Yellow laccase produced by Trametes versicolor K1 on tomato waste: A comparative study with the blue one produced on semi-synthetic medium

Chaoua, Samah; Chaouche, Noreddine Kacem; Songulashvili, George; Gares, Maroua; Hiligsmann, Serge; Flahaut, Sigrid

doi:10.1016/j.jbiotec.2022.12.001

Cited by 6 publications

(6 citation statements)

References 48 publications

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“…Based on the factors affecting the secretion of laccase by T. versicolor and the preliminary experiment results (data not shown), 50,51 fermentation time, fermentation temperature, moisture content of the corn substrate and inoculation amount were selected as the major factors affecting AFB 1 degradation. The AFB 1 degradation rate was used as the evaluation index for single‐factor experiments.…”

Section: Methodsmentioning

confidence: 99%

“…The activity of laccase was expressed as U per gram of dry fermentation product (U g −1 ). 49 Single-factor experiments on the degradation of AFB 1 in corn Based on the factors affecting the secretion of laccase by T. versicolor and the preliminary experiment results (data not shown), 50,51 fermentation time, fermentation temperature, moisture content of the corn substrate and inoculation amount were selected as the major factors affecting AFB 1 degradation.…”

Section: Determination Of Laccase Activitymentioning

confidence: 99%

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Optimizing the degradation of aflatoxin B₁ in corn by Trametes versicolor and improving the nutritional composition of corn

Lou,

Li,

Yang

et al. 2023

J Sci Food Agric

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BackgroundCorn, being an important grain, is prone to contamination by aflatoxin B1 (AFB1), and AFB1‐contaminated corn severely endangers the health of humans and livestock. Trametes versicolor, a fungus that can grow in corn, possesses the ability to directly degrade AFB1 through its laccase. This study aimed to optimize the fermentation conditions for T. versicolor to degrade AFB1 in corn and investigate the effect of T. versicolor fermentation on the nutritional composition of corn. AFB1‐contaminated corn was used as the culture substrate for T. versicolor. A combination of single factor experiments and response surface methodology was employed to identify the optimal conditions of AFB1 degradation.ResultsThe results showed that the optimal conditions of AFB1 degradation were as follows: 9 days of fermentation, a fermentation temperature of 26.7 °C, a moisture content of 70.5%, and an inoculation amount of 4.9 mL (containing 51.99 mg of T. versicolor mycelia). With the optimal conditions, the degradation rate of AFB1 in corn could reach 93.01%, and the dry basis content of protein and dietary fiber in the fermented corn was significantly increased. More importantly, the lysine content in the fermented corn was also significantly increased.ConclusionThis is the first report that direct fermentation of AFB1‐contaminated corn by T. versicolor not only efficiently degrades AFB1 but also improves the nutritional composition of corn. These findings suggest that the fermentation of corn by T. versicolor is a promising, environmentally friendly, and efficient approach to degrade AFB1 and improve the nutritional value of corn.This article is protected by copyright. All rights reserved.

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Section: Methodsmentioning

confidence: 99%

Section: Determination Of Laccase Activitymentioning

confidence: 99%

Optimizing the degradation of aflatoxin B₁ in corn by Trametes versicolor and improving the nutritional composition of corn

Lou,

Li,

Yang

et al. 2023

J Sci Food Agric

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“…It contains skins, seeds, and some peels. White rot fungus T. versicolor K1 (ON970954) was identified at BCCM/MUCL (Belgium) after being isolated from the bark of a date palm, in Algeria [14]. The strain is deposited at the BCCM/MUCL public collection (Belgium) with the identifier MUCL 58308.…”

Section: Lignocellulosic Biomass Microorganism and Preculturesmentioning

confidence: 99%

“…Non-inoculated blanks of TW and PtTW were incubated under the same conditions. At the end of fermentation, the culture supernatants were centrifuged for 15 min at 10,000 rpm and at 4 °C before being passed through a 0.22-μm porosity filter and stored at 4 °C for further use [14].…”

