Production of keratinolytic enzyme by a newly isolated feather-degrading Stenotrophomonas maltophilia that produces plant growth-promoting activity

Jeong, Jin-Ha; Lee, O-Mi; Jeon, Young-Dong; Kim, Jeong-Do; Lee, Na-Ri; Lee, Chung-Yeol; Son, Hong-Joo

doi:10.1016/j.procbio.2010.07.020

Cited by 95 publications

(88 citation statements)

References 35 publications

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“…IAA production has been (Kochar et al 2011) and indole-3-acetamide-mediated IAA biosynthesis by Fusarium species (Tsavkelova et al 2012). However, very few reports are available regarding IAA production through feather degradation (Jeong et al 2010a). The hydrolysis of feathers results in a significant amount of l-tryptophan (Jeong et al 2010a) and most of the keratinolytic microorganisms having the ability to produce IAA from l-tryptophan.…”

Section: Discussionmentioning

confidence: 99%

“…Aliquots of 2 ml were retrieved from each triplicate culture after every 24 h to determine the concentration of IAA. The supernatant (1 ml) of grown culture was mixed with 500 µl of Salkowski reagent (2 % of 0.5 M FeCl 3 in 35 % perchloric acid) and the concentration of IAA was estimated after 20 min by measuring the absorbance at 530 nm (Jeong et al 2010a). The absorbance was compared with standard prepared by using IAA (HiMedia, India).…”

Section: Study Of Iaa and Ammonia Productionmentioning

confidence: 99%

“…Preparation of biofertilizers using chicken feather wastes is attracting the focus of many research scientists. Feather meal is a cheap and easily available source of nitrogen (15 % N) and may serve as a potential biofertilizer (Jeong et al 2010a). The plant growth-promoting activity of protein hydrolysates could also be effectively applied in agriculture (Tiwary and Gupta 2010).…”

Section: Introductionmentioning

confidence: 99%

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Ameliorating effects of chicken feathers in plant growth promotion activity by a keratinolytic strain of Bacillus subtilis PF1

Bhange

Chaturvedi

Bhatt

2016

Bioresour. Bioprocess.

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Background: Feathers are the major byproducts of poultry industry and considered as waste. Feathers (composed of protein keratin) are metabolized by a number of microorganisms as a source of carbon and nitrogen. Degradation of feathers results in production of amino acids and peptides, which can be employed as precursors for plant growthpromoting metabolites such as indole acetic acid, ammonia and HCN. The aim of the present investigation was to assess the influence of these metabolites (termed as feather protein hydrolysate) on plant growth promotion activity of keratinolytic bacterial strain Bacillus subtilis PF1.Results: Strain PF1 exhibits potent keratinolytic activity and can efficiently degrade 10 g/l chicken feathers under submerged cultivation with 81.4 ± 4.40 U/ml keratinase activity. Different concentrations of feathers supported the production of indole acetic acid by strain PF1. Strain PF1 produces maximum indole acetic acid (46.2 ± 0.21 µg/ml) in the presence of 2.0 % feathers at 120 h of incubation. The indole acetic acid production was confirmed by thin-layer chromatography and Fourier transform infrared spectroscopic analysis. However, increased concentration of feathers exhibited negative effect on phosphate solubilization due to increased alkalinity. HCN production also exhibited positive correlation with concentration of feathers. Finally, plant growth of Vigna radiata in the presence of strain PF1 with chicken feathers in soil was investigated, which showed good plant growth promotion activity. Increased ratio of C/N in soil also supported the plant growth promotion activity of feathers. Conclusion:Feather degradation property of B. subtilis PF1 could be efficiently utilized for feather waste management. The metabolites released by feather degradation along with strain PF1 could be successfully employed as an economic source of nitrogen fertilizers for plants.

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Section: Discussionmentioning

confidence: 99%

Section: Study Of Iaa and Ammonia Productionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Ameliorating effects of chicken feathers in plant growth promotion activity by a keratinolytic strain of Bacillus subtilis PF1

Bhange

Chaturvedi

Bhatt

2016

Bioresour. Bioprocess.

