Production of lipase by a newly isolated Bacillus coagulans under solid-state fermentation using melon wastes

Abstract

An extracellular lipase was produced by Bacillus coagulans by solid-state fermentation. Solid waste from melon was used as the basic nutrient source and was supplemented with olive oil. The highest lipase production (78,069 U/g) was achieved after 24 h of cultivation with 1% olive oil enrichment. Enzyme had an optimal activity at 37 degrees C and pH 7.0, and sodium dodecyl sulfate increased lipase activity. NH4NO3 increased enzyme production, whereas organic nitrogen had no effect. The effect of the type of carbon sources on lipolytic enzyme production was also studied. The best results were obtained with starch and maltose (148,932 and 141,629 U/g, respectively), whereas a rather low enzyme activity was found in cultures grown on glucose and galactose (approx 118,769 and 123,622 U/g, respectively). Enzyme was inhibited with Mn+2 and Ni+2 by 68 and 74%, respectively. By contrast, Ca+2 enhanced enzyme production by 5%.

A Review on Microbial Lipases Production

“…Mahanta et al (2008) obtained a maximum lipase activity of 1,084 U gds −1 using a solvent tolerant P. aeruginosa PseA strain. Alkan et al (2007) produced an extracellular lipase by B. coagulans and obtained a maximum lipase activity of 149 U gds −1 after 24 h of fermentation. Fernandes et al (2007) obtained a maximum lipase activity of 108 U gds −1 after 72 h of fermentation by B. cepacia.…”

Purification and Physicochemical Properties of Lipase from Thermophilic Bacillus aerius

“…Also, lipases do not require cofactors to catalyse hydrolytic reactions and remain active in the presence of organic solvents 5 . Bacterial lipases are produced by both submerged and solid-state fermentation 6,7 . Lipase production requires carbon and nitrogen sources as required by any fermentation process.…”

Characterization of Aspergillus Niger 65i6 lipase from solid-state fermentation using Jatropha seed cake medium

“…Plant lipase has been produced from Jatropha seeds (Abigor, 2002;Hidayat et al, 2014), palm oil (Ebongue et al, 2006), and rice bran (Chong et al, 2007;Hidayat et al, 2014). Bacterial lipases have been obtained from Bacillus coagulans and Pseudomonas aeruginosa (Alkan, 2007;Mahanta et al, 2008). Meanwhile, yeast lipase has been produced mostly from Candida rugosa and Candida antarctica (Bussamara, 2010).…”