Production of Potent Antimicrobial Compounds from Streptomyces cyaneofuscatus Associated with Fresh Water Sediment

Zothanpuia,; Passari, Ajit Kumar; Chandra, Preeti; Leo, Vincent Vineeth; Mishra, Vineet Kumar; Kumar, Brijesh; Singh, Bhim Pratap

doi:10.3389/fmicb.2017.00068

Cited by 51 publications

(52 citation statements)

References 48 publications

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“…However, extensive use of antibiotics has led to the evolution of antibiotics resistance in many bacterial species. Despite the success of the discovery of new drugs to fight against antibiotic resistance, bacterial infections remain the top leading cause of death throughout the world in 2015 [ 25 , 26 ]. Thus, there is an urgent need for the discovery of promising new antimicrobial compounds.…”

Section: Introductionmentioning

confidence: 99%

Anti-BACE1 and Antimicrobial Activities of Steroidal Compounds Isolated from Marine Urechis unicinctus

Zhu

Wang

et al. 2018

Marine Drugs

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The human β-site amyloid cleaving enzyme (BACE1) has been considered as an effective drug target for treatment of Alzheimer’s disease (AD). In this study, Urechis unicinctus (U. unicinctus), which is a Far East specialty food known as innkeeper worm, ethanol extract was studied by bioassay-directed fractionation and isolation to examine its potential β-site amyloid cleaving enzyme inhibitory and antimicrobial activity. The following compounds were characterized: hecogenin, cholest-4-en-3-one, cholesta-4,6-dien-3-ol, and hurgadacin. These compounds were identified by their mass spectrometry, 1H, and 13C NMR spectral data, comparing those data with NIST/EPA/NIH Mass spectral database (NIST11) and published values. Hecogenin and cholest-4-en-3-one showed significant inhibitory activity against BACE1 with EC50 values of 116.3 and 390.6 µM, respectively. Cholesta-4,6-dien-3-ol and hurgadacin showed broad spectrum antimicrobial activity, particularly strongly against Escherichia coli (E. coli), Salmonella enterica (S. enterica), Pasteurella multocida (P. multocida), and Physalospora piricola (P. piricola), with minimal inhibitory concentration (MIC) ranging from 0.46 to 0.94 mg/mL. This is the first report regarding those four known compounds that were isolated from U. unicinctus and their anti-BACE1 and antimicrobial activity, highlighting the fact that known natural compounds may be a critical source of new medicine leads. These findings provide scientific evidence for potential application of those bioactive compounds for the development of AD drugs and antimicrobial agents.

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Section: Introductionmentioning

confidence: 99%

Anti-BACE1 and Antimicrobial Activities of Steroidal Compounds Isolated from Marine Urechis unicinctus

Zhu

Wang

et al. 2018

Marine Drugs

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“…A closer look at genus level specifically within the Actinobacterial community highlighted eight predominant genera namely Acidothermus (29.6%), Actinospica (17.65%), Streptomyces (14.64%), Nocardia (4.55%), Sinomonas (2.90%), Catenulispora (2.57%) and Geodermatophilus (1.6%) dominated in varying proportions throughout the study period ( Figure 2B ). This is in sharp contrast to freshwater river, lake and agricultural pond being dominated by Actinomycetales and Streptomyces [17, 10]. Although Streptomyces was not the most dominated in this study, it occupied the 3 rd most abundant genus within Actinobacterial phylum indicating its predominant nature.…”

Section: Discussionmentioning

confidence: 60%

“…It has been suggested that members of the rare biosphere may play important roles in the bacterial community [22, 28]. Freshwater ecosystem, especially stagnant water bodies such as lakes have shown some promising results for rare Actinobacterial genus [10, 17]. Development of new selective techniques and expression based metagenomics would enable screening, isolation, and discoveries of rare antibiotic genes which could lead to useful bioactive compounds.…”

Section: Discussionmentioning

confidence: 99%

Metagenomic Insights Unveil the Dominance of Undescribed Actinobacteria in Pond Ecosystem of an Indian Shrine

