Production of Polyhydroxybutyrate (PHB) from Streptomyces Incanus and the Effect of Gamma Irradiation on its Production

Rezk, Samar Abdelfattah; Emam, Doaa A.; Swailam, Hesham M.; Swelim, M. A.

doi:10.21608/ajnsa.2020.16698.1266

Cited by 3 publications

(4 citation statements)

References 11 publications

(14 reference statements)

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“…Species of the genus Bacillus have the ability to utilize these diverse and cheap carbon wastes, as they possess hydrolytic enzymes capable of metabolizing these complex residues. Different agricultural waste materials (wheat bran, rice bran, rice straw, and molasses) were employed as the main carbon source for PHB production by Streptomyces incanus [14], and wheat bran has the highest PHB production of 2.82 g/L. In this study, the carbon source was replaced by the extracts of five different agricultural waste materials including orange peel, banana peel, mango peel, onion peel, and rice straw for PHB production.…”

Section: Production Of Phb From Waste Materialsmentioning

confidence: 99%

“…Mostafa and his coworkers [13] isolated novel strains of PHB-producing bacteria (Tamlana crocina, Bacillus aquimaris, Erythrobacter aquimaris, and Halomonas halophila) from the mangrove rhizosphere, Red Sea, Saudi Arabia, which can make PHB production a decent contender for numerous industrial applications as a substitution for petroleum-based plastics. Rezk et al (2020) [14] proved that wheat bran can be used as an alternative to starch nitrate medium for PHB production by Streptomyces incanus BK128.…”

Section: Introductionmentioning

confidence: 99%

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Bioplastic Production by Bacillus wiedmannii AS-02 OK576278 Using Different Agricultural Wastes

et al. 2021

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Polyhydroxybutyrates (PHBs) are macromolecules synthesized by bacteria. Because of their fast degradability under natural environmental conditions, PHBs were selected as alternatives for the production of biodegradable plastics. Sixteen PHB-accumulating strains were selected and compared for their ability to accumulate PHB granules inside their cells. Isolate AS-02 was isolated from cattle manure and identified as Bacillus wiedmannii AS-02 OK576278 by means of 16S rRNA analysis. It was found to be the best producer. The optimum pH, temperature, and incubation period for the best PHB production by the isolate were 7, 35 °C, and 72 h respectively. PHB production was the best with peptone and glucose as nitrogen and carbon sources at a C/N ratio of (2:1). The strain was able to accumulate 423, 390, 249, 158, and 144 mg/L PHB when pretreated orange, mango, banana, onion peels, and rice straw were used as carbon sources, respectively. The extracted polymer was characterized by Fourier transform infrared (FTIR), nuclear magnetic resonance (NMR), and GC-MS spectroscopy, which confirmed the structure of the polymer as PHB. The isolate B. wiedmannii AS-02 OK576278 can be considered an excellent candidate for industrial production of PHB from agricultural wastes.

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Section: Production Of Phb From Waste Materialsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Bioplastic Production by Bacillus wiedmannii AS-02 OK576278 Using Different Agricultural Wastes

et al. 2021

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“…Rare actinomycetes Aquabacterium sp., A7-Y produced 10.2 g/L of PHB in 5 L fed-batch fermenter after optimization [45]. Streptomyces incanus BK 128 which was isolated from the Rhizosphere of eggplant by Rezk et al [46] used for production of PHB biopolymer using different agricultural waste synthesized maximum quantity of 2.82 g L -1 .…”

Section: Interpretation Of Phb Production By Different Microorganismsmentioning

confidence: 99%

Microbial Production of Biopolymer Polyhydroxybutyrate (PHB): Current Challenges and its Application

Preethi Rathna,

KULANDHAIVEL

2023

ajc

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Plastics are suitable for many industrial applications as they are inexpensive, sturdy, resistant to deterioration and highly versatile. Asadvantageous as plastics made from petroleum are, it remains the most major topic of concern among environmentalists and the scientists. Polyhydroxyalkanoates are biodegradable polyesters produced by a wide range of microbes as a source of energy for metabolism, carbon, and oxidoreduction; since they exhibit some properties, which are remarkably similar to those of polyolefins derived from fossil fuels. he literature review conducted over the past decade reveals that polyhydroxybutyrate (PHB) has the potential physical qualities for food packaging material, single-use plastic goods and medicinal sectors. Polyhydroxybutyrate (PHB) is a bacterial biopolymer that has begun to replace petrochemical plastics due to the present trend towards the efficient utilisation of relatively cheaper substrates for its production. This review discusses explicitly the developments in the analysis of microbial biopolymer PHB and additionally provides an outline of current industrial applications, Production, Extraction methods, genes involved, microbes that are capable of producing PHB, challenges faced during production and degradation of PHB.

