Transcriptional Profiling of Rice Leaves Undergoing a Hypersensitive Response Like Reaction Induced by Xanthomonas oryzae pv. oryzae Cellulase

Abstract

A secreted cellulase of Xanthomonas oryzae pv. oryzae induces innate immune responses in rice including a hypersensitive response (HR) like reaction. Microarray analysis was conducted using RNA isolated 12 h following ClsA treatment of leaves. BLAST searches were performed for the 267 (152 up-and 115 downregulated) differentially expressed (≥2-fold) genes. A number of defense and stressresponse functions are upregulated while a number of functions involved in metabolism and transport are downregulated following induction of HR. A significant proportion of the differentially expressed genes (41/267) are predicted to encode transcription factors. Co-infiltration of X. oryzae pv. oryzae suppresses ClsA-induced expression of two transcription factors, OsAP2/ethylene response factor (ERF) and OsRERJ1, that are predicted to be involved in the jasmonic-acid-mediated defense pathway. Transient transfer of OsAP2/ERF via Agrobacterium results in the induction of callose deposition, programmed cell death, and resistance against subsequent X. oryzae pv. oryzae infection.

Upregulation of jasmonate biosynthesis and jasmonate-responsive genes in rice leaves in response to a bacterial pathogen mimic

“…Twenty-five to 30 leaf pieces covering the infiltrated zone from each treatment, LipA or buffer (10 mM potassium phosphate buffer, pH 6.6), were harvested 12 h after infiltration. RNA was isolated from each treatment and quality was assessed as described previously (Jha et al 2010). Eight micrograms of total RNA isolated from each treatment (LipA or buffer) was used for Affymetrix microarray hybridization.…”

“…Comparative analysis of rice innate immune responses induced by the cell wall degrading enzymes ClsA and LipA Rice gene expression changes following treatment with the cell wall degrading enzyme, ClsA, have been determined (Jha et al 2010). In order to compare the ClsA-and LipA-induced transcriptional changes in rice leaves, we have reanalyzed the ClsA-induced transcriptome data by retrieving the CEL files from the NCBI microarray repository, Gene Expression Omnibus (GEO) (http://www.ncbi.nlm.nih.gov/geo), accession number GSE8216.…”

“…Taken together, this suggests a possible role of JA-Ile in LipA-induced rice defense responses. Comparative analysis of gene expression changes induced by cell wall degrading enzymes, ClsA and LipA Jha et al (2010) have analyzed the rice gene expression changes that are induced following treatment with a cellulase (ClsA). Prior treatment with either ClsA or LipA results in enhanced resistance against subsequent infection by X. oryzae pv.…”

Transcriptional dynamics during cell wall removal and regeneration reveals key genes involved in cell wall development in rice

“…The data was compared with the genes induced in response to sucrose starvation in rice suspension cells (Wang et al 2007) and cellulase treatment to rice leaves (Jha et al 2010). Microarray analysis has also been reported to identify genes induced during cell elongation (Kwon et al 2005) and cell wall regeneration using suspension cells in Arabidopsis (Irshad et al 2008).…”

“…The datasets used for comparison include sucrose starvation in suspension cells (Wang et al 2007); cellulase treatment to leaves (Jha et al 2010); Xanthomonas oryzae pv. oryzae (XOO) and Xanthomonas oryzae pv.…”