Production of second generation rabbit embryos by nuclear transfer

Yin, Xi-Jun; Choi, Chang-Rok; Kong, Il‐Keun; Choe, Sang-Yong; Park, C.S.; Lee, H.J.

doi:10.1016/s0093-691x(98)90684-7

Cited by 4 publications

(2 citation statements)

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“…The second series of cloning by this method supplied 35.7% of blastocysts [14]. Blastocyst formation in the preactivated group shown in this paper suggests that electric field preactivation is even more effective (56% vs. 15%).…”

Section: Preimplantation Development Of Nt Rabbit Embryos In Vitromentioning

confidence: 69%

“…In this trial, oocytes recovered 17-18 h after hCG treatment were enucleated and activated and fused with 1/8 blastomeres by applying three DC pulses of 2-2.5 kV/cm for 50-70 sec each [5]. The yield of about 15% of blastocysts remains almost a standard in subsequent trials that applied similar experimental parameters ( [14], also the untreated group in the present paper). Applying an alternating current (AC) pulse of 0.1 kV/cm for 5-10 sec followed by two DC pulses of 2.4 kV/cm for 60 sec with an interval of 30 min.…”

Section: Preimplantation Development Of Nt Rabbit Embryos In Vitromentioning

confidence: 92%

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Effects of Preactivation of Ooplasts or Synchronization of Blastomere Nuclei in G1 on Preimplantation Development of Rabbit Serial Nuclear Transfer Embryos1

Piotrowska

Modliński

Korwin-Kossakowski

et al. 2000

Biology of Reproduction

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Blastomeres from eight-cell-stage rabbit embryos have been fused with enucleated metaphase II oocytes (ooplasts) or with ooplasts that were preactivated before fusion. Preactivation of ooplasts before nuclear transfer (NT) raises the rate of preimplantation development from 15% to 56%, which remains elevated in the next series of NT (48.6% and 47.2% in the second and third rounds, respectively). Transfer of eight-cell embryos from the third round to the recipient resulted in the birth of normal young. Synchronization of blastomere nuclei in the G1 phase with nocodazole before fusion results in 42% morula/blastocyst formation. However, in the second generation of NT embryos, the yield drops to as low as 17%, indicating deleterious effects of the second nocodazole treatment on blastomeres. The calculated number of clones per one round of cloning was 4.5, 3.9, and 3.8 in subsequent series; the highest number of morulae and blastocysts that developed from individual donor embryos after three rounds were 26 and 27, respectively.

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Section: Preimplantation Development Of Nt Rabbit Embryos In Vitromentioning

confidence: 69%

Section: Preimplantation Development Of Nt Rabbit Embryos In Vitromentioning

confidence: 92%

Effects of Preactivation of Ooplasts or Synchronization of Blastomere Nuclei in G1 on Preimplantation Development of Rabbit Serial Nuclear Transfer Embryos1

Piotrowska

Modliński

Korwin-Kossakowski

et al. 2000

Biology of Reproduction

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Effect of donor cell age on the efficiency of nuclear transfer in rabbits

Galat¹,

Lagutina²,

Mezina³

et al. 2002

Reproductive BioMedicine Online

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The ability of rabbit fibroblasts of different ages to be reprogrammed following nuclear transfer (NT) to aged recipient oocytes was evaluated. The rate of NT blastocysts reconstructed with presumptive G1 stage morula cells or fetal fibroblasts was significantly higher (41.5% and 51.4%) than was those of cloned embryos reconstructed with fibroblasts from young (4-month-old) or aged (5-year-old) animals (16.7% and 7.1%, respectively, P < 0.025). Serum starvation significantly increased the development of NT embryos to the morula-blastocyst stage (67.6% versus 22.9%, P < 0.025). Transfer of 168 NT embryos derived from nuclei of morula cells and 106 control embryos into 21 recipients resulted in 10 pregnancies, 2 NT and 18 control pups, respectively. In the first experiment, transfer of 142 cleaved NT embryos reconstructed with fetal fibroblasts and 86 control embryos into eight recipient does resulted in five pregnancies and the birth of 20 control pups. In the second experiment, after transfer of 112 NT embryos derived from fetal fibroblasts into six recipients, 10 (8.9%) sites of implantation were revealed in two does (33.3%) on day 14 of gestation. This study provides evidence that nuclei of morula cells and fetal and adult fibroblasts differ in their ability to be reprogrammed by recipient cytoplasm following nuclear transfer.

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