In Vitro and In Vivo Observations on a Murine C-Type Virus

Hall, William S.; Andresen, William F.; Evans, Virginia J.

doi:10.1128/jvi.1.6.1238-1254.1967

Cited by 8 publications

(3 citation statements)

References 40 publications

(18 reference statements)

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“…Groups of particles could be seen external to the cells, but because some of the cells appeared to have degenerated, it is possible that many of the particles were released by a process (cell lysis, for example) other than budding. The majority of the particles appeared to have C-type particle morphology (10,21), measuring 100 to 135 nm in diameter, being enveloped, and having an electron-dense endocentric nucleoid (Fig. 1C).…”

Section: Resultsmentioning

confidence: 99%

Characterization of C-Type Particles Produced by a Tissue Culture-Adapted Murine Myeloma

Volkman

Krueger²

1973

J Virol

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C-type particles produced by a tissue culture-adapted BALB/c myeloma were characterized. It was determined that although the particles were morphologically and antigenically similar to murine leukemia and sarcoma virus, the size of their RNA was different, they lacked RNA-dependent DNA polymerase, they were unstable in NET buffer, sucrose and citrate but were stable in glycerol and Earle balanced salt solution, and they behaved differently from oncornaviruses when treated with ether and detergent. Murine myelomas in culture have been reported to produce an RNA-containing virus with antigenicity, morphology, nucleic acid type (RNA), size (74S), and base composition similar to murine leukemia virus (MuLV) (43). The myeloma viruses Watson et al. (43) studied were all from oil-induced tumors except for those from the C-1 cell line, a spontaneous myeloma of a C3H mouse. Aoki et al. (3) showed that the C-type particle produced by MOPC-70A possessed the G (Gross) viral envelope antigen (GVEA) and XVEA, a viral enve

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Section: Resultsmentioning

confidence: 99%

Characterization of C-Type Particles Produced by a Tissue Culture-Adapted Murine Myeloma

Volkman

Krueger²

1973

J Virol

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“…Table 2 shows results identical with those of Swiss mouse embryo tissue culture passages: no A particles visible in any of the cultures, and C particles present in NCTC 3069 and 2472. A previous study (22,23) had shown that a similar C-type particle isolated from NCTC 4705, also derived from C3H mouse cells, actually grew better in NIH-METC than in the C3H cells of origin.…”

Section: Cell Strain Mediummentioning

confidence: 91%

Characteristics of and Relationship Between C Particles and Intracisternal A Particles in Cloned Cell Strains

Hall

Hartley

Sanford

1968

J Virol

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Four murine tissue culture cell strains, which originated by cloning from one common cell of subcutaneous connective tissue origin, were examined for the presence of virus by electron microscopy and complement fixation techniques. The relative distribution of C particles and intracisternal A particles was determined. Thereafter, the characteristics of and relationship between A- and C-type particles were investigated. Cell extracts were passaged onto virus-free Swiss and C3Hf mouse embryo tissue cultures; CF and EM tests were again made to investigate the infective capacities of the various particle types. Although C particles were found in passage cultures exposed to extracts from the C particle-bearing strains, no A particles were found in any passage culture. These results indicate that the intracisternal A particle was neither infective nor developmentally associated with the C particle. A positive CF test was correlated with the presence of morphologically detectable C particles and was independent of the concentration of A particles.

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“…The origin and nature of these viruses are not known. However, it was demonstrated that they are infectious, can be transmitted in vifro and induce in the infected cells the Gross leukemia type antigens (Hall et a/., 1967;Hall et a/., 1968;Barski and Youn, 1971). On the other hand, it was shown that the action of chemical carcinogens can, at least in vivo, apparently bring about an activation of latent C-type virus infection (Igel et al, 1969).…”

Section: Discussionmentioning

confidence: 99%

Decrease in tumor‐producing capacity of mouse cell lines following infection with mouse leukemia viruses

Barbieri

Belehradek

Barski

1971

Intl Journal of Cancer

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Two neoplastic cell strains derived from the CS7BL/6 mice, P4bis originating from normal adult lung tissue '' spontaneously " transformed in vitro, and TBLC2 developed from a methylcholanthrene-induced sarcoma, were both infected in vitro with Rauscher leukemia virus. Consecutive to this infection, the tumor-producing capacity of these cells, which continued to multiply normally in vitro, dropped considerably when checked in syngeneic mice. This was evidenced by a dramatic decrease of takes and a significant prolongation of latency period. The high leukemogenic R R L f and the low leukemogenic RCL-virus variants were equally ejicient in infecting cells in vitro andproducing a decrease of their tumorigenic potential in vivo. This decrease coincided with the massive appearance in the cells and culture supernatant of C-type particles and of a new surface antigen reacting specifically in the immunofluorescence test with anti-Rauscher virus serum. The specific immunological nature of decreased tuniorigenicity was confirmed by the fact that the rejection of the virus-infected cells could be prevented by total irradiation of intact recipient animals, whereas animals preimmunized and laterirradiated continued to reject the infected cells. On the other hand, the rare tumors which appeared in non-irradiated adults after a long latency period were composed of virus-fuee cells whereas cells fvom tumors obtained by inoculation of new-born animals regularly contained the virus and were rejected when grafted on normal adults.

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In Vitro and In Vivo Observations on a Murine C-Type Virus

Cited by 8 publications

References 40 publications

Characterization of C-Type Particles Produced by a Tissue Culture-Adapted Murine Myeloma

Characterization of C-Type Particles Produced by a Tissue Culture-Adapted Murine Myeloma

Characteristics of and Relationship Between C Particles and Intracisternal A Particles in Cloned Cell Strains

Decrease in tumor‐producing capacity of mouse cell lines following infection with mouse leukemia viruses

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