown algae Laminaria japonica, Sargassum horneri and Sargassum siliquatrum were cultured in sterile water. Bacteria growing on the surface of seaweeds were identified and their capacity of excreting alginate lyase was analyzed. A total of 196 strains were recovered from the three different algae samples and 12 different bacterial strains were identified capable of excreting alginate lyases. Sequence analysis of the 16S rRNA gene revealed that these alginate lyase-excreting strains belong to eight genera: Paenibacillus (4/12), Bacillus (2/12), Leclercia (1/12), Isoptericola (1/12), Planomicrobium (1/12), Pseudomonas (1/12), Lysinibacillus (1/12) and Sphingomonas (1/12). Further analysis showed that the LJ-3 strain (Bacillus halosaccharovorans) had the highest enzyme activity. To our best knowledge, this is the first report regarding alginate lyase-excreting strains in Paenibacillus, Planomicrobium and Leclercia. We believe that our method used in this study is relatively easy and reliable for large-scale screening of alginate lyase-excreting microorganisms.Electronic supplementary materialThe online version of this article (doi:10.1186/s13568-017-0361-x) contains supplementary material, which is available to authorized users.

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Production of xylanases by Bacillus polymyxa using lignocellulosic wastes

Cited by 29 publications

References 16 publications

Production of Fungal Amylases Using Cheap, Readily Available Agriresidues, for Potential Application in Textile Industry

Production of Fungal Amylases Using Cheap, Readily Available Agriresidues, for Potential Application in Textile Industry

Production of Xylanolytic Enzyme Complex fromAspergillus flavususing Agricultural Wastes

Screening of alginate lyase-excreting microorganisms from the surface of brown algae

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