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“…The production of PHB is expected to be a good replacement for petroleum-based plastics in a number of industrial applications. A study reported by Rezk et al [ 31 ] showed that wheat bran could be used as a substitute for starch nitrate medium for the production of PHB by Streptomyces incanus BK128. Danial et al [ 32 ] isolated the B. wiedmannii AS-02 OK576278 strain, which was capable of producing PHB when pre-treated with various agricultural residues such as onion peels, banana peels, mango peels, orange peels, mango and rice straw were used as the carbon sources, respectively.…”

Section: Introductionmentioning

confidence: 99%

Characterization and Process Optimization for Enhanced Production of Polyhydroxybutyrate (PHB)-Based Biodegradable Polymer from Bacillus flexus Isolated from Municipal Solid Waste Landfill Site

et al. 2023

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In recent years, there has been a growing interest in bio-based degradable plastics as an alternative to synthetic plastic. Polyhyroxybutyrate (PHB) is a macromolecule produced by bacteria as a part of their metabolism. Bacteria accumulate them as reserve materials when growing under different stress conditions. PHBs can be selected as alternatives for the production of biodegradable plastics because of their fast degradation properties when exposed to natural environmental conditions. Hence, the present study was undertaken in order to isolate the potential PHB-producing bacteria isolated from the municipal solid waste landfill site soil samples collected from the Ha’il region of Saudi Arabia to assess the production of PHB using agro-residues as a carbon source and to evaluate the growth of PHB production. In order to screen the isolates for producing PHB, a dye-based procedure was initially employed. Based on the 16S rRNA analysis of the isolates, Bacillus flexus (B. flexus) accumulated the highest amount of PHB of all the isolates. By using a UV–Vis spectrophotometer and Fourier-transform infrared spectrophotometer (FT-IR), in which a sharp absorption band at 1721.93 cm−1 (C=O stretching of ester), 1273.23 cm−1 (–CH group), multiple bands between 1000 and 1300 cm−1 (stretching of the C–O bond), 2939.53 cm−1 (–CH3 stretching), 2880.39 cm−1 (–CH2 stretching) and 3510.02 cm−1 (terminal –OH group), the extracted polymer was characterized and confirmed its structure as PHB. The highest PHB production by B. flexus was obtained after 48 h of incubation (3.9 g/L) at pH 7.0 (3.7 g/L), 35 °C (3.5 g/L) with glucose (4.1 g/L) and peptone (3.4 g/L) as carbon and nitrogen sources, respectively. As a result of the use of various cheap agricultural wastes, such as rice bran, barley bran, wheat bran, orange peel and banana peel as carbon sources, the strain was found to be capable of accumulating PHB. Using response surface methodology (RSM) for optimization of PHB synthesis using a Box–Behnken design (BBD) proved to be highly effective in increasing the polymer yield of the synthesis. With the optimum conditions obtained from RSM, PHB content can be increased by approximately 1.3-fold when compared to an unoptimized medium, resulting in a significant reduction in production costs. Thus, isolate B. flexus is a highly promising candidate for the production of industrial-size quantities of PHB from agricultural wastes and is capable of removing the environmental concerns associated with synthetic plastics from the industrial production process. Moreover, the successful production of bioplastics using a microbial culture provides a promising avenue for the large-scale production of biodegradable and renewable plastics with potential applications in various industries, including packaging, agriculture and medicine.

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Production of Polyhydroxybutyrate (PHB) from Streptomyces Incanus and the Effect of Gamma Irradiation on its Production

Cited by 3 publications

References 11 publications

Bioplastic Production by Bacillus wiedmannii AS-02 OK576278 Using Different Agricultural Wastes

Bioplastic Production by Bacillus wiedmannii AS-02 OK576278 Using Different Agricultural Wastes

Microbial Production of Biopolymer Polyhydroxybutyrate (PHB): Current Challenges and its Application

Characterization and Process Optimization for Enhanced Production of Polyhydroxybutyrate (PHB)-Based Biodegradable Polymer from Bacillus flexus Isolated from Municipal Solid Waste Landfill Site

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