Production of polyhydroxyalkanoates from inexpensive extruded rice bran and starch by Haloferax mediterranei

Huang, Ting-Yen; Duan, Kow‐Jen; Huang, Shih Yi; Chen, C. Will

doi:10.1007/s10295-006-0098-z

Cited by 177 publications

(100 citation statements)

References 16 publications

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“…Haloferax mediterranei an haloarchaea, having phaEC genes for encoding PHA synthase, although not widely studied, has been reported to produce P(3HB:3HV) utilizing starch, glucose, or other cheaper C sources such as industrial by-products [36][37][38]. Since H. mediterranei survives in extreme salinity, it circumvents the contamination problem and consequently greatly reduces the sterility requirement.…”

Section: Bacterial Pha Synthasesmentioning

confidence: 99%

Challenges and Opportunities for Customizing Polyhydroxyalkanoates

et al. 2015

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Polyhydroxyalkanoates (PHAs) as an alternative to synthetic plastics have been gaining increasing attention. Being natural in their origin, PHAs are completely biodegradable and eco-friendly. However, consistent efforts to exploit this biopolymer over the last few decades have not been able to pull PHAs out of their nascent stage, inspite of being the favorite of the commercial world. The major limitations are: (1) the high production cost, which is due to the high cost of the feed and (2) poor thermal and mechanical properties of polyhydroxybutyrate (PHB), the most commonly produced PHAs. PHAs have the physicochemical properties which are quite comparable to petroleum based plastics, but PHB being homopolymers are quite brittle, less elastic and have thermal properties which are not suitable for processing them into sturdy products. These properties, including melting point (T m ), glass transition temperature (T g ), elastic modulus, tensile strength, elongation etc. can be improved by varying the monomeric composition and molecular weight. These enhanced characteristics can be achieved by modifications in the types of substrates, feeding strategies, culture conditions and/or genetic manipulations.

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Section: Bacterial Pha Synthasesmentioning

confidence: 99%

Challenges and Opportunities for Customizing Polyhydroxyalkanoates

et al. 2015

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“…Similarly while replacing the glucose with corn starches, the volumetric productivity was decreased to 0.29 g −1 L −1 h −1 [73]. However, the productivity is increased to considerable level when a mixture of extruded rice bran and corn starch (1 : 8 w/w), say 0.71 g −1 L −1 h −1 , was obtained [74].…”

Section: Pha Production In Halophilic Archaeamentioning

confidence: 99%

Exploring biodegradable polymer production from marine microbes

Rasu¹,

Arun²

2017

Biodegradable Polymers: Recent Developments and New Perspectives

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“…Los costos de producción dependen del medio de cultivo, proceso fermentativo y extracción del PHA, habiéndose determinado que el costo del sustrato constituye cerca del 50 % del total de producción de PHA . El problema económico puede ser disminuido mediante el mejoramiento del proceso fermentativo y separación del producto (Bello et al, 2008;Mora, 2010), búsqueda de microorganismos más eficientes (Franco et al, 2009;Baca et al, 2010;Razzaq et al, 2010) y el uso de fuentes de carbono más baratas como el glicerol (Beckers et al, 2016) y residuos agrícolas como las cáscaras de Solanum tuberosum L. "papa" (Ramírez et al, 2004;Huang et al, 2006;Rivera y Nevárez, 2009;Cardona et al, 2013), entre otros. Investigaciones realizadas (Díaz, 2007;Oliart et al, 2016;Quillahuaman et al, 2008) demostraron que las bacterias halófilas son heterótrofos capaces de utilizar diversos carbohidratos como fuente de carbono y acumular PHA.…”

Section: Introductionunclassified

Production of polyhydroxyalkanoates by native halophilic bacteria using Solanum tuberosum L. shell starch

Guzmán¹,

Hurtado²,

Carreño³

et al. 2017

Sci. Agropecu

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ResumenEl objetivo del presente estudio fue determinar la concentración de almidón de cáscaras de Solanum tuberosum L. "papa" para la producción de polihidroxialcanoatos, PHA, por bacterias halófilas nativas, como una alternativa para disminuir los costos de producción de estos biopolímeros, posibles reemplazantes de los plásticos provenientes del petróleo. Las bacterias fueron aisladas de muestras de agua de ocho salinas y fueron enriquecidas en caldo HM1 a 30 °C, con 5, 10, 15, 20, 25 y 30 g 100 mL -1 de NaCl. A continuación, se realizaron diluciones, se tomaron alícuotas y se sembraron en agar HM1, obteniéndose 203 aislados de bacterias halófilas que fueron cultivados en caldo HM2 con 10 g.L -1 de glucosa como fuente de carbono y entre las que el 38,92% presentó gránulos de PHA teñidos por Sudan Negro B. Los 20 aislados con gránulos de PHA en el 65 -95 % de las células nuevamente fueron llevadas a fermentación, alcanzando 0,174 -0,889 g.g AbstractThe aim of this study was to determine the concentration of starch Solanum tuberosum L. "potato" peels for the production of polyhydroxyalkanoates, PHA, from native halophilic bacteria as an alternative to reduce production costs of these biopolymers, possible replacements for petrochemical plastics. The bacteria were isolated of water samples of eight saline Lambayeque region and were enriched in HM1 broth at 30 °C with 5, 10, 15, 20, 25 and 30 g 100 mL -1 NaCl. Dilutions were performed subsequently, aliquots were taken and plated on HM1 agar, 203 isolates of halophilic bacteria were obtained, they were grown in HM2 broth with 10 g.L -1 glucose as carbon source and the 38.92% of the isolates showed PHA granules stained by Sudan Black B. Twenty bacteria with PHA granules in 65 -75% over carried to fermentation, reaching between 0.174 to 0.889 g.g -1 of yield Y (p / x). Three isolates were selected with the highest values among which Halomonas sp M4C1 grew and synthesized PHA in HM2 broth with 5, 10, 15 and 20 g.L -1 of starch as carbon source, reaching 0.019; 0.016; 0.007 y 0.006 g.L -1 of PHA, with 0.177; 0.111; 0.056 and 0.066 g.L -1 of biomass after 20, 40, 24 and 16 hours respectively. The highest yield of 0.144 g.g -1 corresponded to 10 g.L -1 of starch demonstrating that this concentration is feasible PHA production by native halophilic bacteria.

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Production of polyhydroxyalkanoates from inexpensive extruded rice bran and starch by Haloferax mediterranei

Cited by 177 publications

References 16 publications

Challenges and Opportunities for Customizing Polyhydroxyalkanoates

Challenges and Opportunities for Customizing Polyhydroxyalkanoates

Exploring biodegradable polymer production from marine microbes

Production of polyhydroxyalkanoates by native halophilic bacteria using Solanum tuberosum L. shell starch

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