Production of transgenic indica rice resistant to yellow stem borer and sap-sucking insects, using super-binary vectors of Agrobacterium tumefaciens

Ramesh, S.; Nagadhara, D.; Reddy, V. D.; Rao, K. V.

doi:10.1016/j.plantsci.2003.12.028

Cited by 94 publications

(51 citation statements)

References 29 publications

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“…Cloning OsAP2/ERF in Agrobacterial T-DNA vector and transfer to Agrobacterium strain LBA4404 OsAP2/ERF full-length gene was PCR amplified by using a pair of primers, each containing a BamHI site and cloned into the BamHI site between CaMV35S promoter and PolyA on pSB11 binary vector (Ramesh et al 2004) (Supplementary Fig. S3).…”

Section: Sequence Analysis Of Osap2/erfmentioning

confidence: 99%

Transcriptional Profiling of Rice Leaves Undergoing a Hypersensitive Response Like Reaction Induced by Xanthomonas oryzae pv. oryzae Cellulase

Jha

Patel

Dasgupta

et al. 2009

Rice

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A secreted cellulase of Xanthomonas oryzae pv. oryzae induces innate immune responses in rice including a hypersensitive response (HR) like reaction. Microarray analysis was conducted using RNA isolated 12 h following ClsA treatment of leaves. BLAST searches were performed for the 267 (152 up-and 115 downregulated) differentially expressed (≥2-fold) genes. A number of defense and stressresponse functions are upregulated while a number of functions involved in metabolism and transport are downregulated following induction of HR. A significant proportion of the differentially expressed genes (41/267) are predicted to encode transcription factors. Co-infiltration of X. oryzae pv. oryzae suppresses ClsA-induced expression of two transcription factors, OsAP2/ethylene response factor (ERF) and OsRERJ1, that are predicted to be involved in the jasmonic-acid-mediated defense pathway. Transient transfer of OsAP2/ERF via Agrobacterium results in the induction of callose deposition, programmed cell death, and resistance against subsequent X. oryzae pv. oryzae infection.

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Section: Sequence Analysis Of Osap2/erfmentioning

confidence: 99%

Transcriptional Profiling of Rice Leaves Undergoing a Hypersensitive Response Like Reaction Induced by Xanthomonas oryzae pv. oryzae Cellulase

Jha

Patel

Dasgupta

et al. 2009

Rice

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“…Koul et al, 2014 Total larval mortality of 2 insect pests (Trichoplusia ni and Spodoptera exigua ) and high levels of plant protection have been observed on transgenic cotton plants expressing cry1Ac or cry1Ab genes from Bt var Kurstaki (HD-1 and HG-73, respectively). Perlak et al, 1990 Different cry toxins (cry1Ab, cry1ac or cry1B) have also been expressed in cereal crops like rice Cheng, Sardana, Kaplan, Altosaar 1998;Datta et al, 1998;Breitler 2001, Khanna, Raina 2002Ramesh, Nagadhara, Reddy, Rao 2004, maize Bohorova et al, 1999Armstrong 1995, Koziel et al, 1993and sorghum.Girijashankar et al, 2005 According to the reports, up to 100% larval mortality of the target insect pests as well as substantially reduced plant damage was observed on the transgenic plants of these crops.…”

Section: Insect Resistance Evaluation Of the Cry1ac Transgenic Pea Linesmentioning

confidence: 99%

Molecular and functional characterization ofcry1Actransgenic pea lines

et al. 2016

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ABSTRACT. Transgenic pea lines transformed with the cry1Ac gene were characterized at molecular (PCR, RT-PCR, qRT-PCR and immunostrip assay) and functional levels (leaf paint and insect feeding bioassays). The results showed the presence, expression, inheritance and functionality of the introduced transgene at different progeny levels. Variation in the expression of the cry1Ac gene was observed among the different transgenic lines. In the insect bioassay studies using the larvae of Heliothis virescens, both larval survival and plant damage were highly affected on the different transgenic plants. Up to 100 % larval mortality was observed on the transgenic plants compared to 17.42 % on control plants. Most of the challenged transgenic plants showed very negligible to substantially reduced feeding damage indicating the insect resistance of the developed transgenic lines. Further analysis under field condition will be required to select promising lines for future uses.

