Production of the secondary metabolite catechin by in vitro cultures of Camellia sinensis L

Abstract

BackgroundCatechin is one of the secondary metabolites in Camellia sinensis L. that is alternatively produced through in vitro cultures. The in vitro culture product is possibly improved by optimizing the culture medium with the addition of growth regulators and precursors. The purpose of this study was to confirm the success of the secondary catechin metabolite production through the in vitro culture of C. sinensis L in a relatively short time.MethodsThe secondary catechin metabolite product is obtained in about 40 days. The study was conducted by (1) leaf cutting for inoculation in Murashige and Skoog media with 1 μg/mL of 2,4-dichlorophenoxyacetic acid growth regulator; (2) the inoculation of callus multiplication on the same medium as a partially modified inoculation media condition with the addition of 1 μg/mL of 6-benzylaminopurine (BAP) and 2 μg/mL of 2,4-dichlorophenoxyacetic acid at concentration; (3) callus multiplication developed on a new medium containing phenylalanine precursors (300 μg/mL); (4) testing growth by harvesting the callus and weighing the wet weight of its biomass and (5) identification of the callus qualitatively and quantitatively by using high-performance liquid chromatography (HPLC).ResultsThe level of secondary catechin metabolite produced was 2.54 μg/mL and 12.13 μg/mL in solid and suspension media, respectively.ConclusionsIt is concluded that the method is effective and efficient in producing catechin product from C. sinensis L.

The Influence of Exogenous Phenylalanine on the Accumulation of Secondary Metabolites in Agitated Shoot Cultures of Ruta graveolens L.

“…For example, in a study on cell suspension cultures of Ginkgo biloba , an increase in the production of phenolic acids was observed after the administration of 0.66–1.33 g/L of phenylalanine [ 14 ]. In turn, in a study on Camellia sinensis callus cultures, the addition of 0.3 g/L of phenylalanine resulted in increased production of catechins [ 37 ]. In a study on Rhodiola imbricata cell suspension cultures, the addition of phenylalanine at different concentrations (0.5–3 mM) caused an increase in the production of rosavin, rosarin, and p -coumaric acid [ 38 ].…”

Production of secondary metabolites using tissue culture-based biotechnological applications

“…Examples of secondary metabolites obtained using suspension cultures can be given as stigmasterol from Abutilon indicum ( Rao et al., 2021 ); gymnemic acids from Gymnema sylvestre ( Mahendran et al., 2021 ); catechin from Camellia sinensis ( Ardianto et al., 2020 ); alkaloids (vincristine, vinblastine, ajmalicine and serpentine) from Catharanthus roseus ( Mishra et al., 2019 ); triterpenoids from Ocimum basilicum ( Pandey et al., 2019 ); artemisinin from Artemisia annua ( Mir et al., 2017 ); plumbagin from Plumbago europaea ( Beigmohamadi et al., 2019 ) and P. zeylanica ( Roy and Bharadvaja, 2019 ); bacoside from Bacopa monnieri ( Kharde et al., 2018 ); hydrolyzable tannin from Phyllanthus debilis ( Malayaman et al., 2017 ); triterpenoids from Euphorbia hirta ( Samkumar et al., 2019 ); atropine from Hyoscyamus muticus ( Abdelazeez et al., 2022 ); triterpenic acids (betulinic acid, oleanolic acid, and ursolic acid) from Thymus persicus (Lamiaceae) ( Bakhtiar and Mirjalili, 2022 ); and withanolides (withaferin A and withanolide A) from Withania coagulans ( Mirjalili and Esmaeili, 2022 ).…”

The Role of in Vitro Culture Producing Secondary Metabolites and Plant Conservation Techniques to Overcome the Environmental Crisis

“…Quinine aromatic bioactive compound obtained from Aloe vera plant culture functioned as a cosmetic and laxative (Wang et al, 2017). The secondary metabolite biomass of catechins has been successfully extracted from in vitro culture of Camellia sinensis as a candidate for anti-inflammatory medicinal raw materials (Sutini et al, 2020).…”