Production of inflammatory mediators by human macrophages obtained from ascites

Pruimboom, W. M.; Dijk, A. P. M. van; Tak, C. J. A. M.; Bonta, I. L.; Wilson, J. H. P.; Zijlstra, F. J.

doi:10.1016/0952-3278(94)90143-0

Cited by 12 publications

(9 citation statements)

References 46 publications

(19 reference statements)

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“…Köhler found that U937 sec re te d PGE 2 afte r inc ubation w ith TPA and arachidonic acid for 1-24 h. 26 It is know n that human pe ritone al mac rophage s do gene rate lipox ygenase produc ts afte r inc ubation w ith Ca-ionophore A23187. 13 How ever, in this study w e found no effect of A23187 (1 and 5 m M for 15 min) on U937 (data not show n). Pre vious studie s have reporte d the pre senc e of gluc ocortic oid rece ptors in U937 ce lls.…”

Section: Discussioncontrasting

confidence: 60%

“…Some studie s have inve stigate d the re gulation of cytokine s and eic osanoids in macrophage c ell line s or macrophages stimulate d w ith various inflammatory agents. [9][10][11][12][13] The human monoblastoid U937 tumour ce ll line is w idely use d as a model for the maturation of monocytic c ells. 14 U937 is an e stablishe d human monoblast c ell line de rive d from the ple ural ex udate of a patie nt w ith diffuse histioc ytic lymphoma.…”

Section: Introductionmentioning

confidence: 99%

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Time Dependent Production of Cytokines and Eicosanoids by Human Monocytic Leukaemia U937 Cells; Effects of Glucocorticosteroids

Garrelds

Hal

Haakmat

et al. 1999

Mediators of Inflammation

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In the present study the human monoblast cell line U937 has been used as a model to study the function of human mononuclear phagocytes in asthma. The kinetics of the production of eicosanoids and cytokines, which are thought to play a role in the pathogenesis of asthma, were studied. In addition, the effects of glucocorticosteroids were investigated, as these drugs are of great importance for the treatment of asthmatic patients. After stimulation with phorbol-12 myristate acetate (PMA) for 24 h, U937 cells were cultured in the absence or presence of lipopolysaccharide (LPS: 1 and 5 microg ml(-1)) and glucocorticosteroids (budesonide, fluticasone propionate and prednisolone: 10(-11), 10(-9) and 10(-7) M) for 96 h. The production of interleukin-1beta (IL-1beta), interleukin-6 (IL-6), prostaglandin E2 (PGE2) and thromboxane B2 (TxB2) gradually increased in time after stimulation with LPS, whereas the transient production of tumor necrosis factor alpha (TNF-alpha) reached its maximum between 6 and 12 h. Interferon-gamma (IFN-gamma), interleukin-10 (IL-10) and leukotriene B4 (LTB4) were not detectable. All three glucocorticosteroids (budesonide, fluticasone propionate and prednisolone) completely inhibited the production of both eicosanoids and cytokines. The production of eicosanoids was more sensitive to these glucocorticoids than the production of cytokines. The observed differences in the kinetics of the production of eicosanoids and cytokines stress the importance of time course experiments in studies on the effect of drugs on mononuclear cells.

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Section: Discussioncontrasting

confidence: 60%

Section: Introductionmentioning

confidence: 99%

Time Dependent Production of Cytokines and Eicosanoids by Human Monocytic Leukaemia U937 Cells; Effects of Glucocorticosteroids

Garrelds

Hal

Haakmat

et al. 1999

Mediators of Inflammation

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“…We also measured the pro-inflammatory cytokines interleukin-1 p (IL-1 p), IL-6 and tumour necrosis factor-CL (TNF-a, Arai et al, 1990;Oppenheim et al, 1991) which are also produced by hp-MQ, (Pruimboom et al, 1994).…”

mentioning

confidence: 99%

Levels of soluble intercellular adhesion molecule 1, eicosanoids and cytokines in ascites of patients with liver cirrhosis, peritoneal cancer and spontaneous bacterial peritonitis

Pruimboom

Bac

Dijk

et al. 1995

International Journal of Immunopharmacology

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The levels of the eicosanoids leukotriene B4, prostaglandin E2, prostacycline and thromboxane B2, the cytokines interleukin-1 beta, interleukin-6 and tumour necrosis factor-alpha and soluble intercellular adhesion molecule 1 were measured in ascites and plasma samples of patients with liver cirrhosis (53), peritoneal cancer (26) and spontaneous bacterial peritonitis (10) to assess their value as a possible diagnostic and prognostic parameter in the course of the disease. Soluble intercellular adhesion molecule 1, of the eicosanoids prostaglandin E2 and leukotriene B4, and the protein concentration in ascites were all significantly elevated in ascites of patients with peritoneal cancer in comparison to ascites of patients with liver cirrhosis. In ascites of patients with spontaneous bacterial infection interleukin-6 concentration was significantly elevated and the protein concentration was significantly lower in comparison to the other two groups. None of these parameters, however, seems to be of practical use as a diagnostic parameter, as there is an overlap between all the levels of these mediators in ascites of liver cirrhosis, peritoneal cancer and spontaneous bacterial peritonitis group. Soluble intercellular adhesion molecule 1 levels were much higher in plasma than in ascites, in contrast to interleukin-6 levels which were much higher in ascites than in plasma. Soluble intercellular adhesion molecule 1 in ascites correlated with soluble intercellular adhesion molecule 1 in plasma (r = 0.6926, P = 0.0001). Soluble intercellular adhesion molecule 1, interleukin-6 and the number of polymorphonuclear cells in peritoneal fluid correlated during episodes of infection in patients with a peritonitis. For this reason soluble intercellular adhesion molecule 1 and interleukin-6 could be of prognostic value for patients with peritonitis.

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“…After the discovery of leukotrienes, their biological activity [9] and role in inflammatory cells were investigated [10–12]. As a next step, the involvement of stimulated macrophages and their substances released were investigated as tools against tumour growth [13–17]. However, not only animal and human cells were subjected to immunopharmacological research activities.…”

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confidence: 99%

In Memoriam of Iván Lásló Bonta (1922–2007)

Zijlstra¹

2007

Mediators of Inflammation

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Production of inflammatory mediators by human macrophages obtained from ascites

Cited by 12 publications

References 46 publications

Time Dependent Production of Cytokines and Eicosanoids by Human Monocytic Leukaemia U937 Cells; Effects of Glucocorticosteroids

Time Dependent Production of Cytokines and Eicosanoids by Human Monocytic Leukaemia U937 Cells; Effects of Glucocorticosteroids

Levels of soluble intercellular adhesion molecule 1, eicosanoids and cytokines in ascites of patients with liver cirrhosis, peritoneal cancer and spontaneous bacterial peritonitis

In Memoriam of Iván Lásló Bonta (1922–2007)

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