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“…The rice plants were artificially infested ( Figures 1A-1C) with the target insects and their attack was measured quantitatively. Some transgenic rice lines/ varieties resistant to YSB have also been developed in India (Ramesh et al, 2004). Transgenic rice plants have also been developed in China and their efficacy was tested in the laboratory as well as in the field (Wang et al, 2012;Li et al, 2013;Li F et al, 2014).…”

Section: Insect-resistant Ricementioning

confidence: 99%

Genetic improvement of rice for biotic and abiotic stress tolerance

Ansari¹,

Shaheen²,

Bukhari³

et al. 2015

Turk J Bot

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IntroductionRice (Oryza sativa L.) is one of the oldest cultivated crops. It has been cultivated in India and China for several thousands of years (Poehlman and Sleper, 1995). The major cultivated species of rice, Oryza sativa (2n = 2x = 24), originated in southern and southwestern tropical Asia. The other species of cultivated rice, Oryza glaberrima (2n = 2x = 24), is indigenous to the upper valley of the Niger River and it is cultivated only in western tropical Africa. Twenty-three species and 10 recognized genome types (AA, BB, CC, BBCC, CCDD, EE, FF, GG, HHJJ, and HHKK; Gramene: http://www.gramene.org/species/ oryza/rice_taxonomy.html) of Oryza are recognized. Close relatives of O. sativa are the wild perennial species O. rufipogon and the wild annual species O. nivara. Both are diploid weedy species with the AA genome.Rice is an important food crop and it needs continuous improvement due to the continuous increase in population. The major objectives to improve rice crops using biotechnological techniques include: 1) high yield potential, 2) early maturity, 3) resistance to lodging and shattering, 4) resistance to stress environments, 5) disease resistance, 6) insect resistance, 7) grain quality, and 8) enhancement of nutritional components. Economic importance of riceRice (Oryza sativa L.) is the most important crop of world. About 90% of the world's rice is grown in China, India, Pakistan, Japan, Korea, Southeast Asia, and other adjacent areas (USDA, 2014). Outside of Asia, Brazil and the United States produce the largest amounts of rice (Poehlman and Sleper, 1995). Rice is the staple food for over one-half of the world's people (FAO, 2008). In Pakistan, rice is a highly valued food crop and it is also a major export item. It accounts for 3.2% of the total value added in agriculture and 0.7% of the GDP. Area sown for rice is estimated at 2.891 × 10 6 ha and the production was 7.005 × 10 6 t in -2015 (Ministry of Finance, 2015. Rice is cultivated in diversified climatic conditions of Pakistan. Basmati rice (Indica) is grown in the traditional rice-growing belt of Punjab Province. In Swat, in high altitude alpine valleys, temperate Japonica rice is grown. In the south of Khyber Pakhtunkhwa, Sindh and Baluchistan provinces, IRRItype long-grain heat-tolerant tropical rice is mainly grown. Transformation of rice cropsThe world will need about 25% more rice by the year 2030 to meet the estimated demand of an increasing global population (Wani and Sah, 2014). One way to meet this challenge is to grow rice on more area, which is difficult due to increasing urbanization and escalating population in Abstract: Rice (Oryza sativa L.) is among the most important food crops that provide a staple food for nearly half of the world's population. Rice crops are prone to various types of stresses, both biotic and abiotic. Biotic stresses include insect pests, fungus, bacteria, viruses, and herbicide toxicity. Among abiotic stresses, drought, cold, and salinity are also well studied in rice. Various genes have been identified,...

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Production of transgenic indica rice resistant to yellow stem borer and sap-sucking insects, using super-binary vectors of Agrobacterium tumefaciens

Cited by 94 publications

References 29 publications

Transcriptional Profiling of Rice Leaves Undergoing a Hypersensitive Response Like Reaction Induced by Xanthomonas oryzae pv. oryzae Cellulase

Transcriptional Profiling of Rice Leaves Undergoing a Hypersensitive Response Like Reaction Induced by Xanthomonas oryzae pv. oryzae Cellulase

Molecular and functional characterization ofcry1Actransgenic pea lines

Genetic improvement of rice for biotic and abiotic stress tolerance

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