Radhakrishnan

Imchen

Kaari

et al. 2018

Preprint

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44Metagenomic analysis holds immense potential for identifying rare and uncharacterized 45 microorganisms from many ecological habitats. Actinobacteria have been proved to be an 46 excellent source of novel antibiotics for several decades. The present study was designed to 47 delineate and understand the bacterial diversity with special focus on Actinobacteria from pond 48 sediment collected from Sanjeeviraya Hanuman Temple, Ayyangarkulam, Kanchipuram, Tamil 49 Nadu, India. The sediment had an average temperature (25.32%), pH (7.13), salinity (0.960 50 mmhos/cm) and high organic content (10.7%) posing minimal stress on growth condition of the 51 microbial community. Subsequent molecular manipulations, sequencing and bioinformatics 52 analysis of V3 and V4 region of 16S rRNA metagenomics analysis confirmed the presence of 40 53 phyla, 100 classes, 223 orders, 319 families and 308 genera in the sediment sample dominated by 54 56(1.63%). Among the Actinobacteria phylum, Acidothermus (29.68%) was the predominant genus 57 followed by Actinospica (17.65%), Streptomyces (14.64%), Nocardia (4.55%) and Sinomonas 58 (2.9%). Culture-dependent isolation of Actinobacteria yielded all strains of similar morphology 59 to that of Streptomyces genus which clearly indicating that the traditional based technique is 60 incapable of isolating majority of the non-Streptomyces or the so called rare Actinobacteria. 61 Although Actinobacteria were among the dominant phylum, a close look at the species level 62 indicated that only 15.2% within the Actinobacterial phylum could be assigned to cultured 63 species. This leaves a vast majority of the Actinobacterial species yet to be explored with 64 possible novel metabolites have special pharmaceutical and industrial application. It also 65 indicates that the microbial ecology of pond sediment is neglected fields which need attention. 66 67 metabolites 68 It is estimated that only 1% of microbes in the natural environment are culturable under 70 laboratory conditions [46]. More than 88% of culturable isolates belong to four phyla known as 71 Proteobacteria, Firmicutes, Actinobacteria, and Bacteroidetes [47]. However, uncultivated 72 bacteria possess a great diversity of enzymes adaptable to different environmental conditions 73 which could serve as a prolific source of novel bioactive compounds with industrial and 74 pharmaceutical applications. Metagenomics is a tool to study massive microbial communities 75 prevailing in the environment in its natural state irrespective of its uncultivable properties. This 76technique has not only helped to overcome the barriers of culture dependent techniques but also 77 identified rare microorganisms [23]. It also provides a wide and unbiased view of the potential 78 microbial functions in a single snapshot with high resolution contributing immensely to the 79 global microbial diversity studies from different ecosystem and their potential ecological 80 functions [27]. On the other hand, there have been several reports on the widespread distribution...

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“…Recent findings showed presence of four volatile compounds which might be responsible for diverse biological activity of the secondary metabolites from Streptomyces sp. (Zothanpuia et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

Morphological, Biochemical and Molecular Characterization of Culturable Epilithic and Endolithic Bacteria From Rocks of Ayubia (Murree), Lower Himalaya, Pakistan

Khan¹,

Sajjad²,

Zada³

et al. 2018

Appl. Ecol. Env. Res.

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Abstract. Murree and Ayubia hills are parts of the lower Himalaya Mountain range in Pakistan. The rocks of these hills are younger and mostly limestone in nature. In this study, geomicrobiological investigation of the selected samples collected from Murree and Ayubia hills was carried out. The rock samples were spread on nutrient agar plates and endolithic and epilithic bacteria were isolated on the basis of colony morphology and studied further. All the isolates were screened for different industrially important hydrolytic enzymes. Study isolates were identified through 16S rRNA gene sequencing. These endoliths and epiliths were assayed for antimicrobial activities against clinical isolates S. aureus, P. aeuroginosa and E. coli. Total 31 bacterial isolates were recovered of which 15 were endolithic and 16 were epilithic. 16S rRNA gene sequencing revealed major culturable groups colonizing these rocks were clustered in four major groups, Proteobacteria, Firmicutes, Actinobacteria and Bacteroidetes which inlcude Alcaligenes, Lysinibacillus, Actinobacteria, Pseudomonas, Pusillimonas, Streptomyces, Fluviicola, Serratia, Flavobacterium, Stenotrophomonas and Brevindomonas species. The sequences were deposited in NCBI for acquisition of accession numbers. The bacterial isolates were efficient producers of oxidase, catalase, protease, amylase and gelatinase. The endolithic isolates N4 (Pseudomonas sp. KT223616) and N28 (Streptomyces sp. KT004386) exhibited good activity against all the three clinically isolated target strains. The study revealed the rocks of Murree and Ayubia hills have a rich microbial ecology that besides having an important role in weathering and mineralization processes may also be potential source of biotechnological applications.

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Production of Potent Antimicrobial Compounds from Streptomyces cyaneofuscatus Associated with Fresh Water Sediment

Cited by 51 publications

References 48 publications

Anti-BACE1 and Antimicrobial Activities of Steroidal Compounds Isolated from Marine Urechis unicinctus

Anti-BACE1 and Antimicrobial Activities of Steroidal Compounds Isolated from Marine Urechis unicinctus

Metagenomic Insights Unveil the Dominance of Undescribed Actinobacteria in Pond Ecosystem of an Indian Shrine

Morphological, Biochemical and Molecular Characterization of Culturable Epilithic and Endolithic Bacteria From Rocks of Ayubia (Murree), Lower Himalaya, Pakistan

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