Production of l-xylulose from xylitol by a newly isolated strain of Bacillus pallidus Y25 and characterization of its relevant enzyme xylitol dehydrogenase

Facile Enzymatic Synthesis of Ketoses

“…Chemical methods for the synthesis of these rare ketoses require tedious protection/deprotection manipulations. [10] Alternatively, enzymatic preparation by epimerizing ketoses at the C-3 position, [11] oxidizing polyols, [12] or relying on aldol condensation [13] proceeds regio-and stereoselectively without protection. However, these processes suffer from low yields, expensive starting materials or a complicated isomer separation step.…”

Polyol Conversion Specificity ofBacillus pallidus

“…4,5) In this study, we observed the production of elusive rare sugars, including L-talose, L-glucose, and L-mannose from L-talitol, L-mannitol, and L-mannitol respectively, and L-talitol and L-mannitol were produced by reverse reaction from L-tagatose and L-fructose respectively (Fig. 2).…”

“…Allitol and xylitol have been found to be the main substrates of B. pallidus, followed by D-iditol, L-arabitol, ribitol, D-arabitol, and galactitol. 4,5) In this report, we describe the structural dependency of sugar substrates in the cellular reaction of this bacterium due to -OH configurations at C2 and C3 positions of polyols, and its potential application in the production of various rare sugars.…”

“…Recently, we isolated Bacillus pallidus Y25, a novel, moderately thermophilic bacterium capable of producing rare ketoses from polyols. 4) This bacterium showed broad substrate specificity, oxidizing various polyols to their corresponding ketoses. Allitol and xylitol have been found to be the main substrates of B. pallidus, followed by D-iditol, L-arabitol, ribitol, D-arabitol, and galactitol.…”

“…[1][2][3][4][5] In addition, bioconversion strategies for all rare sugars have been schematized. 6) Biotransformation is generally recognized as environmentally friendly compared to other chemical processes, and it is economically competitive in terms of cost and productivity.…”