Production of Lignocellulytic Enzymes from Spent Mushroom Compost of Pleurotus eryngii

Lim, Sun-Hwa; Kim, Jong-Kun; Lee, Yun-Hae; Kang, Hee‐Wan

doi:10.4489/kjm.2012.40.3.152

Cited by 9 publications

(3 citation statements)

References 15 publications

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“…3 ). A previous study showed that the SMC of P. eryngii can digest FP [ 15 ] and the results indicated that the enzymes extracted from the SMC of Pleurotus have industrial value. Thus, the results of this study showing that the SMC extracts of P. cornucopiae and P. eryngii can digest FP suggest that SMC can be used in production of bio-energy by degradation of biomass.…”

Section: Resultsmentioning

confidence: 99%

Efficient Recovery of Lignocellulolytic Enzymes of Spent Mushroom Compost from Oyster Mushrooms, Pleurotus spp., and Potential Use in Dye Decolorization

2013

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This study was conducted in order to perform efficient extraction of lignocellulolytic enzymes amylase (EC 3.2.1.1), cellulase (EC 3.2.1.4), laccase (EC 1.10.3.2), and xylanase (EC 3.2.1.8) from spent mushroom compost (SMC) of Pleurotus ostreatus, P. eryngii, and P. cornucopiae. Optimal enzyme recovery was achieved when SMCs were extracted with 50 mM sodium citrate (pH 4.5) buffer at 4℃ for 2 hr. Amylase, cellulase, and xylanase activities showed high values in extracts from P. ostreatus SMC, with 2.97 U/g, 1.67 U/g, and 91.56 U/g, respectively, whereas laccase activity and filter paper degradation ability were highest in extracts from P. eryngii SMC, with values of 9.01 U/g and 0.21 U/g, respectively. Enzymatic activities varied according to the SMCs released from different mushroom farms. The synthetic dyes remazol brilliant blue R and Congo red were decolorized completely by the SMC extract of P. eryngii within 120 min, and the decolorization ability of the extract was comparable to that of 0.3 U of commercial laccase. In addition, laccase activity of the SMC extract from P. eryngii was compared to that of commercial enzymes or its industrial application in decolorization.

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Section: Resultsmentioning

confidence: 99%

Efficient Recovery of Lignocellulolytic Enzymes of Spent Mushroom Compost from Oyster Mushrooms, Pleurotus spp., and Potential Use in Dye Decolorization

2013

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“…This phenomenon was in accordance with growth profiles of fungal mycelia. Basidiomycetes, such as the oyster mushroom, king oyster mushroom, winter mushroom, and shiitake, can enzymatically degrade cultivation substrates containing lignin, hemicellulose, and cellulose into low molecular weight sugars, which are then absorbed by fungal hyphae to support growth and maturation in a process called nutritive absorption (Lim et al, 2012, 2013; Chen et al, 2016). In the late stage of mycelial maturation, the slowing of metabolism causes a decrease in the levels of extracellular enzymes, so the abundance of degradation products is correspondingly reduced.…”

Section: Discussionmentioning

confidence: 99%

Metabolic Profiling of Pleurotus tuoliensis During Mycelium Physiological Maturation and Exploration on a Potential Indicator of Mycelial Maturation

Zou

et al. 2019

Front. Microbiol.

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Pleurotus tuoliensis is a valuable and rare edible fungus with extremely high nutritional and medicinal value. However, the relative immaturity of P. tuoliensis cultivation technology leads to fluctuating yields and quality. The main difficulty in P. tuoliensis cultivation is estimate of mycelial maturity. There is currently no measurable indicator that clearly characterizes the physiological maturation of mycelia. The aim of this study was to identify potential indicators of physiological maturation for P. tuoliensis mycelia by using metabolomics approach. A metabolite profiling strategy involving gas chromatography-mass spectrometry (GC/MS) was used to analyze changes to extracellular metabolites in mycelia collected at mycelium physiological maturation period (MPMP) day 0, MPMP day 35 at 17°C and MPMP day 35 at 29°C. 72 differential metabolites (37.8% up-regulated and 62.2% down-regulated) were identified based on the selected criteria [variable important in projection (VIP) greater than 1.0 and p < 0.01]. Metabolic pathways enrichment analysis showed that these metabolites are involved in glycolysis, organic acid metabolism, amino acid metabolism, tricarboxylic acid cycle (TCA), sugar metabolism, nicotinate and nicotinamide metabolism, and oxidative phosphorylation. In addition, the pyrimidine synthesis pathway was significantly activated during mycelium physiological maturation of P. tuoliensis. The abundance of N-carbamoyl-L-aspartate (CA-asp), a component of this pathway, was significantly increased at MPMP day 35, which motivated us to explore its potential as an indicator for physiological maturation of mycelia. The content of CA-asp in mycelia changed in a consistent manner during physiological maturation. The feasibility of using CA-asp as an indicator for mycelial maturation requires further investigation.

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“…The first studies were published in the early 2000s, when different solvent systems, including water, sodium citrate buffer, and sodium phosphate buffer, were evaluated to extract hydrolases and oxidoreductases from SMS of P. sajor-caju SMS [ 170 ] and P. ostreatus [ 171 ], the latter also included SMS of other species. In other studies, different buffers and conditions were evaluated for extraction of α-amylase, endoglucanase, laccase, and endoxylanase from SMS of P. eryngii, P. ostreatus , and P. cornucopiae ; the best recoveries were achieved using sodium citrate buffer [ 172 , 173 ]. Sadiq et al [ 174 ] used a sodium tartrate buffer for extracting manganese peroxidase (MnP), laccase, and lignin peroxidase (LiP) from P. ostreatus SMS and used the extract for bioremediation of contaminated soil.…”

Section: Spent Mushroom Substrate As Source Of Enzymes and Bioactive ...mentioning

confidence: 99%

Spent substrate from mushroom cultivation: exploitation potential toward various applications and value-added products

Martín,

Zervakis,

Xiong

et al. 2023

Bioengineered

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Spent mushroom substrate (SMS) is the residual biomass generated after harvesting the fruitbodies of edible/medicinal fungi. Disposal of SMS, the main by-product of the mushroom cultivation process, often leads to serious environmental problems and is financially demanding. Efficient recycling and valorization of SMS are crucial for the sustainable development of the mushroom industry in the frame of the circular economy principles. The physical properties and chemical composition of SMS are a solid fundament for developing several applications, and recent literature shows an increasing research interest in exploiting that inherent potential. This review provides a thorough outlook on SMS exploitation possibilities and discusses critically recent findings related to specific applications in plant and mushroom cultivation, animal husbandry, and recovery of enzymes and bioactive compounds.

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Production of Lignocellulytic Enzymes from Spent Mushroom Compost of Pleurotus eryngii

Cited by 9 publications

References 15 publications

Efficient Recovery of Lignocellulolytic Enzymes of Spent Mushroom Compost from Oyster Mushrooms, Pleurotus spp., and Potential Use in Dye Decolorization

Efficient Recovery of Lignocellulolytic Enzymes of Spent Mushroom Compost from Oyster Mushrooms, Pleurotus spp., and Potential Use in Dye Decolorization

Metabolic Profiling of Pleurotus tuoliensis During Mycelium Physiological Maturation and Exploration on a Potential Indicator of Mycelial Maturation

Spent substrate from mushroom cultivation: exploitation potential toward various applications and value-added products

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