Screening of alginate lyase-excreting microorganisms from the surface of brown algae

Wang, Mingpeng; Chen, Lei; Zhang, Zhaojie; Wang, Xuejiang; Qin, Song; Yan, Peisheng

doi:10.1186/s13568-017-0361-x

Cited by 29 publications

(19 citation statements)

References 38 publications

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“…We isolated strains affiliated with six rare actinobacterial genera – Rhodococcus , Nonomuraeae , Microbispora , Isoptericola , Nocardiopsis , and Microbacterium . Isolates from the latter three genera have also been obtained from macroalgae species, including some from the class Phaeophyceae (Hollants et al, 2013; Martin et al, 2014; Santhi et al, 2014; Wang et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

Actinobacteria Isolated From Laminaria ochroleuca: A Source of New Bioactive Compounds

Girão

Ribeiro

et al. 2019

Front. Microbiol.

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Nature is the major reservoir of biologically active molecules. The urgent need of finding novel molecules for pharmaceutical application is prompting the research of underexplored environments, such as marine ecosystems. Here, we investigated cultivable actinobacteria associated with the macroalgae Laminaria ochroleuca and assessed their potential to produce compounds with antimicrobial or anticancer activities. A specimen of L. ochroleuca was collected in a rocky shore in northern Portugal, and fragments of tissues from different parts of the macroalgae (holdfast, stipe, and blades) were surface sterilized and plated in three culture media selective for actinobacteria. A total of 90 actinobacterial strains were isolated, most of which affiliated with the genus Streptomyces . Isolates associated with the genera Isoptericola , Rhodococcus , Nonomuraeae , Nocardiopsis , Microbispora , and Microbacterium were also obtained. Organic extracts from the isolates were tested for their antimicrobial activity using the agar-based disk diffusion method, followed by determination of minimum inhibitory concentration (MIC) values. Forty-five isolates inhibited the growth of Candida albicans and/or Staphylococcus aureus , with MIC values ranging from <0.5 to 1000 μg mL −1 . The actinobacterial isolates were also tested for their anticancer potential on two human cancer cell lines. Twenty-eight extracts affected the viability of at least one human cancer cell line (breast carcinoma T-47D and neuroblastoma SH-SY5Y) and non-carcinogenic endothelial cell line (hCMEC/D3). Seven extracts affected the viability of cancer cells only. This study revealed that L. ochroleuca is a rich source of actinobacteria with promising antimicrobial and anticancer activities and suggests that macroalgae may be a valuable source of actinobacteria and, consequently, of new molecules with biotechnological importance.

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Section: Discussionmentioning

confidence: 99%

Actinobacteria Isolated From Laminaria ochroleuca: A Source of New Bioactive Compounds

Girão

Ribeiro

et al. 2019

Front. Microbiol.

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“…To establish seaweed as an effective biofertilizer, more in-depth studies are needed to determine the relationship between seaweed fertilizer use and the soil microbial community. In a previous study, we used the identified strain Bacillus litoralis to ferment Sargassum horneri and found that the fermented seaweed fertilizer with alginate oligosaccharides promoted root and seedling growth of tomato (Wang et al, 2016a(Wang et al, , 2017.…”

Section: Introductionmentioning

confidence: 99%

Responses of soil microbial communities to a short-term application of seaweed fertilizer revealed by deep amplicon sequencing

Wang

Chen

et al. 2018

Applied Soil Ecology

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Numerous studies have reported soil damage from chemical fertilizer application and an obvious promotional effect of seaweed fertilizer fermented with Sargassum horneri on the growth of tomato roots and seedlings due to its alginate oligosaccharide. However, few studies have assessed the effects of the fermented seaweed fertilizer on ecological environment and microorganisms in soil. Herein, our objective is to uncover microbial and soil environmental responses to Sargassum horneri-fermented seaweed fertilizer. After treated tomato-planting plots with Sargassum horneri fermented seaweed fertilizer, soil bacterial community compositions based on 16S rRNA gene amplicon sequencing, enzyme activities in soil and crop yield were analyzed. The bacterial α-diversity was strongly influenced by seaweed fertilizer amendment after 60 days. Non-metric multidimensional scaling (NMDS) analysis showed that a difference in bacterial community compositions between day 0 and day 60 was obvious for soil treated with seaweed fertilizer. The community variation could be caused by invertase activity and dehydrogenase activity in canonical correlation analysis (CCA). Protease activity, polyphenol oxidase activity and urease activity showed an obvious correlation with community variation in the Mantel test. The fertilization increased tomato yield by 1.48-1.83 times, Vc content by 1.24-4.55 times and lycopene content by 1.20-2.33 times. In the present study, a possible reason for bacterial community variation was discovered, which will provide an economical dilution rate of seaweed fertilizer for optimal crop yield and quality. Meanwhile, our study will be beneficial for developing a possible substitute for chemical fertilizer and an improved understanding of soil microbial functions and soil sustainability.

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“… Paenibacillus produces many kinds of extracellular enzymes, for instance, chitosanase [11], glucanase [12], multifunctional cellulase/mannanase/xylanase [13] and other enzymes, which can be used in a wide range of industrial fields. However, only several species in the genus were reported to possess alginate lyase [14–16]. Here we report the isolation and characterization of a novel alginate lyase-excreting species in the genus Paenibacillus , which was isolated from rotten brown alga samples from the South China Sea.…”

Section: Full-textmentioning

confidence: 98%

Paenibacillus algicola sp. nov., a novel alginate lyase-producing marine bacterium

Zhu

Zheng

et al. 2020

International Journal of Systematic and Evolutionary Microbiology

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A Gram-stain-variable, facultatively anaerobic, endospore-forming, rod-shaped bacterium, designated HB172198T, was isolated from brown alga collected at Qishui Bay, Hainan, PR China. Phylogenetic analysis of 16S rRNA gene sequences indicated that strain HB172198T belonged to the genus Paenibacillus , and the closest phylogenetically related species was Paenibacillus lemnae NBRC 109972T (97.6% similarity). The other 16S rRNA gene sequence similarities were under 97.0%. The whole genome average nucleotide identity value between strain HB172198T and the closest type strain was 75.3% and the in silico DNA–DNA hybridization value was 20.2%. The predominant isoprenoid quinone was menaquinone 7 and the major fatty acids were anteiso-C15:0, C16:0, anteiso-C17:0, iso C16:0 and C16:1 ω11c. The combined phylogenetic relatedness, phenotypic and genotypic features supported the conclusion that strain HB172198T represents a novel species of the genus Paenibacillus , for which the name Paenibacillus algicola sp. nov. is proposed. The type strain is HB172198T (=CGMCC 1.13583T=JCM 32683T).

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Screening of alginate lyase-excreting microorganisms from the surface of brown algae

Cited by 29 publications

References 38 publications

Actinobacteria Isolated From Laminaria ochroleuca: A Source of New Bioactive Compounds

Actinobacteria Isolated From Laminaria ochroleuca: A Source of New Bioactive Compounds

Responses of soil microbial communities to a short-term application of seaweed fertilizer revealed by deep amplicon sequencing

Paenibacillus algicola sp. nov., a novel alginate lyase-producing marine bacterium

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