Production of intra‐ocular hemorrhage by mouse trophoblast

Grobstein, Clifford

doi:10.1002/jez.1401140208

Cited by 27 publications

(5 citation statements)

References 8 publications

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“…Studies on the differentiation of cells of the ectoplacental cone and extraembryonic ectoderm of rodents have shown that when ectoplacental cones or extraembryonic ectoderm are isolated and cultured in vitro (Ansell and Snow, 1975;Rossant and Ofer, 19771, they originate only trophoblastic giant cells similar to giant cells of the rodent placenta. When the same tissues are implanted in ectopic sites, they give origin to giant cells associated with an extensive hemorrhagic area of the host tissue (Grobstein, 1950;Billington, 1965;Rossant and Ofer, 1977). No other cell types could be detected in both kinds of experiments.…”

Section: Discussionmentioning

confidence: 99%

Growth of mouse ectoplacental cone cells in subcutaneous tissues. Development of placental‐like cells

1991

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Ectoplacental cones of mouse embryos collected on day 8 of pregnancy were grafted into the dorsal subcutaneous tissue of host mice. The grafts were collected between days 3 and 8 after transfer and processed for light and electron microscope morphological analysis as well as for cytochemistry of nonspecific alkaline phosphatase. Fragments of normal mouse placentas collected between days 12 and 18 of pregnancy were processed similarly. About 37% of the grafts were nonhemorrhagic nodules formed by different kinds of trophoblastic cells. These cells had many morphological and cytochemical features of cells present in normal mouse placentas. Nonphagocytic giant cells, glycogen cells, as well as cells with a well-developed granular endoplasmic reticulum were similar to cells found in the placenta and were always present in the grafts. Cells showing features intermediate between the above-mentioned cells and those whose cytoplasm was poor in organelles also were found in the grafts. The latter resembled cells of layer 1 of the labyrinth of the placenta. These results suggest that trophoblastic cells of the ectoplacental cones had differentiated into placental cells following their transfer to the subcutaneous tissue.

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Section: Discussionmentioning

confidence: 99%

Growth of mouse ectoplacental cone cells in subcutaneous tissues. Development of placental‐like cells

1991

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“…Approximately 3 x 106 cells were inoculated into each mouse. Two of the tumours were implanted intraperitoneally and two intraocularly, using the method of Grobstein (1950). In all 17 primary tumours were established from different transfer generations of the three embryo lines, 24 from different transfer generations of the six young cell lines and 27 from different transfer generations of the ten old cell lines.…”

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

The Structure of Tumours Derived from Mouse Cells after “Spontaneous” Transformation in vitro

Franks¹,

Chesterman²,

Rowlatt³

1970

Br J Cancer

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SUMMARY.-The histological structure of 58 primary tumours derived from spontaneously transformed tissue culture cell lines from embryonic (14-16 days), young (3-20 days) and old (28-34 months) C3H and C57 mice is described. Although the cell lines were derived from a number of different organs the tumours were similar in morphology. The pattern was mixed, with " fibrosarcomatous ", " myxoid ", " epithelioid " and giant cell areas. The tumours resemble some types of haemangiopericytoma.ALTHOUGH spontaneous neoplastic transformation in vitro has been studied by many workers there have been relatively few detailed studies on the nature of the tumours developing after implantation of transformed cells into syngeneic mice. In most cases these have been classified as " fibrosarcomas " (see e.g. Nettleship et al., 1943;Evans et al., 1964;Cornell, 1969). The purpose of this paper is to describe the morphology of such tumours which arose from cell lines established from a number of different organs from young and old C57 and C3H mice. The establishment of the cell lines and the ultrastructural morphology of the tissue culture cells have been described in earlier papers (Franks and Henzell, 1970;Franks and Wilson, 1970). The histochemistry, enzyme biochemistry and ultrastructure of the tumours will be described in a later paper. MATERIAL AND METHODSNineteen tumour-producing cell lines were established from embryo (13-18 days), young (3-20 days) and old (28-34 months) C3H and C57BL at Icrf mice. Details of the tissue culture methods used for the young and old mice are described in an earlier paper (Franks and Henzell, 1970). The embryo lines were established by Dr. S. Lan from whole embryos, using methods described by Todaro and Green (1963). The other lines were derived from the following organs-kidney, bladder, lung, tongue, heart, prostate, brain, spinal cord and nerve. The tissue culture cells were removed from their containers by the method by which they were usually transferred, i.e. trypsinisation or scraping, and centrifuged. The pellet was resuspended in about 0-6 ml. of tissue culture medium. Routinely 0-2 ml. of the suspension was injected subcutaneously into syngeneic hosts 3-6 months old. Approximately 3 x 106 cells were inoculated into each mouse. Two of the tumours were implanted intraperitoneally and two intraocularly, using the method of Grobstein (1950). In all 17 primary tumours were established from different

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“…The development of the specific chemically defined medium and the method of initiation of tissue culture line NCTC 4705 from pooled minced cells of 22 embryos removed from three C3H/ HeN females after 13 days in utero have been described in detail elsewhere (2,18). Intraocular implants from cell cultures 910 days in vitro were made into five irradiated (425 r, whole-body exposure) weanling C3H/HeN mice by the technique of Grobstein (22). From one of these, a l-mm3 portion of a MURINE C-TYPE VIRUS resulting tumor was then implanted into the left thigh muscles of each of three mice of the same age and strain.…”

Section: Methodsmentioning

confidence: 99%

In Vitro and In Vivo Observations on a Murine C-Type Virus

Hall

Andresen

Evans

1967

J Virol

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From 40 discrete mouse tissue culture cell lines examined by electron microscopy or complement fixation, or both, for the presence of detectable virus, one (NCTC 4705), initiated and maintained on chemically defined medium, was chosen for a more extensive study. Virus-like particles (100 to 110 m,u), morphologically similar to previously reported immature and mature C-type leukemia virus particles, were

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Production of intra‐ocular hemorrhage by mouse trophoblast

Cited by 27 publications

References 8 publications

Growth of mouse ectoplacental cone cells in subcutaneous tissues. Development of placental‐like cells

Growth of mouse ectoplacental cone cells in subcutaneous tissues. Development of placental‐like cells

The Structure of Tumours Derived from Mouse Cells after “Spontaneous” Transformation in vitro

In Vitro and In Vivo Observations on a Murine C-Type Virus

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