Polyol Conversion Specificity of<i>Bacillus pallidus</i>

Poonperm, Wayoon; Takata, Goro; Izumori, Ken

doi:10.1271/bbb.70475

Cited by 16 publications

(6 citation statements)

References 12 publications

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“…These rare sugars play crucial roles as recognition elements in bioactive molecules [1,2]. They have a wide range of uses, from sweeteners to functional foods and potential therapeutics [3].…”

Section: Introductionmentioning

confidence: 99%

Repeated production of l-xylulose by an immobilized whole-cell biocatalyst harboring l-arabinitol dehydrogenase coupled with an NAD+ regeneration system

Gao

Kim

Choi³

et al. 2015

Biochemical Engineering Journal

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Section: Introductionmentioning

confidence: 99%

Repeated production of l-xylulose by an immobilized whole-cell biocatalyst harboring l-arabinitol dehydrogenase coupled with an NAD+ regeneration system

Gao

Kim

Choi³

et al. 2015

Biochemical Engineering Journal

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“…For an enzyme able to sense the presence of sugar alcohols in situ, we selected ribitol dehydrogenase (RDH) to encapsulate into the cavity of polymersomes. [38,39] As we had already demonstrated the functional reconstitution of a channel porin OmpF in polymersome membranes [40], we initially produced PNRs by self-assembly of PMOXA6-b-PDMS42-b-PMOXA6 in the presence of OmpF and RDH, in order to optimize conditions for encapsulating the enzyme. OmpF-PNRs were compared with empty polymersomes, and with unpermeabilised nanoreactors (PNRs without OmpF).…”

Section: Strategy For Creating "Active Surfaces" Based On Pnrsmentioning

confidence: 99%

“…conversion of ribitol with oxidized nicotinamide adenine dinucleotide (NAD + ) to D-ribulose, and reduced nicotinamide adenine dinucleotide (NADH). [38,39] Therefore this "smart" combination of biomolecules serves to obtain a selective transport through the membrane of sugar alcohols, and their detection, as key point of the biosensor selectivity.…”

Section: Introductionmentioning

confidence: 99%

Active surfaces engineered by immobilizing protein-polymer nanoreactors for selectively detecting sugar alcohols

et al. 2016

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We introduce active surfaces generated by immobilizing protein-polymer nanoreactors on a solid support for sensitive sugar alcohols detection. First, such selective nanoreactors were engineered in solution by simultaneous encapsulation of specific enzymes in copolymer polymersomes, and insertion of membrane proteins for selective conduct of sugar alcohols. Despite the artificial surroundings, and the thickness of the copolymer membrane, functionality of reconstituted Escherichia coli glycerol facilitator (GlpF) was preserved, and allowed selective diffusion of sugar alcohols to the inner cavity of the polymersome, where encapsulated ribitol dehydrogenase (RDH) enzymes served as biosensing entities. Ribitol, selected as a model sugar alcohol, was detected quantitatively by the RDH-nanoreactors with GlpF-mediated permeability in a concentration range of 1.5-9 mM. To obtain "active surfaces" for detecting sugar alcohols, the nanoreactors optimized in solution were then immobilized on a solid support: aldehyde groups exposed at the compartment external surface reacted via an aldehyde-amino reaction with glass surfaces chemically modified with amino groups. The nanoreactors preserved their architecture and activity after immobilization on the glass surface, and represent active biosensing surfaces for selective detection of sugar alcohols, with high sensitivity.

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“…Although D-altritol is not known to be a widespread plant metabolite, polyols are primary photosynthetic products; therefore, the existence of D-altritol may be phylogenetically widespread, albeit at low abundance (22). Notably, Izumori and co-workers (23)(24)(25)(26) have shown that various organisms, including fungi, yeast, and bacteria, have the capability to synthesize D-altritol, although the pathway has not been studied.…”

Section: Innovations In the Discovery Of The Functions Of Uncharactermentioning

confidence: 99%

ATP-binding Cassette (ABC) Transport System Solute-binding Protein-guided Identification of Novel d-Altritol and Galactitol Catabolic Pathways in Agrobacterium tumefaciens C58

Wichelecki

Vetting

Chou

et al. 2015

Journal of Biological Chemistry

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Background: Solute-binding proteins for ABC transport systems can be used to guide discovery of novel enzymes and metabolic pathways. Results: Novel metabolic pathways were discovered for D-altritol and galactitol catabolism. Conclusion: More than 2,000 proteins with previously unknown functions were annotated using this strategy. Significance: Strategies are described that can be used to address misannotation in protein databases.

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Polyol Conversion Specificity ofBacillus pallidus

Cited by 16 publications

References 12 publications

Repeated production of l-xylulose by an immobilized whole-cell biocatalyst harboring l-arabinitol dehydrogenase coupled with an NAD+ regeneration system

Repeated production of l-xylulose by an immobilized whole-cell biocatalyst harboring l-arabinitol dehydrogenase coupled with an NAD+ regeneration system

Active surfaces engineered by immobilizing protein-polymer nanoreactors for selectively detecting sugar alcohols

ATP-binding Cassette (ABC) Transport System Solute-binding Protein-guided Identification of Novel d-Altritol and Galactitol Catabolic Pathways in Agrobacterium tumefaciens C58

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