Production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) using agro-industrial effluents with tunable proportion of 3-hydroxyvalerate monomer units

Lemechko, Pierre; Fellic, Magali Le; Bruzaud, Stéphane

doi:10.1016/j.ijbiomac.2019.01.170

Cited by 42 publications

(27 citation statements)

References 29 publications

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“…This ability to exploit various carbon substrates, in addition to its capacity to grow in front of atypical/stressful conditions, make Halomonas sp. SF2003 a versatile strain with a high potential for biotechnological application/use [13,16]. The results of this study identify several potential carbon substrates allowing PHA production and open the door for future tests studying the exploitation of each one.…”

Section: Carbohydrates Bacterial Strains/speciesmentioning

confidence: 85%

“…Eight pure carbohydrates, including five monosaccharides (glucose, fructose, galactose, rhamnose, mannose) and three disaccharides (maltose, melibiose, sucrose) found in food or natural (co-) products, including fruits, vegetables, milk or red algae, have been tested based on data available in the literature and results of previous studies on Halomonas sp. SF2003 [12,13,16,31,48]. The results obtained with Halomonas sp.…”

Section: Discussionmentioning

confidence: 97%

“…Then, the solvent was evaporated, and PHA films were solvent-casted in a glass Petri dish, at room temperature, until a constant weight obtained. The PHA content was determined as the PHA to cell dry weight (CDW) percent ratio [12,16].…”

Section: Pha Extractionmentioning

confidence: 99%

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PHA Production and PHA Synthases of the Halophilic Bacterium Halomonas sp. SF2003

et al. 2020

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Among the different tools which can be studied and managed to tailor-make polyhydroxyalkanoates (PHAs) and enhance their production, bacterial strain and carbon substrates are essential. The assimilation of carbon sources is dependent on bacterial strain’s metabolism and consequently cannot be dissociated. Both must wisely be studied and well selected to ensure the highest production yield of PHAs. Halomonas sp. SF2003 is a marine bacterium already identified as a PHA-producing strain and especially of poly-3-hydroxybutyrate (P-3HB) and poly-3-hydroxybutyrate-co-3-hydroxyvalerate (P-3HB-co-3HV). Previous studies have identified different genes potentially involved in PHA production by Halomonas sp. SF2003, including two phaC genes with atypical characteristics, phaC1 and phaC2. At the same time, an interesting adaptability of the strain in front of various growth conditions was highlighted, making it a good candidate for biotechnological applications. To continue the characterization of Halomonas sp. SF2003, the screening of carbon substrates exploitable for PHA production was performed as well as production tests. Additionally, the functionality of both PHA synthases PhaC1 and PhaC2 was investigated, with an in silico study and the production of transformant strains, in order to confirm and to understand the role of each one on PHA production. The results of this study confirm the adaptability of the strain and its ability to exploit various carbon substrates, in pure or mixed form, for PHA production. Individual expression of PhaC1 and PhaC2 synthases in a non-PHA-producing strain, Cupriavidus necator H16 PHB¯4 (DSM 541), allows obtaining PHA production, demonstrating at the same time, functionality and differences between both PHA synthases. All the results of this study confirm the biotechnological interest in Halomonas sp. SF2003.

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Section: Carbohydrates Bacterial Strains/speciesmentioning

confidence: 85%

Section: Discussionmentioning

confidence: 97%

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PHA Production and PHA Synthases of the Halophilic Bacterium Halomonas sp. SF2003

et al. 2020

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“…PHAs are synthesized in the mitochondria of a wide range of Gram-negative (G−) and Gram-positive (G+) microorganisms [3]. They can be divided into short chain length (scl-PHAs) with monomers with three to five carbon atoms and medium chain length (mcl-PHAs) with monomers with more than six carbons [4]. Among them, scl-PHAs are the most…”

