Production of shikonin derivatives by cell suspension cultures of Lithospermum erythrorhizon. III. Comparison of shikonin derivatives of cultured cells and ko-shikon

Abstract

A method for quantitative analysis of shikonin derivatives using high pressure liquid chromatography (HPLC) was established. With this method the composition of shikonin derivatives in cultured cells and roots of Lithospermum erythrorhizon (ko-shikon) was compared. The composition of shikonin derivatives produced by cell suspension cultures was similar to that of the ko-shikon, and the composition in cultured cells was found to fluctuate less than that of the ko-shikon.

Large-Scale Production of Rosmarinic Acid from Plant Cell Cultures of Coleus blumei Benth.

“…erythrorhizon Sieb. et Zucco cell culture using a two-stage procedure: mass cultivation of plant cells in growth medium with subsequent transfer of the cells into a special production medium [9][10][11][12].…”

An envelope-shaped film culture vessel for plant cell suspension cultures and metabolite production without agitation

“…TLC analysis was carried out using precoated silica gel TLC (Merck) with CHCI3 as a solvent system. HPLC was carried out according to the method of Fujita et al (1983) with a minor modification; column: TSK-GEL ODS 120A, 10 ~tm (Toyo Soda, Japan), 150 × 4.6 mm, solvent system: CH3CN -H20 -AcOH -Et3N (70 : 30 : 0.3 : 0.3), flow rate: 1 ml min -1, detection: absorbance at 520 nm. The quantities of shikonin derivatives were estimated as shikonin from the corresponding areas recorded by Chromatopac C-R6A (Shimadzu).…”

“…The red pigments in the cultured cells of Lithospermum erythrorhizon consists of several kinds of shikonin derivatives (Fujita et al 1983). HPLC analysis (Fig.…”

Shikonin: Production by Plant (Lithospermum erythrorhizon) Cell Cultures

“…Furthermore, only three of the eight derivatives can be separated. Fujita et al (1983) used high pressure liquid chromatography (HPLC) because shikonin derivatives are not stable at high temperatures and their molecular weight ranges from 256 to 388.…”