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“…In particular, keratinases from B. licheniformis are capable in degrading feathers (Ichida et al 2001;Okoroma et al 2012;Langeveld et al 2003;Fakhfakh et al 2009), wool and animal hide (Tiwary and Gupta 2010;Desai et al 2010) and PrP SC prion (Langeveld et al 2003;Yoshioka et al 2007;Okoroma et al 2013). From the Gram-negative category, keratinases produced by members of the Chryseobacterium or Stenotrophomonas genera have been widely studied and shown to degrade feather (Chaudhari et al 2013;Gurav amd Jadhav 2013;Cao et al 2009;Jeong et al 2010), animal hair (Gurav and Jadhav 2013;Cao et al 2009), wool (Fang et al 2013;Cao et al 2009), hoof andhorn (Cao et al 2009;Yamamura et al 2002).Some thermophilic anaerobic bacteria also demonstrated an ability to produce serine type keratinases. Fervidobacterium pennavorans (Friedrich and Antranikian 1996) and F. islandicum (Nam et al 2002;Kluskens et al 2002) were isolated from hot springs and produced keratinases that can degrade feathers efficiently.…”

Section: Sources Of Microbial Keratinasesmentioning

confidence: 99%

“…nigar that cause diseases in valuable plants and crops (Jeong et al 2010). Similarly, keratinase produced by Thermoactinomyces also showed antifungal properties against these plant pathogens (Gousterova et al 2012).…”

Section: Biological Controlmentioning

confidence: 99%

Microbial keratinases: characteristics, biotechnological applications and potential.

Purchase

2016

The Handbook of Microbial Bioresources

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Keratinases are a group of proteolytic enzymes that can catalyse the cleavage and hydrolysis of the highly stable and fibrous proteins: keratins. A diverse range of microorganisms, including fungi, actinomycetes and bacteria, have been reported to produce keratinases that have biotechnological applications and potential. These keratinases have been usefully applied in agricultural, pharmaceutical, leather and textile processes as well as within environmentally friendly waste management solutions. Potential uses of keratinases include the fields of biomedicine, cosmetics, biological control and the generation of green energy. Herein we aim to provide an overview of the properties of this group of versatile enzymes, including the mechanisms of keratin degradation. The diversity of microbial sources of keratinases is discussed and the optimisation of keratianse production examined.We conclude with an assessment of the established biotechnological applications of keratinases in different industries and current research that highlights other promising potential uses.

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Feather degrading, phytostimulating, and biocontrol potential of native actinobacteria from North Eastern Indian Himalayan Region

et al. 2018

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Twelve actinobacterial strains were isolated from tomato rhizospheric soil from Manipur, a state in North East Indian Himalayan Region and screened for keratinolytic and plant growth promoting traits. Nine promising isolates were identified as Streptomyces species using partial 16S rRNA gene sequencing. Among the seven isolates showing chicken feather degradation activity, three keratinolytic strains RCM-SSR-2, -6, and -12 were found to be the most efficient feather degrading strains achieving 90% feather weight loss within 48 h of incubation. They also showed maximum keratinase and soluble peptide production. Strain RCM-SSR-2, -5, -6, -8, and -11 showed positive results for all plant growth promoting traits tested. Maximum indole-3-acetic acid production was exhibited by RCM-SSR-6. Strain RCM-SSR-1, -2, -5, -6, -9, and -11 showed antagonistic activity against three important plant pathogens. Feather hydrolysate of RCM-SSR-6 was also evaluated for in vitro seed germination test using garden pea seeds. Higher concentration of feather protein hydrolysate (3 mg ml ) inhibited shoot and root length of the germinating embryo. However, lower concentration (0.01 mg ml ) of feather protein hydrolysate promoted seed germination. Among the 12 strains, four isolates namely RCM-SSR-1, -2, -5, and -6 were found to be promising as multi-traits plant growth promoting rhizobacteria for development of organic fertilizer, phytostimulator, and biocontrol agents.

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Production of keratinolytic enzyme by a newly isolated feather-degrading Stenotrophomonas maltophilia that produces plant growth-promoting activity

Cited by 95 publications

References 35 publications

Ameliorating effects of chicken feathers in plant growth promotion activity by a keratinolytic strain of Bacillus subtilis PF1

Ameliorating effects of chicken feathers in plant growth promotion activity by a keratinolytic strain of Bacillus subtilis PF1

Microbial keratinases: characteristics, biotechnological applications and potential.

Feather degrading, phytostimulating, and biocontrol potential of native actinobacteria from North Eastern Indian Himalayan Region

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