Section: Introductionmentioning

confidence: 99%

“…Nanomaterials 2020, 10, 506 2 of 26 studied, including the poly(3-hydroxybutyrate) (PHB or P3HB) homopolyester and its copolymer poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV). The latter shows lower crystallinity and melting point as well as greater flexibility, which broadens its industrial applicability [4,5]. In order to confer active properties to PHAs, several substances can be incorporated into the biopolymers such as essential oils (EOs) and inorganic or metal nanoparticles (MNPs) [6,7].EOs are the product of the secondary metabolism of plants, separated from the aqueous phase, which is formed by various volatile components such as terpenes, alcohols, acids, esters, epoxies, aldehydes, ketones, amines, and sulphides, among others [8].…”

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confidence: 99%

Electrospun Active Biopapers of Food Waste Derived Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) with Short-Term and Long-Term Antimicrobial Performance

et al. 2020

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This research reports about the development by electrospinning of fiber-based films made of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) derived from fermented fruit waste, so-called bio-papers, with enhanced antimicrobial performance. To this end, different combinations of oregano essential oil (OEO) and zinc oxide nanoparticles (ZnONPs) were added to PHBV solutions and electrospun into mats that were, thereafter, converted into homogeneous and continuous films of 130 µm. The morphology, optical, thermal, mechanical properties, crystallinity, and migration into food simulants of the resultant PHBV-based bio-papers were evaluated and their antimicrobial properties were assessed against Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) in both open and closed systems. It was observed that the antimicrobial activity decreased after 15 days due to the release of the volatile compounds, whereas the bio-papers filled with ZnONPs showed high antimicrobial activity for up to 48 days. The electrospun PHBV biopapers containing 2.5 wt% OEO + 2.25 wt% ZnONPs successfully provided the most optimal activity for short and long periods against both bacteria. Nanomaterials 2020, 10, 506 2 of 26 studied, including the poly(3-hydroxybutyrate) (PHB or P3HB) homopolyester and its copolymer poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV). The latter shows lower crystallinity and melting point as well as greater flexibility, which broadens its industrial applicability [4,5]. In order to confer active properties to PHAs, several substances can be incorporated into the biopolymers such as essential oils (EOs) and inorganic or metal nanoparticles (MNPs) [6,7].EOs are the product of the secondary metabolism of plants, separated from the aqueous phase, which is formed by various volatile components such as terpenes, alcohols, acids, esters, epoxies, aldehydes, ketones, amines, and sulphides, among others [8]. This wide range of compounds are responsible for the strong biological activity and also the antioxidant, antimicrobial, antifungal, and antiviral properties of plants [9]. In addition, EOs are biologically safe and have a low risk of causing resistance in pathogens [10]. In addition, they are classified as Generally Recognized as Safe (GRAS) by the U.S. Food and Drug Administration (FDA) [11]. Among them, oregano essential oil (OEO) is one of the most important and it is widely used in the pharmaceutical, food, and cosmetics industries [12]. It comes from the genus Origanum that belongs to the Lamiaceae family [13], which is constituted by more than 38 monoterpenoids [14]. Their main active compounds are carvacrol, thymol, p-cymene, and γ-terpinene, which are responsible for its high antimicrobial and antioxidant activities [15,16]. These active compounds have been particularly related to the inhibition of G− bacteria such as Escherichia coli (E. coli), Pseudomonas aeruginosa (P. aeruginosa), and Salmonella typhimurium (S. typhimurium) and G+ bacteria including Staphylococcus aureus (S. aureus), Listeria mo...

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Ester and Amide Polymers

2019

The Chemistry of Bio‐based Polymers

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Production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) using agro-industrial effluents with tunable proportion of 3-hydroxyvalerate monomer units

Cited by 42 publications

References 29 publications

PHA Production and PHA Synthases of the Halophilic Bacterium Halomonas sp. SF2003

PHA Production and PHA Synthases of the Halophilic Bacterium Halomonas sp. SF2003

Electrospun Active Biopapers of Food Waste Derived Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) with Short-Term and Long-Term Antimicrobial Performance

Ester and Amide Polymers

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