Production of xylanase by immobilized Trichoderma reesei SAF3 in Ca-alginate beads

Abstract

In the present study, the optimum conditions for the production of xylanase by immobilized spores of Trichoderma reesei SAF3 in calcium alginate beads were determined. The operational stability of the beads during xylanase production under semi-continuous fermentation was also studied. The influence of alginate concentration (1, 2, 3, and 4%) and initial cell loading (100, 200, 300, 400, and 500 beads per flask) on xylanase production was considered. The production of xylanase was found to increase significantly with increasing concentration of alginate and reached a maximum yield of 3.12+/-0.18 U ml(-1) at 2% (w/v). The immobilized cells produced xylanase consistently up to 10 cycles and reached a maximum level at the forth cycle (3.36+/-0.2 U ml(-1)).

Enhanced production of a thermostable mannanase by immobilized cells of Bacillus subtilis on various membranes

“…Similarly, Dobreva et al (1998) reported that PS-immobilized Bacillus licheniformis cells showed a 58% increase of a-amylase production compared to the control after the fourth cycle of repeated batch cultivation. The reuse ability of the present immobilization system is in accordance with that reported in other findings (Dobreva et al 1998;Mamo and Gessesse 2000;Safarikova et al 2007;Anisha and Prema 2008;Kar et al 2008). …”

“…Cell immobilization is a promising method for enhancing production of extracellular enzymes. The use of immobilized cells offers several advantages over ordinary suspension culture systems (i.e., free cells), such as relative ease of enzyme purification and extraction step, higher yield of enzyme activity, higher operational stability, greater resistance to environmental perturbations and lower enzyme cost (Tonkova et al 1994;Mamo and Gessesse 2000;Konsoula and Liakopoulou-Kyriakides 2006;Safarikova et al 2007;Kapoor et al 2008;Kar et al 2008). Many different techniques for immobilizing cells such as adsorption, covalent linkage, gel entrapment or magnetic particles have been proposed during the last decades (Alekseiva et al 1998;Duran-Paramo et al 2000;Hashem et al 2001;Mohapatra et al 2007; Anisha and Prema 2008).…”

Enhanced production of alkaline thermostable keratinolytic protease from calcium alginate immobilized cells of thermoalkalophilic Bacillus halodurans JB 99 exhibiting dehairing activity

“…The alkaline protease production increased for ICLs up to 350 beads per flask and with increased stability. A similar observation was reported by Kar et al [16] who used 300 beads for xylanase production from Trichoderma reesei SAF3; Beshay [3] also reported 300 beads for maximum alkaline protease production from Teredinobacter turnirae; and Pradeep et al [19] showed maximum production of thermostable and neutral glycoamylase with 40 beads per 50 ml production medium using Ca-alginate immobilized Thermomucor indicae-seudaticae. During the present work, freely growing and immobilized cells followed similar fermentation profiles during batch cultivation.…”

Calcium alginate matrix increases the stability and recycling capability of immobilized endo-β-1,4-xylanase from Geobacillus stearothermophilus KIBGE-IB29

“…Selection of appropriate immobilization technique with support is also an important criterion in various bioprocesses. Due to non toxic property of calcium alginate, it is considered as a suitable matrix for the entrapment of biological macromolecules (Kar et al 2008). Alginate is a natural polysaccharide composed of 1,4 linked β-d mannuronic and α-l-guluronic acid residues, and in presence of calcium ions, alginate produces insoluble gel-like structure (calcium alginate) which is capable of tolerating high temperature and is biocompatible with most of the enzymes (Blandino et al 1999(Blandino et al , 2000.…”