Production of hydrocarbon-degrading microorganisms using agricultural residues of Mangifera indica L. and Carica papaya as carbon source

Abstract

The aim of the present study was to evaluate the potential of oils from agricultural residues, such as Mangifera indica L. (mango) and Carica papaya (papaya) from the Papaloapan region, Mexico, as a carbon source for the production of hydrocarbon-degrading (hydrocarbonoclastic) microorganisms in an airlift bioreactor via a common metabolic pathway for hydrocarbons and fatty acids. Biomass growth and carbon source uptake were measured using optical density and gas chromatography, respectively. Gompertz, logistic, and Von Bertalanffy mathematical models were used to obtain kinetic parameters such as the lag phase, maximum specific growth, and consumption rate. The hydrocarbonoclastic consortium was able to grow using papaya (6.09 ± 0.23 g L −1) and mango (2.59 ± 0.30 g L −1) oils, which contain certain antibacterial fatty acids. Differences observed in maximum specific growth and consumption rates indicate that, although mango oil was consumed faster (0.33 day −1 for mango and 0.25 day −1 for papaya), papaya oil provided a higher rate of biomass production per microorganism (0.24 day −1 for mango and 0.44 day −1 for papaya). Additionally, the consortium was able to consume 13 g L −1 diesel as a sole carbon source and improve its maximum specific consumption rate following growth using the oils. Furthermore, the maximum specific growth rate was decreased, indicating a change in the consortium capabilities. Nevertheless, agricultural waste oils from the Papaloapan region can be used to cultivate hydrocarbonoclastic microorganisms. The present study creates the possibility of investigating carbon sources other than hydrocarbons for the production of hydrocarbonoclastic microorganisms.

Comparison of the Antibacterial Activity of the Ethanol Extract vs Hydroalcoholic Extract of the Leaves of Mangifera indica L. (Mango) in Different Concentrations: An In Vitro Study

“…Since it is not possible to use first-line antibiotics, it is necessary to use more expensive medicines, which increases the costs for the family and society. [5][6][7][8] In health sciences, for example, the use of antibiotics is justified in the presence of odontogenic infections and as prophylaxis in at-risk patients. Among stomatological infections, bacteremia and osteomyelitis are common as a result of invasive dental procedures whose multifactorial origin includes a very varied microflora, finding opportunistic microorganisms such as S. aureus, which is related to the onset of these diseases.…”