Production of liposomes in a multitubular system useful for scaling up of processes

Carneiro, A. L.; Santana, Maria Helena Andrade

doi:10.1007/b97107

Cited by 6 publications

(6 citation statements)

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“…Although there are reports describing the increase in liposome size, with an increase in the duration of the hydration step for specific methods, to the best of our knowledge, there is not much work explaining the change in size over time at a constant temperature. In their study, Carnerio and Santana explain the change in size by time saying that “The mean diameter increases with time, indicating the aggregation of lipids and a vesiculation phenomenon” . However, this argument is not in agreement with our results as no sign of aggregation or fusion was observed in TEM analysis of the samples after preparation.…”

Section: Resultscontrasting

confidence: 90%

Curvature-Tuned Preparation of Nanoliposomes

2009

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Numerous methods have been reported for the preparation of liposomes, many of which, in addition to requiring time-consuming preparative steps and the use of organic solvents, result in heterogeneous liposome populations of incontrollable size. Taking into consideration the phenomenon of spontaneous vesiculation and the theory of curvature, here we present an extremely rapid and simple, solvent-free method for the preparation of monodisperse solutions of highly stable small unilamellar vesicles using both charged and zwitterionic lipids mixed with lyso-palmitoylphosphatidylcholine, exploiting a combination of a rapid pH change followed by a defined period of equilibration. Various experimental parameters and their interactions were evaluated in terms of their effect on resulting liposome size and shape, as well as on liposome stability and size distribution, with transmission electron microscope imaging being used to visualize the formed liposomes, and photon correlation spectroscopy to obtain statistical data on mean diameter and monodispersity of the liposome population. zeta potential measurements also provided information about the interpretation of vesiculation kinetics and liposome stability. The time interval of pH jump, operation temperature, equilibration time, and lipid type were shown to be the determining factors controlling the size, shape, and monodispersity of the liposomes. Buffer type was also found to be important for the long-term storage of the liposomes. Ongoing work is looking at the application of the developed method for encapsulation of bioactive molecules, such as drugs, genetic materials, and enzymes.

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Section: Resultscontrasting

confidence: 90%

Curvature-Tuned Preparation of Nanoliposomes

2009

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“…A highly efficient method for producing large quantities of drug-encapsulating liposomes rapidly comprises using a continuous flow reactor that can be run indefinitely as long as adequate quantities of reagents are supplied [7] . For these CTRs, a mole balance equation [8] can be used to develop a set of differential equations governing the liposome synthesis reaction.…”

Section: Resultsmentioning

confidence: 99%

Quantitative Modeling of the High-Throughput Production and In Vivo Kinetics of (Drug-Encapsulating) Liposomes

Wong

2010

PLoS ONE

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In developing liposomes for in vivo use, it is important to design the liposomes to have optimal in vivo kinetics, and it is also necessary to identify optimal high-throughput production conditions for these liposomes. Previous work has not definitively established the general relationship between liposomes' configuration and composition, and their in vivo kinetics. Also, no straightforward method exists to calculate optimal liposome high-throughput production conditions for specific liposome compositions. This work presents first-principles quantitative correlations describing liposomes' in vivo drug leakage and vascular mass transfer kinetics. This work further presents a simple quantitative model relating specific liposome compositions to ideal high-throughput production parameters. The results have implications for the identification of promising liposome compositions via high-throughput screening methodologies, as well as the design and optimization of high-throughput reactors for liposome production.

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“…Tournier et al (1999), based on the Bangham method (Bangham et al, 1965), proposed that dried lipid films formed in the inner walls of tubes could be hydrated allowing the self-assembling of lipids generating liposomes. Carneiro and Santana (2004) demonstrated experimentally the potentialities of the liposome production in a multitubular system, useful for scaling up of process. They used neutral lipids, such as soy lecithin, constructed the adsorption isotherm of lipids in the inner wall of glass tubes and monitored the kinetic profiles of liposome generation.…”

Section: Introductionmentioning

confidence: 99%

A mathematical model describing the kinetic of cationic liposome production from dried lipid films adsorbed in a multitubular system

Torre

Carneiro

Rosada

et al. 2007

Braz. J. Chem. Eng.

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Cationic liposomes are an alternative to DNA non-viral carrier in gene therapy, promoting higher efficiency for transport and delivery into the cells. Liposome production large scale is still a challenge. Among many processes, from dried film adsorbed inside multitubular system promising due its operational simplicity. this field, engineering contributions scarce no mathematical treatment literature describing type of process available. formulation proposed describe kinetic considering wetting disintegration lipid followed by lipids aggregation bilayers generating liposomes. model has fitted experimental data mass thickness, being useful predictions scaling up as well contributing development pharmaceutical products

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Production of liposomes in a multitubular system useful for scaling up of processes

Cited by 6 publications

References 0 publications

Curvature-Tuned Preparation of Nanoliposomes

Curvature-Tuned Preparation of Nanoliposomes

Quantitative Modeling of the High-Throughput Production and In Vivo Kinetics of (Drug-Encapsulating) Liposomes

A mathematical model describing the kinetic of cationic liposome production from dried lipid films adsorbed in a multitubular system

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