Production of Lignocellulolytic Enzymes and Biomass of Trametes versicolor from Agro-Industrial Residues in a Novel Fixed-Bed Bioreactor with Natural Convection and Forced Aeration at Pilot Scale

Abstract

Solid-state fermentation requires the development of more efficient cultivation systems for its industrial application. The objective of this work was to evaluate the effect of aeration regime on the production of biomass and several lignocellulolytic enzymes (laccase, manganese peroxidase, endoxylanase, β-glucosidase, and total cellulases) by Trametes versicolor in a novel fixed-bed solid-state pilot-scale bioreactor. Three regimes were assessed: natural convection, low aeration level (10 min every 6 h at 10 L/h air flowrate), and high aeration level (1 h every 6 h at 10 L/h air flowrate). The mushroom was grown on a medium based on lignocellulosic residues. The design of the bioreactor, as well as the control of aeration, humidity, and temperature of the beds, allowed T. versicolor to grow properly on the medium, reaching a maximum biomass production of 204.7 mg/g dry solid (ds). The influence that aeration regime had on the production of lignocellulolytic enzymes was determined. Low level of forced aeration favored obtaining the highest titers of laccase (6.37 U/g ds) compared to natural convection and high aeration level. The greatest lignin degradation was also verified for low aeration regime. For the first time, pilot scale cultivation of T. versicolor was reported in a fixed-bed bioreactor.

Yellow laccase produced by Trametes versicolor K1 on tomato waste: A comparative study with the blue one produced on semi-synthetic medium

“…According to this, TMT supplied with peptone was used to produce laccase by T. versicolor K1. Laccase activity was not increased in a 1 L culture volume; factors like aeration, humidity, and mixing need to be investigated to increase laccase production in large volumes of TMT medium (Meilany et al, 2020;Montoya et al, 2021). However, it recorded higher laccase activity compared to T. versicolor and P. ostreatus laccases produced on a 2 % tomato pomace medium supplemented with a miniral solution (Freixo et al, 2008b(Freixo et al, , 2008a.…”

Solid-state fermentation: a review of its opportunities and challenges in the framework of circular bioeconomy

“…Temperature control in SSF is normally achieved by convection through forced-aeration to increase heat dissipation and to avoid undesired temperature gradients. However, forced aeration also provokes moisture losses and drying, therefore, it must be carefully controlled [29]. In this sense, the configuration of novel SSF bioreactors, with innovative aeration and cooling strategies (including irrigation, mixing, etc.)…”

Cultural and Morphological Features of Trametes versicolor (Polyporaceae) Growth on Wood Hydrolyzes Containing Media

“…Дереворуйнівні макроміцети, зокрема T. versicolor, у природних умовах ростуть на листяних деревах (березі, буці, вербі, дубі тощо), іноді трапляються на хвойних (модрині, ялині, сосні) [39,40]. Зазвичай дослідники відштовхуються від природних субстратів і для отримання плодових тіл чи ферментів T. versicolor культивують на дубовій, буковій, грабовій, березовій чи іншій тирсі, до якої можуть додавати мінеральні компоненти для підвищення виходу цільового продукту [41][42][43]. Екстракти тирси дерев, отримані з використанням різних підхо-дів, містять в основному гексози, пентози, моно-та поліфенольні сполуки, мінерали тощо.…”