Production of Lipids Containing High Levels of Docosahexaenoic Acid by a Newly Isolated Microalga, Aurantiochytrium sp. KRS101

Abstract

In the present study, a novel oleaginous Thraustochytrid containing a high content of docosahexaenoic acid (DHA) was isolated from a mangrove ecosystem in Malaysia. The strain identified as an Aurantiochytrium sp. by 18S rRNA sequencing and named KRS101 used various carbon and nitrogen sources, indicating metabolic versatility. Optimal culture conditions, thus maximizing cell growth, and high levels of lipid and DHA production, were attained using glucose (60 g l⁻¹) as carbon source, corn steep solid (10 g l⁻¹) as nitrogen source, and sea salt (15 g l⁻¹). The highest biomass, lipid, and DHA production of KRS101 upon fed-batch fermentation were 50.2 g l⁻¹ (16.7 g l⁻¹ day⁻¹), 21.8 g l⁻¹ (44% DCW), and 8.8 g l⁻¹ (40% TFA), respectively. Similar values were obtained when a cheap substrate like molasses, rather than glucose, was used as the carbon source (DCW of 52.44 g l⁻¹, lipid and DHA levels of 20.2 and 8.83 g l⁻¹, respectively), indicating that production of microbial oils containing high levels of DHA can be produced economically when the novel strain is used.

Microalgal Oil Supplementation Has an Anti-Obesity Effect in C57BL/6J Mice Fed a High Fat Diet

“…Beef tallow and corn oil were purchased from the Ottogi Company (Seoul, Korea). Microalgal oil was obtained from Korea Research Institute of Bioscience and Biotechnology (KRIBB, Jeongeup, Korea); the oil from the microalgae was extracted by using a previously reported method (12,15). The animals were housed in a temperature-controlled environment with a 12-h light/dark cycle and free access to food and water during the entire experimental period.…”

“…Among these, microalgal oil is an attractive alternative for a substantial number of reasons. Microalgae, the common name for multiple single-cell, photogenic, heterotrophic, and mixotrophic organisms, have a high growth rate, short life cycle, and are less affected by environmental conditions such as season, location, and climate (6,12). Several species of microalgae can be stimulated for overproduction of a specific fatty acid, including EPA or DHA, through relative simple manipulations of their culture medium (13).…”

“…KRS101 is an oleaginous heterotrophic microalga found in the Malaysian mangrove ecosystem like soil, leaf and pneumatophore samples. These microalgae are capable of producing high quantity of lipid with DHA content over 45% of total fatty acid (12) which is safe for use in food and feed industries (15). Many studies indicate that microalgal oils improve lipoprotein profiles; however these studies did not show any beneficial effect in terms of obesity (16)(17)(18).…”

Opportunities to enhance alternative sources of long-chainn-3 fatty acids within the diet

“…Examples of micro-organisms are microalgae (25)(26)(27) including thraustochytrids (28,29) and Escherichia coli (30) .…”

Prevalence of PUFA Rich Thraustochytrids sps. along the Coast of Mumbai for Production of Bio Oil

“…The samples were then heated at 90°C for 1 h in sealed vials. Fatty acid methyl esters (FAMEs) were then extracted into 0.6 ml hexane and analyzed by gas chromatography (GC; Hewlett Packard 6890N) using Nitrogen as a carrier gas (Hong et al, 2011). The instrument was equipped with a flameionization detector (FID) and an SP 2300 column having length of 2m and internal diameter of 2 mm.…”