Production of Wilbrandia ebracteata extract standardized in flavonoids and dihydrocurcubitacin and assessment of its topical anti-inflammatory activity

Peters, Carla Antunes; Sgrott, Roberta Aguiar Gomes; Peters, Rodrigo Rebelo; Moterle, Diego; Madeira, Fernanda Fernandez; Emer, Aline Armiliato; Piovezan, Anna Paula; Κanis, Luiz Alberto

doi:10.1016/j.indcrop.2015.01.018

Cited by 8 publications

(4 citation statements)

References 27 publications

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“…The skin irritation of the developed formulation was investigated in female mice to evaluate the safety of the formulation. 24 , 25 Twenty-four hours before the experiment, the hair on both sides of the spine (approximately 3 cm 2 ) was carefully removed with an electric razor without causing epidermal damage, and the mice were divided into three groups with six mice in each group. First group of mice received physiological saline and served as control, while second and third groups of mice received CUR solution and CUR-M, respectively.…”

Section: Methodsmentioning

confidence: 99%

Hyaluronic Acid Micelles for Promoting the Skin Permeation and Deposition of Curcumin

Niu¹,

Yuan²,

Zhang³

et al. 2022

IJN

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Background The poor skin permeation and deposition of topical therapeutic drugs is a major issue in topical drug delivery, improving this issue is conducive to improving the topical therapeutic effect of drugs. Methods In this study, octadecylamine modified hyaluronic acid (OHA) copolymer was synthesized by amide reaction technique to prepare curcumin (CUR)-loaded micelles (CUR-M) for topical transdermal administration. CUR-M was successfully prepared by dialysis, and the formulation was evaluated for particle size, zeta potential, surface morphology, entrapment effciency (EE%), drug loading (DL), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) and the in vitro drug release. Additionally, in vitro skin permeation and retention, in vivo topical analgesic and anti-inflammatory activity, and skin irritation were assessed. Results The mean drug loading (DL), drug entrapment efficiency (EE), hydrodynamic diameter and zeta potential of CUR-M were 8.26%, 90.86%, 165.64 nm and −26.85 mV, respectively. CUR-M was characterized by X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR), it was found that there was an interaction between CUR and OHA, and CUR existed in CUR-M in an amorphous form. CUR-M exhibited sustained release in 48 h and good stability at 4 °C for 21days. CUR-M could significantly increase the skin penetration and retention of CUR and had better analgesic and anti-inflammatory activities in vivo when compared with CUR solution. Hematoxylin-eosin staining results revealed that the transdermal penetration mechanism of CUR-M might be related to the hydration of stratum corneum by HA. In addition, CUR-M showed no skin irritation to mouse skin. Conclusion CUR-M might be a promising and safe drug delivery system for the treatment of topical diseases.

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Section: Methodsmentioning

confidence: 99%

Hyaluronic Acid Micelles for Promoting the Skin Permeation and Deposition of Curcumin

Niu¹,

Yuan²,

Zhang³

et al. 2022

IJN

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“…The dermal irritation/corrosion properties of the developed formulation were assessed in mice according to the methods previously described in the literature with some modifications. 38,39 Mice were anesthetized by intraperitoneal injection of 2% chloral hydrate (0.15 mL/10 g) and their two sides of the spinal (approximately 3 cm 2 ) were shaved 24 h before the test without causing tissue damage. Subsequently, filter papers (2 cm 2 ) were impregnated with 0.2 mL of formulation and then positioned on the dorsum of the mice, and fixed with hypersensitive tape.…”

Section: Acute Dermal Irritation Testmentioning

confidence: 99%

<p>Berberine-Loaded Thiolated Pluronic F127 Polymeric Micelles for Improving Skin Permeation and Retention</p>

Niu¹,

Yuan²,

Chen³

et al. 2020

IJN

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“…2 Acetic acid-induced vascular permeability in mice , COX and LOX enzymes and the inhibition of nitric oxide (NO) by modulating the inducible nitricoxide synthase (iNOS) enzyme [38].…”

Section: Croton Oil-induced Ear Edema In Micementioning

confidence: 99%

Antioxidant and Anti-Inflammatory Activities of Rubus Fruticosus and Zizyphus Vulgaris Methanol Extracts

Meziti

Bouriche

Hichem

et al. 2017

Int J Pharm Pharm Sci

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Objective: This report is an attempt to study the phenolic composition of Rubus fruticosus (RFE) and Zizyphus vulgaris (ZVE) methanol extracts and evaluate their antioxidant and anti-inflammatory effects in-vitro and in-vivo. Methods:Total phenolic and total flavonoids contents of extracts were determined by spectrophotometric methods. Phenolic compounds were identified by HPLC-TOF/MS. The antioxidant activities were evaluated in vitro using DPPH, ABTS and FRAP assays. The effect of RFE and ZVE on DNA cleavage induced by H2O2 Results:The phytochemical analysis of these extracts showed that RFE possesses higher polyphenolic and flavonoid content than ZVE. in the same way RFE exerted the highest antioxidant capacity with IC UV-photolysis was also investigated. The antioxidant effect of RFE and ZVE was tested in vivo using the blood total antioxidant capacity test in mice. On the other hand, the anti-inflammatory activity was assessed in vivo using two models of acute inflammation ear edema and vascular permeability. 50 value of 14 µg/ml in DPPH assay, 1.58 mmol of Trolox E/mg extract and 3.39 of mmol FesO4/mg extract in ABTS, and FRAP assay respectively. The studied extracts showed a concentration-dependent protective effect on DNA cleavage induced by H2O2 UV-photolysis. The daily oral administration of 200 mg/kg of RFE or ZVE during three weeks showed an improvement of the blood total antioxidant capacity; the HT50 Conclusion:The results obtained in this investigation showed that RFE possesses strong antioxidant and anti-inflammatory potential in comparison with ZVE, which may be attributed to the presence of polyphenolic phytoconstituents. values were151.45 min and 146.72 min for the groups treated with RFE and ZVE, respectively versus 122.5 min for the control group. The topical application of 2 mg/ear of RFE inhibited the croton oil-induced ear edema by 75.72%, while the inhibition exerted by ZVE was 64.24%. These inhibitions were higher than that of indomethacin, used as a reference. Moreover, the oral administration of 400 mg/kg of RFE inhibited significantly (33.57%) acetic acid induced vascular permeability in mice. However, this effect was lower than this of indomethacin. The inhibition effect exerted by ZVE was not significant.

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Production of Wilbrandia ebracteata extract standardized in flavonoids and dihydrocurcubitacin and assessment of its topical anti-inflammatory activity

Cited by 8 publications

References 27 publications

Hyaluronic Acid Micelles for Promoting the Skin Permeation and Deposition of Curcumin

Hyaluronic Acid Micelles for Promoting the Skin Permeation and Deposition of Curcumin

<p>Berberine-Loaded Thiolated Pluronic F127 Polymeric Micelles for Improving Skin Permeation and Retention</p>

Antioxidant and Anti-Inflammatory Activities of Rubus Fruticosus and Zizyphus Vulgaris Methanol Extracts

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