ResumenEl objetivo del presente estudio fue determinar la concentración de almidón de cáscaras de Solanum tuberosum L. "papa" para la producción de polihidroxialcanoatos, PHA, por bacterias halófilas nativas, como una alternativa para disminuir los costos de producción de estos biopolímeros, posibles reemplazantes de los plásticos provenientes del petróleo. Las bacterias fueron aisladas de muestras de agua de ocho salinas y fueron enriquecidas en caldo HM1 a 30 °C, con 5, 10, 15, 20, 25 y 30 g 100 mL -1 de NaCl. A continuación, se realizaron diluciones, se tomaron alícuotas y se sembraron en agar HM1, obteniéndose 203 aislados de bacterias halófilas que fueron cultivados en caldo HM2 con 10 g.L -1 de glucosa como fuente de carbono y entre las que el 38,92% presentó gránulos de PHA teñidos por Sudan Negro B. Los 20 aislados con gránulos de PHA en el 65 -95 % de las células nuevamente fueron llevadas a fermentación, alcanzando 0,174 -0,889 g.g AbstractThe aim of this study was to determine the concentration of starch Solanum tuberosum L. "potato" peels for the production of polyhydroxyalkanoates, PHA, from native halophilic bacteria as an alternative to reduce production costs of these biopolymers, possible replacements for petrochemical plastics. The bacteria were isolated of water samples of eight saline Lambayeque region and were enriched in HM1 broth at 30 °C with 5, 10, 15, 20, 25 and 30 g 100 mL -1 NaCl. Dilutions were performed subsequently, aliquots were taken and plated on HM1 agar, 203 isolates of halophilic bacteria were obtained, they were grown in HM2 broth with 10 g.L -1 glucose as carbon source and the 38.92% of the isolates showed PHA granules stained by Sudan Black B. Twenty bacteria with PHA granules in 65 -75% over carried to fermentation, reaching between 0.174 to 0.889 g.g -1 of yield Y (p / x). Three isolates were selected with the highest values among which Halomonas sp M4C1 grew and synthesized PHA in HM2 broth with 5, 10, 15 and 20 g.L -1 of starch as carbon source, reaching 0.019; 0.016; 0.007 y 0.006 g.L -1 of PHA, with 0.177; 0.111; 0.056 and 0.066 g.L -1 of biomass after 20, 40, 24 and 16 hours respectively. The highest yield of 0.144 g.g -1 corresponded to 10 g.L -1 of starch demonstrating that this concentration is feasible PHA production by native halophilic bacteria.
Biodegradable polymers have similar physicochemical properties than conventional polymers, but they can be degradable under controlled conditions in less than 180 days. Thermoplastic starch films have a relative importance because of their low cost, high production and good processability by conventional techniques like melt blending, compression molding, extrusion, among others. However, they present several drawbacks (poor mechanical properties, high water vapor permeability, and retrogradation) that limit their industrial applications. In the present paper, we developed a computer tool, which employs artificial
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