Production of Monoclonal Antibodies against Red Sea Bream Iridovirus.

Nakajima, Kazuhiro; Sorimachi, Minoru

doi:10.3147/jsfp.30.47

Cited by 50 publications

(26 citation statements)

References 18 publications

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“…However, the applicability of these meth ods in virology is hampered by the availability of a sus ceptible cell line to enable the quantification of viruses (Nakajima and Sorimachi, 1994). To date, Giemsa staining and an immunofluorescence test (IF) using a monoclonal antibody (Mab) are the methods commonly used for RSIV diagnosis (Inouye et al, 1992;Nakajima and Sorimachi, 1995).…”

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confidence: 99%

Development of a Real-time PCR Assay for the Detection and Quantification of Red Seabream Iridovirus (RSIV).

Caipang¹,

Hirono²,

Aoki³

2003

Fish Pathol.

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Development of a Real-time PCR Assay for the Detection and Quantification of Red Seabream Iridovirus (RSIV).

Caipang¹,

Hirono²,

Aoki³

2003

Fish Pathol.

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“…and Japanese parrot fish Oplegnathus fasciatus. The caus ative viruses having characteristics of the family Iridoviridae and antigenically related to RSIV were isolated from these fishes (Nakajima and Sorimachi, 1995).…”

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Immunofluorescence Test for the Rapid Diagnosis of Red Sea Bream Iridovirus Infection using Monoclonal Antibody.

et al. 1995

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By using indirect immunofluorescence (IF) test with a monoclonal antibody (MAb) against red sea bream iridovirus (RSIV), the detection method of the RSIV specific antigen was examined from imprints or frozen sections of spleens of artificially infected red sea bream Pagrus major. Immunofluorescencepositive cells were not detected from uninfected fish and those of day 1 and 3 post infection. However, a few IF-positive cells were detected from day 5 post infection and many IF-positive cells including typically enlarged cells were detected from moribund or dead fish. This method was applied to 11 species of cultured marine fishes that showed disease condition or suspected iridovirus infection. The IF-positive cells were detected from all these naturally infected fishes of 11 species. The detection rate of infected fish by IF test using MAb was much higher than that by the currently used Giemsa staining method. The results indicate that the IF test using the RSIV specific MAb is a rapid and effective method for confirmative diagnosis of the iridovirus infection in cultured marine fishes.

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“…It is possible to consider that some peptides such as virus-induced polypeptides in the RSIV-infected cell culture also had potent substances to induce immunity. In fact we have shown that the MAb which was used for the detection of viral antigens in the present experiment recognized 230-180 kDa polypep tide which may possibly be a virus-induced polypeptide rather than the structural protein of the virus (Nakajima & Sorimachi, 1995). What kind of epitope in the vac cine contributes to induce the immunity is now being studied in our laboratory.…”

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confidence: 59%

“…and Japanese parrot fish (Oplegnathus fasciatus). For disease diagnosis, Giemsa staining and immunofluorescence (IF) test using a mono clonal antibody (MAb) have been used (Inouye et al, 1992, Nakajima and Sorimachi, 1995. However, no control measures for the disease have been reported.…”

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confidence: 99%