Section: Submerged Fermentation (Smf)mentioning

confidence: 99%

“…The crude laccase (TmtLac) obtained, previously, from TW submerged culture (5%) by T. versicolor K1 was used to treat dried TW, and PtTW [14]. Laccase treatment was performed in hermetically sealed tubes placed in a stirred bath (150 rpm, at 50 °C) at 5% (w/v) dried biomass load in a 50 mM sodium acetate buffer pH 4.8 for 24 h. The treatment was carried out using 100 U/g of substrate without the presence of a mediator [17].…”

Section: Laccase Treatmentmentioning

confidence: 99%

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Assessment of Green Processes for Tomato Waste Biovalorization: Spotlight on the Innovative Pulsed Electric Field–Laccase Synergy for Enhanced Sugar and Phenol Extraction Yields

Chaoua,

Flahaut,

Hiligsmann

et al. 2023

Bioenerg. Res.

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Tomato waste (TW) is a plentiful lignocellulosic resource, mainly composed of seeds and skins, that can be converted into high-value compounds. This study explored the enhancement of TW enzymatic and fungal biovalorization using novel ecofriendly approaches, including advanced technology, pulsed electric fields (PEF). Crude laccase first produced on TW was used for enzymatic treatment, and the white rot fungus Trametes versicolor K1 was used in fungal treatment under SmF (submerged) or SSF (semi-solid) fermentation conditions. The physical PEF treatment had increased tenfold sugar extraction yield (83.4 mg/g) and twofold polyphenol extraction yield (4.43 g/g), with respect to the control. PEF-laccase innovative combination, reported for the first time, has enhanced significantly sugar extraction yield (100.6 mg/g), twofold higher than those released from TW after laccase treatment alone. However, the PEF treatment had no effect on polyphenol extraction yield when combined to laccase or fungal treatments. The treated TW was subjected to polysaccharide enzymatic hydrolysis. The combination of PEF with laccase or fungal treatment did not impact sugar yields; however, it allowed polyphenol liberation. During fungal treatment (i.e., T. versicolor K1 grown on TW), comparable maximal laccase activities of 2574.28 U/L and 2577.06 U/L were measured in the culture supernatants, in SmF and SSF conditions, respectively. The findings demonstrate the high potential of PEF for recovering phenols and sugars. When combined to fungal treatment, it offers high yields of valuable products, making it a potential cost-effective approach, providing new prospects for TW valorization.

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Unlocking the potential of Algerian lignocellulosic biomass: exploring indigenous microbial diversity for enhanced enzyme and sugar production

Chaoua,

Flahaut,

Cornu

et al. 2024

Arch Microbiol

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Yellow laccase produced by Trametes versicolor K1 on tomato waste: A comparative study with the blue one produced on semi-synthetic medium

Cited by 6 publications

References 48 publications

Optimizing the degradation of aflatoxin B₁ in corn by Trametes versicolor and improving the nutritional composition of corn

Optimizing the degradation of aflatoxin B₁ in corn by Trametes versicolor and improving the nutritional composition of corn

Assessment of Green Processes for Tomato Waste Biovalorization: Spotlight on the Innovative Pulsed Electric Field–Laccase Synergy for Enhanced Sugar and Phenol Extraction Yields

Unlocking the potential of Algerian lignocellulosic biomass: exploring indigenous microbial diversity for enhanced enzyme and sugar production

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Yellow laccase produced by Trametes versicolor K1 on tomato waste: A comparative study with the blue one produced on semi-synthetic medium

Cited by 6 publications

References 48 publications

Optimizing the degradation of aflatoxin B1 in corn by Trametes versicolor and improving the nutritional composition of corn

Optimizing the degradation of aflatoxin B1 in corn by Trametes versicolor and improving the nutritional composition of corn

Assessment of Green Processes for Tomato Waste Biovalorization: Spotlight on the Innovative Pulsed Electric Field–Laccase Synergy for Enhanced Sugar and Phenol Extraction Yields

Unlocking the potential of Algerian lignocellulosic biomass: exploring indigenous microbial diversity for enhanced enzyme and sugar production

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Optimizing the degradation of aflatoxin B₁ in corn by Trametes versicolor and improving the nutritional composition of corn

Optimizing the degradation of aflatoxin B₁ in corn by Trametes versicolor and improving the nutritional composition of corn