Production of <i>Bacillus sphaericus</i> Entomopathogenic Biomass Using Brewery Residues

Abstract

The use of brewery residues--yeast and trub--has been evaluated aiming to minimize the costs of the industrial production of Bacillus sphaericus-based bioinsecticide. Both brewery residues promoted growth and sporulation of the three B. sphaericus strains that were isolated from Brazilian soils (S1, S2, and S20). However, distinct growth and sporulation behaviors were observed in relation to the different nutritional conditions and strain used. The maximum sporulation percentage was obtained through the cultivation of S20 strain in brewery residual yeast. In general, the entomopathogenic biomasses produced showed good results for toxicity to Culex larvae. The minimum values of larvae population (LC50) were observed for the S20 strain grown on yeast brewery residue-containing media. After fermentation, a considerable decrease in the organic material of alternative media was verified, although the residual values were still higher than that considered appropriate for effluent discharge.

Residual Brewing Yeast as Substrate for Co-Production of Cell Biomass and Biofilm Using Candida maltosa SM4

“…Brewery residues constitute a major problem of the brewing business due to the large volumes that are generated on a daily basis [16]. Although the use of brewer's spent grain (BSG), the main brewery by-product, in animal feed [17,18] and other areas [19], is a well-investigated issue, a holistic approach to the exploitation of all residues of the brewing industry is still challenging, and production of high added-value products along with complementary techno-economic analysis is still in its early development [20,21].…”