Production of lactulose oligosaccharides by isomerisation of transgalactosylated cheese whey permeate obtained by β-galactosidases from dairyKluyveromyces

Abstract

β-Galactosidases from Kluyveromyces lactis and Kluyveromyces marxianus isolated from artisanal ewes' milk cheeses, were used to transgalactosylate lactose from cheese whey permeate (WP). The content of galactooligosaccharides (GOS) obtained by transgalactosylation was comparable with that formed using pure lactose as substrate. In order to obtain a mixture with higher prebiotic oligosaccharide content, isomerisation of the transgalactosylated WP was carried out using sodium aluminate as catalyst. The transgalactosylated mixtures at 6 h of reaction contained amounts of prebiotic carbohydrates (tagatose, lactulose, GOS and oligosaccharides derived from lactulose, OsLu) close to 50 g/100 g of total carbohydrates for all the strains tested, corresponding to 322 g prebiotics/kg whey permeate. Thus, the suitability of this methodology to produce mixtures of dietary non-digestible carbohydrates with prebiotic properties from WP has been demonstrated, which is interesting for the food industry since it increases the value and the applicability of this by-product from cheese manufacture.

Microbial Glycosidases for Nondigestible Oligosaccharides Production

“…A high lactose conversion was achieved (70-80%), when using whey as a substrate in the production of GOs, yielding 10-20% of total sugars and producing oligomers with DP3, DP4, and DP5 [16]. The GOs production from whey permeate yielded 50% corresponding to 322 g prebiotics/kg whey permeate, presenting tagatose and lactulose in the oligosaccharides mixture [17]. Galacto-oligosaccharides were synthesized by enzymatic transgalactosylation in UF-skimmed milk permeate fortified with lactose (40% w/w).…”

Synthesis of Galactooligosaccharides in Milk and Whey: A Review

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An efficient process for obtaining prebiotic oligosaccharides derived from lactulose using isomerized and purified whey permeate

“…Other prebiotic oligosaccharides obtained from WP are those derived from lactulose (OsLu), which have been synthesized by chemo‐enzymatic methodologies by combination of chemical isomerization of lactose, using egg shell as catalyst, and subsequent transglycosylation of the resulting mixture using β‐galactosidase from Bacillus circulans . Moreover, OsLu have been produced by isomerization using sodium aluminates as catalyst, of transglycosylated mixtures previously obtained by hydrolysis and transglycosylation of lactose standard or from WP, using β‐galactosidases from dairy Kluyveromyces lactis and K. marxianus …”

“…11 Moreover, OsLu have been produced by isomerization using sodium aluminates as catalyst, of transglycosylated mixtures previously obtained by hydrolysis and transglycosylation of lactose standard or from WP, using -galactosidases from dairy Kluyveromyces lactis and K. marxianus. 12,13 A wide number of catalysts, such as sodium and calcium hydroxide, complexation reagents (aluminates and borates), zeolites, sepiolites, egg and oyster shell, 11,14 -18 have been used to isomerize lactose. The use of aluminates or borates enables yields up to 80% of lactulose.…”