Production of Newcastle Disease Virus by Vero Cells Grown on Cytodex 1 Microcarriers in a 2-Litre Stirred Tank Bioreactor

Arifin, Mohd Azmir; Mel, Maizirwan; Karim, Mohamed Ismail Abdul; Ideris, Aini

doi:10.1155/2010/586363

Cited by 29 publications

(27 citation statements)

References 13 publications

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“…Survival rate (%) = [(As -Ab)/(Ac -Ab)] × 100% [4] where As represents the test wells (containing cell medium, WST-8, and chitosan nanoparticles); Ac represents the control wells (containing cell medium and WST-8); and Ab represents the blank wells (containing cell medium only).…”

Section: Evaluation Of the Safety Of Pfndv-cs-nps In Vitro Cytotoxicimentioning

confidence: 99%

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Preparation and efficacy of Newcastle disease virus DNA vaccine encapsulated in chitosan nanoparticles

Zhao¹,

Zhang²,

Zhang³

et al. 2014

IJN

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Optimal preparation conditions of Newcastle disease virus (NDV) F gene deoxyribonucleic acid (DNA) vaccine encapsulated in chitosan nanoparticles (pFNDV-CS-NPs) were determined. The pFNDV-CS-NPs were prepared according to a complex coacervation method. The pFNDV-CS-NPs were produced with good morphology, high stability, a mean diameter of 199.5 nm, encapsulation efficiency of 98.37%±0.87%, loading capacity of 36.12%±0.19%, and a zeta potential of +12.11 mV. The in vitro release assay showed that the plasmid DNA was sustainably released from the pFNDV-CS-NPs, up to 82.9%±2.9% of the total amount. Cell transfection test indicated that the vaccine expressed the F gene in cells and maintained good bioactivity. Additionally, the safety of mucosal immunity delivery system of the pFNDV-CS-NPs was also tested in vitro by cell cytotoxicity and in vivo by safety test in chickens. In vivo immunization showed that better immune responses of specific pathogen-free chickens immunized with the pFNDV-CS-NPs were induced, and prolonged release of the plasmid DNA was achieved compared to the chickens immunized with the control plasmid. This study lays the foundation for the further development of mucosal vaccines and drugs encapsulated in chitosan nanoparticles.

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Section: Evaluation Of the Safety Of Pfndv-cs-nps In Vitro Cytotoxicimentioning

confidence: 99%

“…4 There are no treatments available for ND. Vaccination, however, is an effective method to control ND.…”

Section: Introductionmentioning

confidence: 99%

Preparation and efficacy of Newcastle disease virus DNA vaccine encapsulated in chitosan nanoparticles

Zhao¹,

Zhang²,

Zhang³

et al. 2014

IJN

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“…8,9 There are no treatments available for ND except vaccination. The NDV live vaccines and inactivated vaccines are used universally to control ND.…”

Section: Newcastle Disease Virus Vaccinementioning

confidence: 99%

Newcastle disease virus vaccine encapsulated in biodegradable nanoparticles for mucosal delivery of a human vaccine

Sun¹,

Zhang

Shi

et al. 2014

Human Vaccines & Immunotherapeutics

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A n overwhelming number of medicines on the market are oral medicine with the disadvantage of lower bioavailability universally. Newcastle disease (ND) has become a serious disease that threatens the poultry industries in many countries, and there are no treatments available for ND. The biodegradable materials could be surface modified and protect antigen or DNA from damage. Furthermore, nanoparticles are also a potential drug delivery with proper size. However, Newcastle disease virus (NDV) vaccines encapsulated in nanoparticles were widely used due to their proved a high safety and induced quicker and better mucosal and humoral immune responses. Here we review the results of mucosal immune delivery system for ND. Due to the safety, low toxicity, and better immunogenicity of the mucosal immune delivery system, our studies provide a clearly view that used the biodegradable materials to research and develop the human vaccines to save more patients' lives. These promising results provide a foundation for testing the approach in humans.

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“…The efficacy of NDV vaccines depends on the induction of F glycoproteins. 2 Traditional NDV vaccines include two types: inactivated vaccines and attenuated live vaccines. 3 Both of these traditional types of vaccines have important limitations, including reversion to virulence and induction of respiratory pathological changes.…”

Section: Introductionmentioning

confidence: 99%

“…In vitro transfection and western blot analysis of the pFDNa-cs/Plga-NPs 293T cells were grown in poly-lysine-treated 6-well plates and cultured at 37°C in a CO 2 incubator. An in vitro transfection experiment was carried out according to the instructions from the Lipofectamine™ 2000 reagent kit (Invitrogen™; Life Technologies Corp, Carlsbad, CA, USA), using four groups: 1) the naked plasmid DNA group; 2) the pFDNA-CS/PLGA-NP transfected group; 3) the blank CS/PLGA-NP group; and 4) the negative cell control group.…”

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confidence: 99%

Chitosan-coated poly(lactic-co-glycolic) acid nanoparticles as an efficient delivery system for Newcastle disease virus DNA vaccine

Zhao¹,

Zhang²,

Zhang³

et al. 2014

IJN

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We determined the efficacy and safety of chitosan (CS)-coated poly(lactic-co-glycolic) acid (PLGA) nanoparticles (NPs) as a delivery system for a vaccine to protect chickens against Newcastle disease virus (NDV). The newly constructed vaccine contained DNA (the F gene) of NDV. The Newcastle disease virus (NDV) F gene deoxyribonucleic acid (DNA) plasmid (pFDNA)-CS/PLGA-NPs were spherical (diameter =699.1±5.21 nm [mean ± standard deviation]) and smooth, with an encapsulation efficiency of 98.1% and a Zeta potential of +6.35 mV. An in vitro release assay indicated that CS controlled the burst release of plasmid DNA, such that up to 67.4% of the entire quantity of plasmid DNA was steadily released from the pFDNA-CS/PLGA-NPs. An in vitro expression assay indicated that the expression of nanoparticles (NPs) was maintained in the NPs. In an immunization test with specific pathogen-free chickens, the pFDNA-CS/PLGA-NPs induced stronger cellular, humoral, and mucosal immune responses than the plasmid DNA vaccine alone. The pFDNA-CS/PLGA-NPs did not harm 293T cells in an in vitro assay and did not harm chickens in an in vivo assay. Overall, the results indicated that CS-coated PLGA NPs can serve as an efficient and safe mucosal immune delivery system for NDV DNA vaccine.

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Production of Newcastle Disease Virus by Vero Cells Grown on Cytodex 1 Microcarriers in a 2-Litre Stirred Tank Bioreactor

Cited by 29 publications

References 13 publications

Preparation and efficacy of Newcastle disease virus DNA vaccine encapsulated in chitosan nanoparticles

Preparation and efficacy of Newcastle disease virus DNA vaccine encapsulated in chitosan nanoparticles

Newcastle disease virus vaccine encapsulated in biodegradable nanoparticles for mucosal delivery of a human vaccine

Chitosan-coated poly(lactic-co-glycolic) acid nanoparticles as an efficient delivery system for Newcastle disease virus DNA vaccine

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