Production of Nucleic Acid-Related Substances by Fermentative Processes. V. Accumulation of Inosinic Acid by an Adenine-Auxotroph of Micrococcus Glutamicus

Nakayama, Kiyoshi; Suzuki, Takeo; Sato, Zenroku; Kinoshita, Shukuo

doi:10.2323/jgam.10.133

Cited by 19 publications

(5 citation statements)

References 5 publications

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“…Certain of these mutants excrete IMP. A similar finding was recently published by Nakayama et al (1964). Further mutation to xanthine dependence leads to increases in excretion of IMP.…”

supporting

confidence: 88%

Production of Xanthosine-5′-Monophosphate and Inosine-5′-Monophosphate by Auxotrophic Mutants of a Coryneform Bacterium

Demain

Jackson

Vitali

et al. 1965

Applied Microbiology

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Production of xanthosine-5'-monophosphate and inosine-5'-monophosphate by auxotrophic mutants of a coryneform bacterium. Appl. Microbiol. 13:757-761. 1965-Although most microorganisms with genetic blocks in the purine nucleotide sequence excrete breakdown products, a coryneform bacterium was found to accumulate intact 5'-nucleotides in the extracellular medium. Adenineless mutants accumulated 0.4 to 0.6 g of inosine-5'-monophosphate per liter of broth. The yield of this nucleotide was increased to 0.8 to 0.9 g per liter when such mutants were mutated to xanthine dependence. Induction of a specific guanine requirement in adenineless auxotrophs resulted in cultures capable of producing high yields of xanthosine-5'-monophosphate (3 to 4 g per liter). Pure xanthosine-5'-monophosphate was isolated from broth by a procedure involving ion-exchange chromatography, charcoal adsorption, and barium precipitation.

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“…Certain of these mutants excrete IMP. A similar finding was recently published by Nakayama et al (1964). Further mutation to xanthine dependence leads to increases in excretion of IMP.…”

supporting

confidence: 88%

Production of Xanthosine-5′-Monophosphate and Inosine-5′-Monophosphate by Auxotrophic Mutants of a Coryneform Bacterium

Demain

Jackson

Vitali

et al. 1965

Applied Microbiology

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“…The procedures for direct production of IMP from carbon and nitrogen sources have been reported in a few papers (5,8) but high yields of the acid have not been attained. We have been conducting induction of biochemical mutants from B. ammoniagenes using various mutagens and have obtained a mutant, induced by ultraviolet light irradiation, which accumulates large amounts of IMP.…”

mentioning

confidence: 99%

Production of Nucleic Acid-related Substances by Fermentative Processes

Furuya

Abe

Kinoshita

1968

Applied Microbiology

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The accumulation of 5'-inosinic acid (IMP) by a mutant, KY 13102, induced from Brevibacterium ammoniagenes ATCC 6872 by ultraviolet light irradiation, was examined. Although growth was stimulated by adenine or adenosine, the microorganism showed fair growth in the medium containing amino acids but no adenine. Among six kinds of natural nutrients tested, meat extract and Casamino Acids were suitable for the accumulation of IMP. Manganese ion strongly affected growth, the accumulation of IMP and hypoxanthine, and cell morphology. Among amino acids tested, L-methionine, L-proline, and L-valine stimulated IMP accumulation. In the medium containing 1.0 g of L-proline per liter, 12.8 mg of IMP per ml was accumulated. The mechanism of IMP accumulation by the mutant is discussed.

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“…Already in the 1960s, it was found that an adenine-auxotrophic Micrococcus glutamicus strain accumulated inosinic acid to a concentration of 0.75 g/L (IMP Na 2 x 7.5 H 2 O) (Nakayama et al, 1964 ). Production was also achieved in Corynebacterium ammoniagenes strains obtained after random mutagenesis experiments, which were capable of accumulating 7.5 g/L inosinic acid (Tomita et al, 1991 ).…”

Section: Metabolic Engineering For the Microbial Production Of Food Amentioning

confidence: 99%

Engineered Microorganisms for the Production of Food Additives Approved by the European Union—A Systematic Analysis

Kallscheuer

2018

Front. Microbiol.

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In the 1950s, the idea of a single harmonized list of food additives for the European Union arose. Already in 1962, the E-classification system, a robust food safety system intended to protect consumers from possible food-related risks, was introduced. Initially, it was restricted to colorants, but at later stages also preservatives, antioxidants, emulsifiers, stabilizers, thickeners, gelling agents, sweeteners, and flavorings were included. Currently, the list of substances authorized by the European Food Safety Authority (EFSA) (referred to as “E numbers”) comprises 316 natural or artificial substances including small organic molecules, metals, salts, but also more complex compounds such as plant extracts and polymers. Low overall concentrations of such compounds in natural producers due to inherent regulation mechanisms or production processes based on non-regenerative carbon sources led to an increasing interest in establishing more reliable and sustainable production platforms. In this context, microorganisms have received significant attention as alternative sources providing access to these compounds. Scientific advancements in the fields of molecular biology and genetic engineering opened the door toward using engineered microorganisms for overproduction of metabolites of their carbon metabolism such as carboxylic acids and amino acids. In addition, entire pathways, e.g., of plant origin, were functionally introduced into microorganisms, which holds the promise to get access to an even broader range of accessible products. The aim of this review article is to give a systematic overview on current efforts during construction and application of microbial cell factories for the production of food additives listed in the EU “E numbers” catalog. The review is focused on metabolic engineering strategies of industrially relevant production hosts also discussing current bottlenecks in the underlying metabolic pathways and how they can be addressed in the future.

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Production of Nucleic Acid-Related Substances by Fermentative Processes. V. Accumulation of Inosinic Acid by an Adenine-Auxotroph of Micrococcus Glutamicus

Cited by 19 publications

References 5 publications

Production of Xanthosine-5′-Monophosphate and Inosine-5′-Monophosphate by Auxotrophic Mutants of a Coryneform Bacterium

Production of Xanthosine-5′-Monophosphate and Inosine-5′-Monophosphate by Auxotrophic Mutants of a Coryneform Bacterium

Production of Nucleic Acid-related Substances by Fermentative Processes

Engineered Microorganisms for the Production of Food Additives Approved by the European Union—A Systematic Analysis

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