Egg yolk enhances early sporulation and toxicity of Bacillus sphaericus H5a5b for small-scale production of a mosquito control agent

Prabakaran, G.; Hoti, L.

doi:10.1016/j.actatropica.2008.07.007

Acta Tropica

2008

DOI: 10.1016/j.actatropica.2008.07.007

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Egg yolk enhances early sporulation and toxicity of Bacillus sphaericus H5a5b for small-scale production of a mosquito control agent

G. Prabakaran

L. Hoti

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Cited by 3 publications

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Synergistic Activity Between S-Layer Protein and Spore–Crystal Preparations from Lysinibacillus sphaericus Against Culex quinquefasciatus Larvae

Lozano

Dussán

2017

Curr Microbiol

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Lysinibacillus sphaericus is used for the biological control of mosquitoes. The main toxicity mechanism of pathogenic strains is a binary toxin produced during sporulation. S-layer is a proteinaceous structure on the surface of bacteria; its functions have been involved in the interaction between bacterial cells and the environment, for example, as protective coats, surface recognition, and biological control. In L. sphaericus, S-layer protein (SlpC) is expressed in vegetative cells, and is also found in spore-crystal preparations; it has larvicidal activity in Culex spp. In this study, partial and completed sporulated culture toxicities were compared; also, S-layer protein and spore-crystal proteins were tested against Culex quinquefasciatus larvae for possible interactions. Larvicidal activity obtained with a combination of SlpC and spore-crystal proteins from strain III(3)7 showed no significant interaction, whereas, combinations of both preparations from strain 2362 showed synergistic effect. The highest synergistic activity observed was between spore protein complex from strain 2362 and SlpC from III(3)7. S-layer protein could be considered a good alternative in formulation improvement, for biological control of mosquitoes.

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Synergistic Activity Between S-Layer Protein and Spore–Crystal Preparations from Lysinibacillus sphaericus Against Culex quinquefasciatus Larvae

Lozano

Dussán

2017

Curr Microbiol

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Development of Cost Effective Medium for Production of Bacillus sphaericus Strain Isolated from Assam, India

Yadav¹,

Dhiman²,

Baruah³

et al. 2011

Microbiology J.

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Improvement of Sporulation Conditions of a New Strain of <i>Bacillus amyloliquefaciens</i> in Liquid Fermentation

Díaz-García¹,

García-Riaño²,

Zapata-Narvaez³

2015

ABB

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The desirable active ingredients for the development of bioproducts based in Bacillus sp. for the control of soil pathogens are the spores because these structures exhibit more resistance and stability to conditions present during the fermentation, formulation, and storage processes. To improve the sporulation of a native strain of Bacillus amyloliquefaciens (Bs006) using liquid fermentation, some modifications in the concentrations of the components in a previously standardized culture media were made. Subsequently, five sporulation inducers (iron nitrate, mixture of salts, peroxide hydrogen, temperature, and initial cell concentration) were evaluated. The treatment with a mixture of salts in combination with an initial cell concentration of 1 × 10 8 cells/ml was selected because a final spore concentration of 1.05 × 10 10 spores/ml after 66 hours with a fully substrate consumption and sporulation efficiency of 88.7% was obtained. To demonstrate the biological activity of B. amyloliquefaciens Bs006 in Cape gooseberry seedlings, a greenhouse bioassay was conducted, and statistical differences in plant growth-promoting parameters compared with previous media were not found. Additionally, the proposed modified media (coded as JM) presented a benefit-cost ratio 2.65 times higher compared with the baseline media.

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Egg yolk enhances early sporulation and toxicity of Bacillus sphaericus H5a5b for small-scale production of a mosquito control agent

Cited by 3 publications

References 10 publications

Synergistic Activity Between S-Layer Protein and Spore–Crystal Preparations from Lysinibacillus sphaericus Against Culex quinquefasciatus Larvae

Synergistic Activity Between S-Layer Protein and Spore–Crystal Preparations from Lysinibacillus sphaericus Against Culex quinquefasciatus Larvae

Development of Cost Effective Medium for Production of Bacillus sphaericus Strain Isolated from Assam, India

Improvement of Sporulation Conditions of a New Strain of <i>Bacillus amyloliquefaciens</i> in Liquid Fermentation

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Product

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Egg yolk enhances early sporulation and toxicity of Bacillus sphaericus H5a5b for small-scale production of a mosquito control agent

Cited by 3 publications

References 10 publications

Synergistic Activity Between S-Layer Protein and Spore–Crystal Preparations from Lysinibacillus sphaericus Against Culex quinquefasciatus Larvae

Synergistic Activity Between S-Layer Protein and Spore–Crystal Preparations from Lysinibacillus sphaericus Against Culex quinquefasciatus Larvae

Development of Cost Effective Medium for Production of Bacillus sphaericus Strain Isolated from Assam, India

Improvement of Sporulation Conditions of a New Strain of &lt;i&gt;Bacillus amyloliquefaciens&lt;/i&gt; in Liquid Fermentation

Contact Info

Product

Resources

About

Improvement of Sporulation Conditions of a New Strain of <i>Bacillus amyloliquefaciens</i> in Liquid Fermentation