Production of monoclonal antibodies and ELISA for thebaine and codeine

Abstract

The ratio of hapten and bovine serum albumin in antigen conjugate was exactly determined by matrix-assisted laser desorption/ionization mass spectrometry. Monoclonal antibodies against thebaine and codeine were produced by hybridoma fused with the sprenocytes immunized with thebaine- and codeine-bovine serum albumin conjugate and HAT-sensitive mouse myeloma cell line, P3-X63-Ag8-653. No cross-reaction of anti-thebaine antibody against morphine was observed. Very small cross-reaction appeared in codeine (0.004%). The cross-reaction of anti-codeine antibody against morphine and thebaine was 2.97 and 5.98%, respectively. The full measuring range of the assay extends from 60 pg mL to 1 ng mL for thebaine and 1 ng mL to 100 ng mL for codeine.

Development of an Assay System for Saikosaponin a Using Anti-saikosaponin a Monoclonal Antibodies

“…4) Although immunoassay systems using monoclonal antibody (MAb) are now indispensable in various investigations, not as many are available for naturally occurring bioactive compounds having smaller molecular weights, except against drugs like morphine. In our ongoing study of the formation of MAbs against naturally occurring bioactive compounds, we have established MAbs against forskolin, 5) solamargine, 6) opium alkaloid, 7) marijuana compounds, 8) glycyrrhizin, 9) crocin, 10) ginsenoside Rb1 11) and Rg1, 12) and developed their applications, such as a new eastern blotting 13) and an immuno affinity column chromatography. 14) No formation of MAb against SSa has been reported yet, although immunological approaches for assaying quantities of SSa using the anti-SSa polyclonal antibody have been investigated by Jung et al 15) Polyclonal antibodies cross-reacted with random proteins non-specifically and induced a problem for the quantitation of hapten in plasma.…”

Quantitative analysis of ginsenosides Rb1, Rg1, and Re in American ginseng berry and flower samples by ELISA using monoclonal antibodies

“…In our ongoing study of the production of monoclonal antibodies (MAbs) against naturally occurring bioactive compounds, we have produced MAbs against, e.g., forskolin [6,7], opium alkaloids [8], marihuana compounds [9], solamargine [10], and sennoside A [11], and applied these to an enzyme-linked immunosorbent assay (ELISA). We also prepared the MAbs against ginsenosides Rb1, Rg1, and Re (GRb1, GRg1, and GRe) [12][13][14].…”

Production of monoclonal antibodies against antitumor cyclohexapeptide RA-VII from Rubia cordifolia and their characterization

“…One recently developed method to answer the request is an immunoassay system [10][11][12][13][14] in which specific monoclonal antibodies (MAbs) against small molecular weight compounds are prepared and, by using the specific affinity of the MAbs to antigen, reliable quantitative and qualitative analyses of those compounds can be performed.…”