Production of Plantlets from the Female Gametophytes of Ephedra foliata Boiss

Konar, R. N.; Singh, Maninder

doi:10.1016/s0044-328x(79)80031-8

Cited by 19 publications

(8 citation statements)

References 6 publications

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“…14). In order to allow shoots to grow out, it is necessary to transfer them to a medium with low or zero growth regulators, confirming earlier observations (Konar and Singh 1979;Singh et al 1981). …”

Section: Organogenesissupporting

confidence: 81%

“…Regeneration of shoots has been reported from haploid female gametophyte-derived explants of E. foliata (Konar and Singh 1979;Singh et al 1981;Bhatnagar and Singh 1984). Shoots and roots grew out from meristemoids following optimisation of growth regulators in the medium.…”

Section: Organogenesismentioning

confidence: 99%

“…high alkaloidyielding) and to speed up the production of select Ephedra plants from conventional hybridisation or mutagenesis. Prior to our own studies (O'Dowd and Richardson 1993a), only two Ephedra species, E. foliata and E. gerardiana, had been micropropagated in vitro and rooting had been problematic (Sankhla et al 1967b;Ramawat and Arya 1976;Konar and Singh 1979;Bhatnagar and Singh 1984;Arya and Ramawat 1988).…”

Section: Bapmentioning

confidence: 99%

See 2 more Smart Citations

X Ephedra Species: In Vitro Culture, Micropropagation, and the Production of Ephedrine and Other Alkaloids

O'Dowd

McCauley

Wilson

et al. 1998

Biotechnology in Agriculture and Forestry

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Section: Organogenesissupporting

confidence: 81%

Section: Organogenesismentioning

confidence: 99%

Section: Bapmentioning

confidence: 99%

See 1 more Smart Citation

X Ephedra Species: In Vitro Culture, Micropropagation, and the Production of Ephedrine and Other Alkaloids

O'Dowd

McCauley

Wilson

et al. 1998

Biotechnology in Agriculture and Forestry

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“…It is used in traditional medicine to treat allergies, bronchial asthma, chills, colds, coughs, edema, fever, flu, headaches. This plant also shows antimicrobial and anticancer activities (Konar & Singh (1979); Nawwar et al (1985); O'Dowd et al (1998)). It has been a natural source of alkaloids such as ephedrine, pseudoephedrine, and other related compounds (Parsaeimehr et al (2010).…”

Section: Introductionmentioning

confidence: 99%

Analysis of Phenolic and Flavonoids of Wild Ephedra Alata Plant Extracts by Lc/Pda and Lc/MS and Their Antioxidant Activity

Al‐Rimawi¹,

Abu‐Lafi²,

Abbadi³

et al. 2017

Afr J Tradit Complement Altern Med

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Background: Ephedra is among Palestinian medicinal plants that are traditionally used in folkloric medicine for treating many diseases. Ephedra is known to have antibacterial and antioxidant effects. The goal of this study is to evaluate the antioxidant activity of different extracts from the Ephedra alata plant growing wild in Palestine, and to analyze their phenolic and flavonoid constituents by HPLC/PDA and HPLC/MS. Materials and Methods: Samples of the Ephedra alata plant grown wild in Palestine were extracted with three different solvents namely, 100% water, 80% ethanol, and 100% ethanol. The extracts were analyzed for their total phenolic content (TPC), total flavonoid content (TFC), antioxidant activity (AA), as well as phenolic and flavonoids content by HPLC/PDA/MS. Results:The results revealed that the polarity of the extraction solvent affects the TPC, TFC, and AA of extracts. It was found that both TPC and AA are highest for plant extracted with 80% ethanol, followed by 100% ethanol, and finally with 100% water. TFC however was highest in the following order: 100% ethanol > 80% ethanol > water. Pearson correlation indicated that there is a significant correlation between AA and TPC, but there is no correlation between AA and TFC. Simultaneous HPLC-PDA and UHPLC-MS analysis of the ethanolic plant extracts revealed the presence of Luteolin-7-O-glucuronide flavone, Myricetin 3-rhamnoside and some other major polyphenolic compounds that share myricetin skeleton. Conclusion Ephedra alata extract is rich in potent falvonoid glycosidic compounds as revealed by their similar overlaid UV-Vis spectra and UHPLC-MS results. On the basis of these findings, it is concluded that Ephedra alata constitutes a natural source of potent antioxidants that may prevent many diseases and could be potentially used in food, cosmetics, and pharmaceutical products.

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“…15,16 In our area, this indigenous plant that is called "Hamisheh Zaroor" means "Always necessary" has been used for many years in the treatment of allergies, bronchial asthma, chills, coughs, and has been a source of natural product for alkaloids and other related compounds. [17][18][19] There are several reports concerning in vitro antibacterial effect against different bacterial species, including Staphylococcus aureus, Bacillus antheracis, B. diphtheria, B. dysenteriae, B. typhosus and Pseudomonas aeruginosa.…”

Section: Introductionmentioning

confidence: 99%

In Vitro Assessment of the Protoscolicidal Activities of the Ephedra major Methanol Extracts

Zibaei¹,

Sepideh²,

Zohreh³

et al. 2017

Int J Enteric Pathog

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Background: The basic approaches for the treatment of human hydatidosis are surgery and chemotherapy. The risk of spillage of protoscoleces cannot be underestimated during surgery. Objectives: This study aimed to evaluate the scolicidal activity of Ephedra major methanol extracts against protoscoleces of hydatid cysts. Materials and Methods: Various concentrations of the methanolic extracts (0.01%-0.001%, mg/mL) of different parts of E. major were used for exposure times of 10, 20, 30, and 60 minutes. Results: Different extracts of E. major were tested, 0.1% concentration had strong scolicidal activity in 60 minutes. The stem extracts of 0.1% had very strong scolicidal effects in 60 minutes of exposure time and the mortality rate decreased with the lower concentration. Conclusion: Findings showed that scolicidal effects of E. major root extracts against cystic echinococcosis protoscoleces were less effective, while the stem and leaf extracts demonstrated more activities, respectively.

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Production of Plantlets from the Female Gametophytes of Ephedra foliata Boiss

Cited by 19 publications

References 6 publications

X Ephedra Species: In Vitro Culture, Micropropagation, and the Production of Ephedrine and Other Alkaloids

X Ephedra Species: In Vitro Culture, Micropropagation, and the Production of Ephedrine and Other Alkaloids

Analysis of Phenolic and Flavonoids of Wild Ephedra Alata Plant Extracts by Lc/Pda and Lc/MS and Their Antioxidant Activity

In Vitro Assessment of the Protoscolicidal Activities of the Ephedra major Methanol Extracts

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